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The map label for this gene is metG

Identifier: 15921721

GI number: 15921721

Start: 1435295

End: 1437010

Strand: Direct

Name: metG

Synonym: ST1432

Alternate gene names: 15921721

Gene position: 1435295-1437010 (Clockwise)

Preceding gene: 15921719

Following gene: 15921723

Centisome position: 53.26

GC content: 32.17

Gene sequence:

>1716_bases
ATGAAGATATTTGTAGCCTCTGCATGGCCTTATGTAAATGCAGTACCACATCTAGGTAATTTGATTGGATCAGTATTATC
TGCTGATGTTTTTGCTAGATATGCAAGGTTAAAATATGGCCAAGAGAATGTGGTATTTGTAAGTGGAAGTGACGAGCACG
GAACACCAATTGAGGTAGAAGCTAAGAAGAGAAATGTGAATCCTAAAGAGCTAACCGATCAAGCACATGAATACGATAAA
AAGTTATTTTTAGATGTGTGGGAGATTAGTTATAATAATTACACTAGAACAGAATCAGAAATTCATAAAACTTTTGTCAG
AGATTTTATGTTAAAACTAGAAAAATATATAAAGATTGAAGAAGATGAAATTCCATATTGTGAGTATGATAAAATTTACT
TACCAGATAGATTTGTGAAAGGTACTTGTCCTTATTGCGGATTTGAAGATGCAAGAGGAGATCAATGCGATAATTGTGGT
AGACTATTAACGCCAAGACTTCTAGTTAATCCTAAGTGCGTATTATGTGGAAGAACGCCAGTATTTAAGAAAACTAAACA
CTGGTTTTTTGATCTTTCAGCATTTAATGATAAAATTGAGGAATGGATTAAGAATTCTCAGACACTACCTGAAAATGTTA
AGTCAGTAGCCTTAAGTTGGGTTAAAGAAGGGCTAAAGCCAAGAAGCATTACGAGAGATAATGCTTGGGGTATTCCAGCA
CCTTTCGAAGGTGCTGAAGGTAAAACTATCTATGTATGGTTTGAGGCATTATTAGGTTATATCTCTGCTACGATAGAATA
TTTTAAGAAGATAGGAAAAGAAGAAGAGTGGAAGAAATTCTGGTTTGGAAATGATGTTAAAAGTTATTACTTTATTGGAA
AGGATAACATTCCATTCCATGCTGTAATATTACCAGCAATGCTAATGGCTTCTGGAGAAAATTATGTTTTACCTACAGTT
ATAGCGGCAACAGAATATTTGCTTTATGAAGGGCAAAAATTCAGTAAAAGTAGGAAAATTGGGGTATGGATTGATGAAGC
TCCTCAATTATTAGATATAGAATACTGGAGATTTATATTGATTAGACTTAGACCAGAAGAGAGAGACACTAATTTTACAT
GGAGAGAAGCACTAAGGATAGTTAATACAGAATTAAATGATGATATAGGTAACTATGCAAATAGAGTATTGAGTATGGTA
AGAAGATACTTTAATGGAGAGGTTCCTCAAATAAAATATGAAAAACTAAAAGATGAGGATACTAAATTTATTAGTGAAAT
AAAAGAAGCTCCTAAAAAGATGAGTGAATTATTTGAGCTAGGAAAATTAAAGGCCGGAAGTGAAGAAATATTAAAGTTAG
CTAGAAATGGTAACTCATATTTAAACATAAGAGCACCTTGGAATTTGATAAAGAACGATAAAGAAGAAGCTGGGAATGTC
TTAAATATTGCTGTTAATTCTCTTAGGACATTATCTATCATGCTATATCCATTAATGCCAAAGAGTGCCGAAAAACTATA
TAACATGTTAGGGTTTAAAGATATAGAGAGAGAAAAATGGGATCTCGCTGGAGAACTTGTTATTAAGTCTAATCATAAAA
TTAATGAGGTAAGCGTTCTGTTTAAAAAAGTCGAACTAAATGAGAATGATATAAATAAAAAATTAGATGAAATAAGAAAG
AACTTAGAAAAAATAAGACCTACCTTATTGCGTTAA

Upstream 100 bases:

