Click here to switch to the map view.

The map label for this gene is nadA

Identifier: 15921460

GI number: 15921460

Start: 1199571

End: 1200536

Strand: Reverse

Name: nadA

Synonym: ST1197

Alternate gene names: 15921460

Gene position: 1200536-1199571 (Counterclockwise)

Preceding gene: 15921461

Following gene: 15921452

Centisome position: 44.55

GC content: 33.02

Gene sequence:

>966_bases
ATGCACAGAACACATGTTCGGGGATATATGCCAGACGCAGTGGAGTTAATAAAAGAAATAAAGAATTTAAAGAGAAATAA
AAACGCGATAATTTTAGGTCATAATTATATGGAGTATGGAGTACAATTAGTATCAGATTTCACTGGAGATTCTTATGATT
TAGCAGTAAAAGCAATGAAAACAAATGCAGACATTATAGTATTCGCTGGAGTATATTTTATGGCAGAACAAGCAGCAGCA
TTAAATCCAGATAAAAAAGTATTATCCCCAGATCCTAATGCCGGTTGCTCTTTATCGGATTCCTTAGATGTAGATACTTT
AAAAAAATATAAAGAAATGTATCCTAATGCTCCAGTAGTATTGTATATTAACACTAGTATTTACACGAAAGCATTAGCAG
ATTATATTGTAACTTCTTCAACTGCTATAAAAGTAGTGAAAAGTTTAGACGCAGACACAATAATATTCGGTCCAGACGCA
AATTTAGCAAATTACGTTGAGAGAAAAACTGGGAAGAGATTAGTAAAAGTGCCACCAAACGGAAGATGTATAGTCCATGC
CAATTACACAAGACAACTAGTTGAATTAGCAAGAAAGAAATATCCTAACGCAATACTCATGGCACATCCAGAAAGTCCAT
TAGAAATATTAGAAGCATCAGACTTTGTAGGCTCAACAAATCAAATGATAAAATTTGCTAAAGAAAGTCCCTATAAAGAA
TTCATAGTAGCTACAGAGTTAGGTATGATAAACGCATTAAAGTTGCAAGTACCAGAAAAAACATTCTACCCATTAGTTAC
TACTGAAGCTTATGCATGTGCAAGATGTCCTTATATGGCTATGATAACATTAGAAAAAATAAAAAGATCACTAGAAGAAG
AAATTTATGAAGTGAAAGTACCTAAGGATATAGCAGAAAGGGCAAAAGAAGCATTTGAAAGGACAATGAGACTACTAAAC
AACTAA

Upstream 100 bases:

>100_bases
AAGTGGTTATATAACCCATAGTTCAAGGTCATTAGACTTCTCTCTTGATGTATATAAATCATAAAAACAAATTTTCGATA
GTAATATTTAAAAACTTAAA

Downstream 100 bases:

>100_bases
CTTAAAATAAATCCTTTTTTCCCAATTAGGATCCTTATAAGGATAATCCTCCCTAAAATGATTACCCCTACTCTCCTTTC
TTATTTCAGCAGCTAATGCG

Product: quinolinate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MHRTHVRGYMPDAVELIKEIKNLKRNKNAIILGHNYMEYGVQLVSDFTGDSYDLAVKAMKTNADIIVFAGVYFMAEQAAA
LNPDKKVLSPDPNAGCSLSDSLDVDTLKKYKEMYPNAPVVLYINTSIYTKALADYIVTSSTAIKVVKSLDADTIIFGPDA
NLANYVERKTGKRLVKVPPNGRCIVHANYTRQLVELARKKYPNAILMAHPESPLEILEASDFVGSTNQMIKFAKESPYKE
FIVATELGMINALKLQVPEKTFYPLVTTEAYACARCPYMAMITLEKIKRSLEEEIYEVKVPKDIAERAKEAFERTMRLLN
N

Sequences:

