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The map label for this gene is gatD

Identifier: 15921527

GI number: 15921527

Start: 1263800

End: 1265146

Strand: Reverse

Name: gatD

Synonym: ST1264

Alternate gene names: 15921527

Gene position: 1265146-1263800 (Counterclockwise)

Preceding gene: 15921528

Following gene: 15921525

Centisome position: 46.95

GC content: 32.15

Gene sequence:

>1347_bases
ATGTTAGAAGGTTATAGAGGTAAGGCATTAGAATTTTTATCCTCTCATAATGTTGATGTAGGAGATCTAATAGAGCTACA
AAAAGATGGGTTATCGATAAAAGGGGTAGTAATGCCAAGCTATTCTAAGGAAGACGATATAATAGTTATAAAATTAGATA
ATGGATATAATATAGGAGTTTCGATAAGTGGAATTTCTAACTTTAAATTAGTTGAAAAAAAGAGACAGAATATCCAGGTT
AATAAAGGAGAGCAAAAACCCCTAAAGGAAAAGAGCGAAGTTAAAATAATTAGTACAGGTGGAACGATAGTAAGTAAAGT
AGAATATGAGACTGGTGCTGTAAGACCAGCACTAACAACTGAAGAGATTATAAATTTTATGCCCGAAATAAATGAGATTG
CAAAAATAGATGCAGAAGTATTATTTTCTATCCTAAGTGAAAATATGAAACCTGAATATTGGATTAAAATAGCAGAAAGT
GCAAAGAAAGCCTTAGATGAAGGAAATTTAGGCGTTGTAATTGCTCATGGCACAGACACTATGGCTTATACTGCTTCAGC
TTTAGCTTTTTCATTTAAATCTCTAACCGGACCTATAGTTCTTGTAGGATCTCAGAGAAGTAGTGATAGACCTAGCAGTG
ACTCTCCAATAAATCTCTATTCTGCTATACTAGTAGCTAAAAATTCTCCATTTGCAGAAGTGACTATCAATATGCATGGA
GAAAGCTCAGATACATATACTTTAGTACATAGAGGAGTAAAAGTAAGAAAAATGCATACTAGTAGAAGAGATGCTTTTCA
ATCTATAAATGATATACCCTTAGCGAAAGTTTTTTGGAAGGAAAAAGAAATTAAACTTCTGAGAAATGATTATATTAAAA
GAAAAGAAGAAAACGCTTTAGATGCAAAATTCGATACAAGAGTATTCCTATTAAAATACTATCCTGGTATTAATCCTGAA
ATAATAGAATATCTAATTTCTTCTGGAATTAGAGGAATAATAGTTGAGGGAACAGGATTAGGTCACACATCTACTGAATT
TGTAGACTATTTCAAAAAAGCAACTAAAGATGGAGTATTTATAGGAATGACGTCACAATGCTTATTTGGAAGAGTAAATA
TGAACGTTTATACTACGGGAAGATTATTGCAAGAATCTGGTGTTACTCCATTAGAAGATATGTTACCAGAAACAGCACTT
GTAAAACTTATGTGGGTTCTTGCACATGAATCTGATTTAGATAAAATAAGAAGTTTAATGCTTACTAATTTTGTAGGTGA
AATAAACTATAGGCACGTACCAGAATACTTTCCAAGGTGGTTCCATGACGGAATTAGATTACAGTAA

Upstream 100 bases:

>100_bases
TAGAATTGAATATGAGAAGAGAGTTGTAAAAACTAGACAGTCAAAACAAGCTGTAGCTGCTAGGTAAACGTTTTTATCTA
TTTTTATATCTTTTATTTTA

Downstream 100 bases:

>100_bases
AATAGGATTAAAAGTTGGTCTTGAAATTCACCAGCAATTAAATACAGCTCATAAACTATTTTGCGAATGTCCTACAACAC
TTCATGAAGAATATCACACA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MLEGYRGKALEFLSSHNVDVGDLIELQKDGLSIKGVVMPSYSKEDDIIVIKLDNGYNIGVSISGISNFKLVEKKRQNIQV
NKGEQKPLKEKSEVKIISTGGTIVSKVEYETGAVRPALTTEEIINFMPEINEIAKIDAEVLFSILSENMKPEYWIKIAES
AKKALDEGNLGVVIAHGTDTMAYTASALAFSFKSLTGPIVLVGSQRSSDRPSSDSPINLYSAILVAKNSPFAEVTINMHG
ESSDTYTLVHRGVKVRKMHTSRRDAFQSINDIPLAKVFWKEKEIKLLRNDYIKRKEENALDAKFDTRVFLLKYYPGINPE
IIEYLISSGIRGIIVEGTGLGHTSTEFVDYFKKATKDGVFIGMTSQCLFGRVNMNVYTTGRLLQESGVTPLEDMLPETAL
VKLMWVLAHESDLDKIRSLMLTNFVGEINYRHVPEYFPRWFHDGIRLQ

Sequences:

>Translated_448_residues
MLEGYRGKALEFLSSHNVDVGDLIELQKDGLSIKGVVMPSYSKEDDIIVIKLDNGYNIGVSISGISNFKLVEKKRQNIQV
NKGEQKPLKEKSEVKIISTGGTIVSKVEYETGAVRPALTTEEIINFMPEINEIAKIDAEVLFSILSENMKPEYWIKIAES
AKKALDEGNLGVVIAHGTDTMAYTASALAFSFKSLTGPIVLVGSQRSSDRPSSDSPINLYSAILVAKNSPFAEVTINMHG
ESSDTYTLVHRGVKVRKMHTSRRDAFQSINDIPLAKVFWKEKEIKLLRNDYIKRKEENALDAKFDTRVFLLKYYPGINPE
IIEYLISSGIRGIIVEGTGLGHTSTEFVDYFKKATKDGVFIGMTSQCLFGRVNMNVYTTGRLLQESGVTPLEDMLPETAL
VKLMWVLAHESDLDKIRSLMLTNFVGEINYRHVPEYFPRWFHDGIRLQ
>Mature_448_residues
MLEGYRGKALEFLSSHNVDVGDLIELQKDGLSIKGVVMPSYSKEDDIIVIKLDNGYNIGVSISGISNFKLVEKKRQNIQV
NKGEQKPLKEKSEVKIISTGGTIVSKVEYETGAVRPALTTEEIINFMPEINEIAKIDAEVLFSILSENMKPEYWIKIAES
AKKALDEGNLGVVIAHGTDTMAYTASALAFSFKSLTGPIVLVGSQRSSDRPSSDSPINLYSAILVAKNSPFAEVTINMHG
ESSDTYTLVHRGVKVRKMHTSRRDAFQSINDIPLAKVFWKEKEIKLLRNDYIKRKEENALDAKFDTRVFLLKYYPGINPE
IIEYLISSGIRGIIVEGTGLGHTSTEFVDYFKKATKDGVFIGMTSQCLFGRVNMNVYTTGRLLQESGVTPLEDMLPETAL
VKLMWVLAHESDLDKIRSLMLTNFVGEINYRHVPEYFPRWFHDGIRLQ

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=372, Percent_Identity=27.1505376344086, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1788065, Length=345, Percent_Identity=31.5942028985507, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789327, Length=321, Percent_Identity=26.791277258567, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI193207372, Length=290, Percent_Identity=34.1379310344828, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI193207374, Length=290, Percent_Identity=34.1379310344828, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558278, Length=290, Percent_Identity=34.1379310344828, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558280, Length=290, Percent_Identity=34.1379310344828, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320527, Length=159, Percent_Identity=35.2201257861635, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=261, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=261, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=261, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=261, Percent_Identity=26.4367816091954, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639609, Length=396, Percent_Identity=28.5353535353535, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645530, Length=377, Percent_Identity=28.6472148541114, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_SULTO (Q971W5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377196.1
- ProteinModelPortal:   Q971W5
- SMR:   Q971W5
- GeneID:   1459263
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1264
- NMPDR:   fig|273063.1.peg.1364
- HOGENOM:   HBG497495
- OMA:   PEFYHDE
- ProtClustDB:   PRK04183
- BioCyc:   STOK273063:ST1264-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 50231; Mature: 50231

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 102-102 ACT_SITE 178-178 ACT_SITE 179-179 ACT_SITE 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEGYRGKALEFLSSHNVDVGDLIELQKDGLSIKGVVMPSYSKEDDIIVIKLDNGYNIGV
CCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCEEEE
SISGISNFKLVEKKRQNIQVNKGEQKPLKEKSEVKIISTGGTIVSKVEYETGAVRPALTT
EECCCCCHHHHHHHHHCCEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCH
EEIINFMPEINEIAKIDAEVLFSILSENMKPEYWIKIAESAKKALDEGNLGVVIAHGTDT
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHCCCCEEEEEEECCCC
MAYTASALAFSFKSLTGPIVLVGSQRSSDRPSSDSPINLYSAILVAKNSPFAEVTINMHG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECC
ESSDTYTLVHRGVKVRKMHTSRRDAFQSINDIPLAKVFWKEKEIKLLRNDYIKRKEENAL
CCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCC
DAKFDTRVFLLKYYPGINPEIIEYLISSGIRGIIVEGTGLGHTSTEFVDYFKKATKDGVF
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEE
IGMTSQCLFGRVNMNVYTTGRLLQESGVTPLEDMLPETALVKLMWVLAHESDLDKIRSLM
EEECCHHEEEEECEEEEECCHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHH
LTNFVGEINYRHVPEYFPRWFHDGIRLQ
HHHHHHCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEGYRGKALEFLSSHNVDVGDLIELQKDGLSIKGVVMPSYSKEDDIIVIKLDNGYNIGV
CCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCEEEE
SISGISNFKLVEKKRQNIQVNKGEQKPLKEKSEVKIISTGGTIVSKVEYETGAVRPALTT
EECCCCCHHHHHHHHHCCEECCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCH
EEIINFMPEINEIAKIDAEVLFSILSENMKPEYWIKIAESAKKALDEGNLGVVIAHGTDT
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHCCCCEEEEEEECCCC
MAYTASALAFSFKSLTGPIVLVGSQRSSDRPSSDSPINLYSAILVAKNSPFAEVTINMHG
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECC
ESSDTYTLVHRGVKVRKMHTSRRDAFQSINDIPLAKVFWKEKEIKLLRNDYIKRKEENAL
CCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCC
DAKFDTRVFLLKYYPGINPEIIEYLISSGIRGIIVEGTGLGHTSTEFVDYFKKATKDGVF
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEE
IGMTSQCLFGRVNMNVYTTGRLLQESGVTPLEDMLPETALVKLMWVLAHESDLDKIRSLM
EEECCHHEEEEECEEEEECCHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHH
LTNFVGEINYRHVPEYFPRWFHDGIRLQ
HHHHHHCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479