>100_bases
AAACGGATATTTTCTAAAGTCTAGTATTAGCACTTCCTTTAATAATTTGGTGGCAAAGTTTTTATAAGCTTATTACCTTT
ACGAAGGAATTAAGAGCAAT

Downstream 100 bases:

>100_bases
CTCAACATAAGTCATTACTGGTTCGAATTTTCTTCCTCTTCCCTCATAGAATTTACTAAACATTTCGTAGAAGTGAAGTC
TCATATCTTGGATAAATACT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKIFVASAWPYVNAVPHLGNLIGSVLSADVFARYARLKYGQENVVFVSGSDEHGTPIEVEAKKRNVNPKELTDQAHEYDK
KLFLDVWEISYNNYTRTESEIHKTFVRDFMLKLEKYIKIEEDEIPYCEYDKIYLPDRFVKGTCPYCGFEDARGDQCDNCG
RLLTPRLLVNPKCVLCGRTPVFKKTKHWFFDLSAFNDKIEEWIKNSQTLPENVKSVALSWVKEGLKPRSITRDNAWGIPA
PFEGAEGKTIYVWFEALLGYISATIEYFKKIGKEEEWKKFWFGNDVKSYYFIGKDNIPFHAVILPAMLMASGENYVLPTV
IAATEYLLYEGQKFSKSRKIGVWIDEAPQLLDIEYWRFILIRLRPEERDTNFTWREALRIVNTELNDDIGNYANRVLSMV
RRYFNGEVPQIKYEKLKDEDTKFISEIKEAPKKMSELFELGKLKAGSEEILKLARNGNSYLNIRAPWNLIKNDKEEAGNV
LNIAVNSLRTLSIMLYPLMPKSAEKLYNMLGFKDIEREKWDLAGELVIKSNHKINEVSVLFKKVELNENDINKKLDEIRK
NLEKIRPTLLR

Sequences:

>Translated_571_residues
MKIFVASAWPYVNAVPHLGNLIGSVLSADVFARYARLKYGQENVVFVSGSDEHGTPIEVEAKKRNVNPKELTDQAHEYDK
KLFLDVWEISYNNYTRTESEIHKTFVRDFMLKLEKYIKIEEDEIPYCEYDKIYLPDRFVKGTCPYCGFEDARGDQCDNCG
RLLTPRLLVNPKCVLCGRTPVFKKTKHWFFDLSAFNDKIEEWIKNSQTLPENVKSVALSWVKEGLKPRSITRDNAWGIPA
PFEGAEGKTIYVWFEALLGYISATIEYFKKIGKEEEWKKFWFGNDVKSYYFIGKDNIPFHAVILPAMLMASGENYVLPTV
IAATEYLLYEGQKFSKSRKIGVWIDEAPQLLDIEYWRFILIRLRPEERDTNFTWREALRIVNTELNDDIGNYANRVLSMV
RRYFNGEVPQIKYEKLKDEDTKFISEIKEAPKKMSELFELGKLKAGSEEILKLARNGNSYLNIRAPWNLIKNDKEEAGNV
LNIAVNSLRTLSIMLYPLMPKSAEKLYNMLGFKDIEREKWDLAGELVIKSNHKINEVSVLFKKVELNENDINKKLDEIRK
NLEKIRPTLLR
>Mature_571_residues
MKIFVASAWPYVNAVPHLGNLIGSVLSADVFARYARLKYGQENVVFVSGSDEHGTPIEVEAKKRNVNPKELTDQAHEYDK
KLFLDVWEISYNNYTRTESEIHKTFVRDFMLKLEKYIKIEEDEIPYCEYDKIYLPDRFVKGTCPYCGFEDARGDQCDNCG
RLLTPRLLVNPKCVLCGRTPVFKKTKHWFFDLSAFNDKIEEWIKNSQTLPENVKSVALSWVKEGLKPRSITRDNAWGIPA
PFEGAEGKTIYVWFEALLGYISATIEYFKKIGKEEEWKKFWFGNDVKSYYFIGKDNIPFHAVILPAMLMASGENYVLPTV
IAATEYLLYEGQKFSKSRKIGVWIDEAPQLLDIEYWRFILIRLRPEERDTNFTWREALRIVNTELNDDIGNYANRVLSMV
RRYFNGEVPQIKYEKLKDEDTKFISEIKEAPKKMSELFELGKLKAGSEEILKLARNGNSYLNIRAPWNLIKNDKEEAGNV
LNIAVNSLRTLSIMLYPLMPKSAEKLYNMLGFKDIEREKWDLAGELVIKSNHKINEVSVLFKKVELNENDINKKLDEIRK
NLEKIRPTLLR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=570, Percent_Identity=36.8421052631579, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI24308436, Length=537, Percent_Identity=27.9329608938547, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1788432, Length=561, Percent_Identity=29.7682709447415, Blast_Score=248, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=37.6576576576577, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645096, Length=532, Percent_Identity=28.7593984962406, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=355, Percent_Identity=29.2957746478873, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=568, Percent_Identity=37.1478873239437, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=36.9565217391304, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21358483, Length=561, Percent_Identity=24.5989304812834, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_SULTO (Q971C1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377390.1
- ProteinModelPortal:   Q971C1
- GeneID:   1459463
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1432
- NMPDR:   fig|273063.1.peg.1558
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   STOK273063:ST1432-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 66488; Mature: 66488

