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The map label for this gene is cca

Identifier: 15921188

GI number: 15921188

Start: 925180

End: 926421

Strand: Direct

Name: cca

Synonym: ST0952

Alternate gene names: 15921188

Gene position: 925180-926421 (Clockwise)

Preceding gene: 15921187

Following gene: 15921189

Centisome position: 34.33

GC content: 34.7

Gene sequence:

>1242_bases
ATGACAATAGAGGAGGAAGTTCTAAAGAGAATAAAACCTAGTAAAGAAGACGAGGAAAAGCTAAGGGAAAGAGCTCAGAT
TATATTAGATAGACTAAAGGGTTATAATGCAGAAATTGAGGGCTCATTCAGGAAAGGTACATGGCTAAAAGGAGATACTG
ATATAGATATTTTCGTATTTTTCCCTAAAAGTGTGGGTAAAGAATATCTTAGAGAGAAGGCTTTAAAGGAATTAATAGAA
AGATTCCGTGATTTGAACTATAAAATTGCCTATGCTGAACATCCTTATTTGATAGTGTACGTTGATGATGTTGAGATAGA
TGTGGTTCCGGCCTTAAGTATAGAGTCTGGAGAAGAGGCGATTACTGCAGCGGATAGAACCCCGTTTCACACTAAGTATG
TGATTTCTCATTTAGATGAAAAAGGGAGAGACGAAGTAAGACTATTAAAGAGATTTTTGAAAGGAATCGGAGTATATGGA
GCAGAGATTAAAGTTAAAGGATTTTCCGGTTATGTTACTGAGCTTTTAATAATCTATTATGGTTCATTCAAGGAAGTTCT
TAGAAATGCATCAAAGTTTCGTCCACCAGTTAGAATAGAATTAGTGAGACCAAAGAAAGAATTTGATTCCCCATTAATCG
TTCCCGATCCCGTAGATCCTAAAAGGAATGCATCATCAGCCGTATCATTAAAAAGCTTAGCAACTTTCGCTTTAGCTTCA
AAAATTTATCTTGAGAAACCTTCTATGGAATTCTTTTTTCCCTCTAAACCGGGTAGGCAAACAATAAAAGGTGATATTCT
TCTCGTGAAAGTGAAAATCGAGGAAAGTACTGTGGAAGATATAATATGGGGACAAGTATGGAGAAATGTGGAAAAGTTAA
AAACATTAATAAGTCATGAAGGCTATAGGGTTATTGATATTTCTGCTTGGGGGGATGCTGAGAATATAACTATTGGTATA
CAATTAGAAAGTAAGAGCATTGGTGAGTATTATCTAAATGTAGGTCCTTACTTTTATCTGCATAATGTAAAAAATTTCAT
AGAAGAAAATGAGAATGTATGGATTGGCGAAGACGGTAGGCTATACTCTATTAAGAGAAGAAGATATACAGTGGAAGAAA
TAATAAAAAGGAATTTATCCTTTAAGCAGAAGTTTACTTATGAATTACAGTGGCTTACTGAGGAAGTAGATGACCCTTGG
ATAAACTTATTCCTAAGAAAAACACCAAGTTGGTTAAAGTGA

Upstream 100 bases:

>100_bases
ATAGAGTTTGGAGAAATTATCGTTAATAAGATAATTTTATTTAAGAGTACCTTAACACCTAAAGGACCAATATATGACCC
ATTACTAGAAGTGACCTCTA

Downstream 100 bases:

>100_bases
GGGATTTCATTTATCCAAGATATGATGAAAATATTGAAAGAGAATTAATAGAACATGGTATAAAGGAACTTTACTCTTTT
GGTCCTATAAACTTAGGTAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTIEEEVLKRIKPSKEDEEKLRERAQIILDRLKGYNAEIEGSFRKGTWLKGDTDIDIFVFFPKSVGKEYLREKALKELIE
RFRDLNYKIAYAEHPYLIVYVDDVEIDVVPALSIESGEEAITAADRTPFHTKYVISHLDEKGRDEVRLLKRFLKGIGVYG
AEIKVKGFSGYVTELLIIYYGSFKEVLRNASKFRPPVRIELVRPKKEFDSPLIVPDPVDPKRNASSAVSLKSLATFALAS
KIYLEKPSMEFFFPSKPGRQTIKGDILLVKVKIEESTVEDIIWGQVWRNVEKLKTLISHEGYRVIDISAWGDAENITIGI
QLESKSIGEYYLNVGPYFYLHNVKNFIEENENVWIGEDGRLYSIKRRRYTVEEIIKRNLSFKQKFTYELQWLTEEVDDPW
INLFLRKTPSWLK

Sequences:

