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The map label for this gene is yafV [C]

Identifier: 15921264

GI number: 15921264

Start: 1002684

End: 1003478

Strand: Direct

Name: yafV [C]

Synonym: ST1018

Alternate gene names: 15921264

Gene position: 1002684-1003478 (Clockwise)

Preceding gene: 15921263

Following gene: 15921269

Centisome position: 37.21

GC content: 32.45

Gene sequence:

>795_bases
ATGCGAATTGCTATTATTCAAACCTATATGACCTGGGATAAAAAGGATAATATAGAAAGACAAGTAGAATTAGTAAATAA
GGCAATAGATAATAAGGCAAAAATAATTGCATTAGATGAGTTAAGCAATACGATTTATTTTCCCTTTGAGCAAAATCCTA
AATATTTTAGCTGGGCAGAGACTGAAAGAGGAGAGACTCTACAGAGGTTTAAGGAAATAAGCAAAGAAAGAGAAGTTAGC
TTAATTGTACCAATTTTTGAGAGGGACAGTAATTTCTTTTACAATACTGCATTTATACTGGATAATGGGGAAATTATAGG
AAAATACAGAAAAACTCATTTGCCACAAGAGGAGTTTTTTAACGAATATTATTACTTTAAAGTTGGGGATTTAGGTTTTC
CAATATTTGATTTAAAAGGTGTAAAAACTGGAGTTGTTATTTGTCACGATAGGCATTTCCCAGAGCCTGTCAGAGTTGAA
GTAATTAAAGGAGCCTGGCTAATTTTCATACCTTCTGTTGCTGCATTTAAAGAGATTTGGGAATTGGAGCTTAAGGCTCA
TGCTGTTTTTAACACTGTATATATTGCTGGAATAAATAGGTTTGGTAAAGAATATCCTAATCAAAAAGAAGAGTATTTTG
GTGAAAGTATGATTATTTCGCCAATAGGCGAAATAGTTACTAAGGCTGAGAAAAATGAAGAGATATTATATGCTGATATT
TCTGAAGATGAAGTTATTAACGCGAGAATAAAAAGGCCTTTCCTAAAAAAGAGGCTATCAAGTTATGGGCTCTAA

Upstream 100 bases:

>100_bases
TATCAAAAGAATATAGACTTCGTCAAGGTATCGATATAAATTTAGCCTTTAAGTTAATACCACCAGAATAATTTATTTAC
TTTTTTTATGACTATATTTT

Downstream 100 bases:

>100_bases
AAATCTAACTTGCCTGAGTTAAATAGATCTTCTGGGTCAACTTTTCTCTTAAAGTTAATGATATATTTTAATCTTCTTTC
CTCAACTCTATCATTTATCT

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRIAIIQTYMTWDKKDNIERQVELVNKAIDNKAKIIALDELSNTIYFPFEQNPKYFSWAETERGETLQRFKEISKEREVS
LIVPIFERDSNFFYNTAFILDNGEIIGKYRKTHLPQEEFFNEYYYFKVGDLGFPIFDLKGVKTGVVICHDRHFPEPVRVE
VIKGAWLIFIPSVAAFKEIWELELKAHAVFNTVYIAGINRFGKEYPNQKEEYFGESMIISPIGEIVTKAEKNEEILYADI
SEDEVINARIKRPFLKKRLSSYGL

Sequences:

>Translated_264_residues
MRIAIIQTYMTWDKKDNIERQVELVNKAIDNKAKIIALDELSNTIYFPFEQNPKYFSWAETERGETLQRFKEISKEREVS
LIVPIFERDSNFFYNTAFILDNGEIIGKYRKTHLPQEEFFNEYYYFKVGDLGFPIFDLKGVKTGVVICHDRHFPEPVRVE
VIKGAWLIFIPSVAAFKEIWELELKAHAVFNTVYIAGINRFGKEYPNQKEEYFGESMIISPIGEIVTKAEKNEEILYADI
SEDEVINARIKRPFLKKRLSSYGL
>Mature_264_residues
MRIAIIQTYMTWDKKDNIERQVELVNKAIDNKAKIIALDELSNTIYFPFEQNPKYFSWAETERGETLQRFKEISKEREVS
LIVPIFERDSNFFYNTAFILDNGEIIGKYRKTHLPQEEFFNEYYYFKVGDLGFPIFDLKGVKTGVVICHDRHFPEPVRVE
VIKGAWLIFIPSVAAFKEIWELELKAHAVFNTVYIAGINRFGKEYPNQKEEYFGESMIISPIGEIVTKAEKNEEILYADI
SEDEVINARIKRPFLKKRLSSYGL

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=31.25, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI7706509, Length=227, Percent_Identity=29.5154185022026, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI5031947, Length=281, Percent_Identity=26.6903914590747, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI297632348, Length=281, Percent_Identity=26.6903914590747, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI297632350, Length=281, Percent_Identity=26.6903914590747, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=255, Percent_Identity=31.3725490196078, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17556280, Length=271, Percent_Identity=23.9852398523985, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=281, Percent_Identity=27.0462633451957, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358471, Length=268, Percent_Identity=28.3582089552239, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17933642, Length=253, Percent_Identity=28.4584980237154, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355835, Length=284, Percent_Identity=28.8732394366197, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 30994; Mature: 30994

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAIIQTYMTWDKKDNIERQVELVNKAIDNKAKIIALDELSNTIYFPFEQNPKYFSWAE
CEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECC
TERGETLQRFKEISKEREVSLIVPIFERDSNFFYNTAFILDNGEIIGKYRKTHLPQEEFF
CCCCHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEEECCCEEECHHHHCCCHHHHC
NEYYYFKVGDLGFPIFDLKGVKTGVVICHDRHFPEPVRVEVIKGAWLIFIPSVAAFKEIW
CCEEEEEECCCCCCEEECCCCCCCEEEEECCCCCCCEEEEEECCEEEEEECCHHHHHHHH
ELELKAHAVFNTVYIAGINRFGKEYPNQKEEYFGESMIISPIGEIVTKAEKNEEILYADI
HHHHHHHHHHHHHEEECHHHHHHHCCCCHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEC
SEDEVINARIKRPFLKKRLSSYGL
CCCHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRIAIIQTYMTWDKKDNIERQVELVNKAIDNKAKIIALDELSNTIYFPFEQNPKYFSWAE
CEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCEEEECC
TERGETLQRFKEISKEREVSLIVPIFERDSNFFYNTAFILDNGEIIGKYRKTHLPQEEFF
CCCCHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEEECCCEEECHHHHCCCHHHHC
NEYYYFKVGDLGFPIFDLKGVKTGVVICHDRHFPEPVRVEVIKGAWLIFIPSVAAFKEIW
CCEEEEEECCCCCCEEECCCCCCCEEEEECCCCCCCEEEEEECCEEEEEECCHHHHHHHH
ELELKAHAVFNTVYIAGINRFGKEYPNQKEEYFGESMIISPIGEIVTKAEKNEEILYADI
HHHHHHHHHHHHHEEECHHHHHHHCCCCHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEEC
SEDEVINARIKRPFLKKRLSSYGL
CCCHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]