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 336-336

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVASAWPYVNAVPHLGNLIGSVLSADVFARYARLKYGQENVVFVSGSDEHGTPIEVE
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEE
AKKRNVNPKELTDQAHEYDKKLFLDVWEISYNNYTRTESEIHKTFVRDFMLKLEKYIKIE
HHCCCCCHHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEC
EDEIPYCEYDKIYLPDRFVKGTCPYCGFEDARGDQCDNCGRLLTPRLLVNPKCVLCGRTP
CCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEECCCC
VFKKTKHWFFDLSAFNDKIEEWIKNSQTLPENVKSVALSWVKEGLKPRSITRDNAWGIPA
CCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PFEGAEGKTIYVWFEALLGYISATIEYFKKIGKEEEWKKFWFGNDVKSYYFIGKDNIPFH
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCHH
AVILPAMLMASGENYVLPTVIAATEYLLYEGQKFSKSRKIGVWIDEAPQLLDIEYWRFIL
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCCEEHHHEEEEE
IRLRPEERDTNFTWREALRIVNTELNDDIGNYANRVLSMVRRYFNGEVPQIKYEKLKDED
EEECCCCCCCCCHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
TKFISEIKEAPKKMSELFELGKLKAGSEEILKLARNGNSYLNIRAPWNLIKNDKEEAGNV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHCCE
LNIAVNSLRTLSIMLYPLMPKSAEKLYNMLGFKDIEREKWDLAGELVIKSNHKINEVSVL
EEEHHHHHHHHHHEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHH
FKKVELNENDINKKLDEIRKNLEKIRPTLLR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIFVASAWPYVNAVPHLGNLIGSVLSADVFARYARLKYGQENVVFVSGSDEHGTPIEVE
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEE
AKKRNVNPKELTDQAHEYDKKLFLDVWEISYNNYTRTESEIHKTFVRDFMLKLEKYIKIE
HHCCCCCHHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEC
EDEIPYCEYDKIYLPDRFVKGTCPYCGFEDARGDQCDNCGRLLTPRLLVNPKCVLCGRTP
CCCCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEECCCC
VFKKTKHWFFDLSAFNDKIEEWIKNSQTLPENVKSVALSWVKEGLKPRSITRDNAWGIPA
CCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PFEGAEGKTIYVWFEALLGYISATIEYFKKIGKEEEWKKFWFGNDVKSYYFIGKDNIPFH
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCHH
AVILPAMLMASGENYVLPTVIAATEYLLYEGQKFSKSRKIGVWIDEAPQLLDIEYWRFIL
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCCCCEEHHHEEEEE
IRLRPEERDTNFTWREALRIVNTELNDDIGNYANRVLSMVRRYFNGEVPQIKYEKLKDED
EEECCCCCCCCCHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHH
TKFISEIKEAPKKMSELFELGKLKAGSEEILKLARNGNSYLNIRAPWNLIKNDKEEAGNV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHCCE
LNIAVNSLRTLSIMLYPLMPKSAEKLYNMLGFKDIEREKWDLAGELVIKSNHKINEVSVL
EEEHHHHHHHHHHEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHH
FKKVELNENDINKKLDEIRKNLEKIRPTLLR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479