Click here to switch to the map view.

The map label for this gene is eif2a

Identifier: 15921175

GI number: 15921175

Start: 916761

End: 917552

Strand: Reverse

Name: eif2a

Synonym: ST0941

Alternate gene names: 15921175

Gene position: 917552-916761 (Counterclockwise)

Preceding gene: 15921176

Following gene: 15921174

Centisome position: 34.05

GC content: 32.2

Gene sequence:

>792_bases
ATGATTTATAATAAAAACCCTATTCCAACTGAAGGAGAAATTTTAATTGCAACTGTAAAACAAATTTTTGATTATGGTAG
CTATGTAACTCTAGATGAATATGGAAATCTTCAAGCCTTCTTACCTTGGAGCGAAATTAGTAGTAGATGGGTAAAGAACA
TTCGAGATGTGATAAAAGAAGGTAGAAAGATAGTTGTGAAAGTAATTAGAGTGGATAAAAGAAAAGGAACTATAGATGTA
TCTTTGAAAAAAGTTACAGAAGATGAAAAAAGAAAGAAAATGTCACAGTGGAAGAAAATACAAAAAGTTGATAAAATTCT
AGAGATAGTTTCTCAGAAAATAAATAAGACAGAAAAGGAAGGATGGGAACAAGTAGCATGGAAACTTGAAGACAAATACG
GCGATGCTTACGATGCATTAGTTAAGGCAGTTAAAGAGGGAGACTCCATACTTAAAAATGCTGGTGTTCCCGAAGTCTGG
ATTAAACCTCTAATGGAAGAAATTGGGAAACATATAGAAGAAAAAAGAGTTAAAAAAAGTGAAATAGTTACTCTAAGAAG
CAGTGACCCAGCTGGAATTGAAAAAATAAGAAAAGTCTTAGGAGCAGCAGTCGAAATAGCTGAAAATGCTGATGTTGACA
TTAAAATTTACACTATTGGTGCTCCTAGATATAGGATAGATATCATAGGCACAGATCCGAAGCTTCTCTCTAAAGTAATG
ACCGAAATATTAGATAATATAAGAGAAATAAGTAAAGAGGAAAAAGTAGAATTTAATGTGGTAAGAAAATGA

Upstream 100 bases:

>100_bases
CTTTTCCAGTGAGATGCTTATCTTGTGGCACACAACTTGTATATCCTAGGGGCGGTAAGGCTAAAATAGTCGGTGAAACA
GTTAGAATACTAGGTTAAAA

Downstream 100 bases:

>100_bases
AATGGAAAATAAAAAAATGCCCTAAGGATTGCACATACACTTTAAAAGATATATGCCCTATTTGTGGTACTCCTACAATT
ATTCCCCATCCAGCTAGATT

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MIYNKNPIPTEGEILIATVKQIFDYGSYVTLDEYGNLQAFLPWSEISSRWVKNIRDVIKEGRKIVVKVIRVDKRKGTIDV
SLKKVTEDEKRKKMSQWKKIQKVDKILEIVSQKINKTEKEGWEQVAWKLEDKYGDAYDALVKAVKEGDSILKNAGVPEVW
IKPLMEEIGKHIEEKRVKKSEIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPRYRIDIIGTDPKLLSKVM
TEILDNIREISKEEKVEFNVVRK

Sequences:

>Translated_263_residues
MIYNKNPIPTEGEILIATVKQIFDYGSYVTLDEYGNLQAFLPWSEISSRWVKNIRDVIKEGRKIVVKVIRVDKRKGTIDV
SLKKVTEDEKRKKMSQWKKIQKVDKILEIVSQKINKTEKEGWEQVAWKLEDKYGDAYDALVKAVKEGDSILKNAGVPEVW
IKPLMEEIGKHIEEKRVKKSEIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPRYRIDIIGTDPKLLSKVM
TEILDNIREISKEEKVEFNVVRK
>Mature_263_residues
MIYNKNPIPTEGEILIATVKQIFDYGSYVTLDEYGNLQAFLPWSEISSRWVKNIRDVIKEGRKIVVKVIRVDKRKGTIDV
SLKKVTEDEKRKKMSQWKKIQKVDKILEIVSQKINKTEKEGWEQVAWKLEDKYGDAYDALVKAVKEGDSILKNAGVPEVW
IKPLMEEIGKHIEEKRVKKSEIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPRYRIDIIGTDPKLLSKVM
TEILDNIREISKEEKVEFNVVRK

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI4758256, Length=266, Percent_Identity=28.9473684210526, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17509871, Length=265, Percent_Identity=28.6792452830189, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322466, Length=226, Percent_Identity=28.3185840707965, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18860087, Length=267, Percent_Identity=25.0936329588015, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): IF2A_SULTO (Q973G0)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376844.1
- ProteinModelPortal:   Q973G0
- SMR:   Q973G0
- GeneID:   1458904
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0941
- NMPDR:   fig|273063.1.peg.1012
- HOGENOM:   HBG496806
- OMA:   EYPGKEG
- ProtClustDB:   PRK03987
- BioCyc:   STOK273063:ST0941-MONOMER
- HAMAP:   MF_00231
- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30223; Mature: 30223

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYNKNPIPTEGEILIATVKQIFDYGSYVTLDEYGNLQAFLPWSEISSRWVKNIRDVIKE
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHH
GRKIVVKVIRVDKRKGTIDVSLKKVTEDEKRKKMSQWKKIQKVDKILEIVSQKINKTEKE
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GWEQVAWKLEDKYGDAYDALVKAVKEGDSILKNAGVPEVWIKPLMEEIGKHIEEKRVKKS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPRYRIDIIGTDPKLLSKVM
HEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHH
TEILDNIREISKEEKVEFNVVRK
HHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MIYNKNPIPTEGEILIATVKQIFDYGSYVTLDEYGNLQAFLPWSEISSRWVKNIRDVIKE
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHH
GRKIVVKVIRVDKRKGTIDVSLKKVTEDEKRKKMSQWKKIQKVDKILEIVSQKINKTEKE
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GWEQVAWKLEDKYGDAYDALVKAVKEGDSILKNAGVPEVWIKPLMEEIGKHIEEKRVKKS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIVTLRSSDPAGIEKIRKVLGAAVEIAENADVDIKIYTIGAPRYRIDIIGTDPKLLSKVM
HEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHH
TEILDNIREISKEEKVEFNVVRK
HHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479