>Translated_321_residues
MHRTHVRGYMPDAVELIKEIKNLKRNKNAIILGHNYMEYGVQLVSDFTGDSYDLAVKAMKTNADIIVFAGVYFMAEQAAA
LNPDKKVLSPDPNAGCSLSDSLDVDTLKKYKEMYPNAPVVLYINTSIYTKALADYIVTSSTAIKVVKSLDADTIIFGPDA
NLANYVERKTGKRLVKVPPNGRCIVHANYTRQLVELARKKYPNAILMAHPESPLEILEASDFVGSTNQMIKFAKESPYKE
FIVATELGMINALKLQVPEKTFYPLVTTEAYACARCPYMAMITLEKIKRSLEEEIYEVKVPKDIAERAKEAFERTMRLLN
N
>Mature_321_residues
MHRTHVRGYMPDAVELIKEIKNLKRNKNAIILGHNYMEYGVQLVSDFTGDSYDLAVKAMKTNADIIVFAGVYFMAEQAAA
LNPDKKVLSPDPNAGCSLSDSLDVDTLKKYKEMYPNAPVVLYINTSIYTKALADYIVTSSTAIKVVKSLDADTIIFGPDA
NLANYVERKTGKRLVKVPPNGRCIVHANYTRQLVELARKKYPNAILMAHPESPLEILEASDFVGSTNQMIKFAKESPYKE
FIVATELGMINALKLQVPEKTFYPLVTTEAYACARCPYMAMITLEKIKRSLEEEIYEVKVPKDIAERAKEAFERTMRLLN
N

Specific function: Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate

COG id: COG0379

COG function: function code H; Quinolinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the quinolinate synthase A family. Type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786964, Length=303, Percent_Identity=36.963696369637, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADA_SULTO (Q972D1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377129.1
- GeneID:   1459194
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1197
- NMPDR:   fig|273063.1.peg.1297
- HOGENOM:   HBG291157
- OMA:   IAHPECE
- ProtClustDB:   PRK09375
- BioCyc:   STOK273063:ST1197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00568
- InterPro:   IPR003473
- InterPro:   IPR023066
- TIGRFAMs:   TIGR00550

Pfam domain/function: PF02445 NadA

EC number: =2.5.1.72

Molecular weight: Translated: 36092; Mature: 36092

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: BINDING 34-34 BINDING 51-51 BINDING 122-122 BINDING 139-139 BINDING 209-209 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRTHVRGYMPDAVELIKEIKNLKRNKNAIILGHNYMEYGVQLVSDFTGDSYDLAVKAMK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEE
TNADIIVFAGVYFMAEQAAALNPDKKVLSPDPNAGCSLSDSLDVDTLKKYKEMYPNAPVV
CCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
LYINTSIYTKALADYIVTSSTAIKVVKSLDADTIIFGPDANLANYVERKTGKRLVKVPPN
EEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEECCC
GRCIVHANYTRQLVELARKKYPNAILMAHPESPLEILEASDFVGSTNQMIKFAKESPYKE
CEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHH
FIVATELGMINALKLQVPEKTFYPLVTTEAYACARCPYMAMITLEKIKRSLEEEIYEVKV
HHHHHHCCCEEEEEEECCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHEECC
PKDIAERAKEAFERTMRLLNN
CHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRTHVRGYMPDAVELIKEIKNLKRNKNAIILGHNYMEYGVQLVSDFTGDSYDLAVKAMK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEE
TNADIIVFAGVYFMAEQAAALNPDKKVLSPDPNAGCSLSDSLDVDTLKKYKEMYPNAPVV
CCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
LYINTSIYTKALADYIVTSSTAIKVVKSLDADTIIFGPDANLANYVERKTGKRLVKVPPN
EEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEECCC
GRCIVHANYTRQLVELARKKYPNAILMAHPESPLEILEASDFVGSTNQMIKFAKESPYKE
CEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHH
FIVATELGMINALKLQVPEKTFYPLVTTEAYACARCPYMAMITLEKIKRSLEEEIYEVKV
HHHHHHCCCEEEEEEECCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHEECC
PKDIAERAKEAFERTMRLLNN
CHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479