>Translated_413_residues
MTIEEEVLKRIKPSKEDEEKLRERAQIILDRLKGYNAEIEGSFRKGTWLKGDTDIDIFVFFPKSVGKEYLREKALKELIE
RFRDLNYKIAYAEHPYLIVYVDDVEIDVVPALSIESGEEAITAADRTPFHTKYVISHLDEKGRDEVRLLKRFLKGIGVYG
AEIKVKGFSGYVTELLIIYYGSFKEVLRNASKFRPPVRIELVRPKKEFDSPLIVPDPVDPKRNASSAVSLKSLATFALAS
KIYLEKPSMEFFFPSKPGRQTIKGDILLVKVKIEESTVEDIIWGQVWRNVEKLKTLISHEGYRVIDISAWGDAENITIGI
QLESKSIGEYYLNVGPYFYLHNVKNFIEENENVWIGEDGRLYSIKRRRYTVEEIIKRNLSFKQKFTYELQWLTEEVDDPW
INLFLRKTPSWLK
>Mature_412_residues
TIEEEVLKRIKPSKEDEEKLRERAQIILDRLKGYNAEIEGSFRKGTWLKGDTDIDIFVFFPKSVGKEYLREKALKELIER
FRDLNYKIAYAEHPYLIVYVDDVEIDVVPALSIESGEEAITAADRTPFHTKYVISHLDEKGRDEVRLLKRFLKGIGVYGA
EIKVKGFSGYVTELLIIYYGSFKEVLRNASKFRPPVRIELVRPKKEFDSPLIVPDPVDPKRNASSAVSLKSLATFALASK
IYLEKPSMEFFFPSKPGRQTIKGDILLVKVKIEESTVEDIIWGQVWRNVEKLKTLISHEGYRVIDISAWGDAENITIGIQ
LESKSIGEYYLNVGPYFYLHNVKNFIEENENVWIGEDGRLYSIKRRRYTVEEIIKRNLSFKQKFTYELQWLTEEVDDPWI
NLFLRKTPSWLK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_SULTO (Q973E7)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376857.1
- ProteinModelPortal:   Q973E7
- SMR:   Q973E7
- GeneID:   1458917
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0952
- NMPDR:   fig|273063.1.peg.1025
- HOGENOM:   HBG645243
- OMA:   DISHCIN
- ProtClustDB:   PRK13300
- BioCyc:   STOK273063:ST0952-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01264
- InterPro:   IPR006116
- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329
- PIRSF:   PIRSF005335
- TIGRFAMs:   TIGR03671

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2; SSF55003 PAP_C

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 48138; Mature: 48007

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50152 25A_SYNTH_3

Important sites: BINDING 42-42 BINDING 45-45 BINDING 130-130 BINDING 150-150 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEEEVLKRIKPSKEDEEKLRERAQIILDRLKGYNAEIEGSFRKGTWLKGDTDIDIFVF
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCCCEEEEEE
FPKSVGKEYLREKALKELIERFRDLNYKIAYAEHPYLIVYVDDVEIDVVPALSIESGEEA
ECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCEEEEEEEEECCCCCHH
ITAADRTPFHTKYVISHLDEKGRDEVRLLKRFLKGIGVYGAEIKVKGFSGYVTELLIIYY
EEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
GSFKEVLRNASKFRPPVRIELVRPKKEFDSPLIVPDPVDPKRNASSAVSLKSLATFALAS
CCHHHHHHHHHHCCCCEEEEEECCHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KIYLEKPSMEFFFPSKPGRQTIKGDILLVKVKIEESTVEDIIWGQVWRNVEKLKTLISHE
HHEEECCCCEEECCCCCCCCEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
GYRVIDISAWGDAENITIGIQLESKSIGEYYLNVGPYFYLHNVKNFIEENENVWIGEDGR
CEEEEEEEECCCCCEEEEEEEECCCCCCCEEEECCCEEEHHHHHHHHHCCCCEEECCCCC
LYSIKRRRYTVEEIIKRNLSFKQKFTYELQWLTEEVDDPWINLFLRKTPSWLK
EEEEHHHCCCHHHHHHCCCCCHHHHEEEEEEHHHHHCCHHHHHEEECCCCCCC
>Mature Secondary Structure 
TIEEEVLKRIKPSKEDEEKLRERAQIILDRLKGYNAEIEGSFRKGTWLKGDTDIDIFVF
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCCCEEEEEE
FPKSVGKEYLREKALKELIERFRDLNYKIAYAEHPYLIVYVDDVEIDVVPALSIESGEEA
ECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCEEEEEEEEECCCCCHH
ITAADRTPFHTKYVISHLDEKGRDEVRLLKRFLKGIGVYGAEIKVKGFSGYVTELLIIYY
EEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
GSFKEVLRNASKFRPPVRIELVRPKKEFDSPLIVPDPVDPKRNASSAVSLKSLATFALAS
CCHHHHHHHHHHCCCCEEEEEECCHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KIYLEKPSMEFFFPSKPGRQTIKGDILLVKVKIEESTVEDIIWGQVWRNVEKLKTLISHE
HHEEECCCCEEECCCCCCCCEECCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
GYRVIDISAWGDAENITIGIQLESKSIGEYYLNVGPYFYLHNVKNFIEENENVWIGEDGR
CEEEEEEEECCCCCEEEEEEEECCCCCCCEEEECCCEEEHHHHHHHHHCCCCEEECCCCC
LYSIKRRRYTVEEIIKRNLSFKQKFTYELQWLTEEVDDPWINLFLRKTPSWLK
EEEEHHHCCCHHHHHHCCCCCHHHHEEEEEEHHHHHCCHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479