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The map label for this gene is glgX [H]

Identifier: 15921162

GI number: 15921162

Start: 901072

End: 903222

Strand: Direct

Name: glgX [H]

Synonym: ST0928

Alternate gene names: 15921162

Gene position: 901072-903222 (Clockwise)

Preceding gene: 15921161

Following gene: 15921163

Centisome position: 33.44

GC content: 35.8

Gene sequence:

>2151_bases
ATGGTTTTTTCACACAAGGATAGACCATTAAGACCAGGAGAGCCATATCCTCTTGGAGCTAATTGGGAAGAAGAAGATGA
TGGTGTGAACTTCTCTATCTTTTCGGAAAATGCGACTAAGGTTGAACTTTTAATTTACTCCCCTACTAATCAGAAATATC
CTAAAGAAGTTATCGAGGTTAAGCAGAGATCTGGTGATATTTGGCACGTCTTTGTCCCAGGTTTGGGACCGGGTACACTT
TACGCATATAGAATTTATGGTCCTTATAAGCCAGATCAAGGTTTAAGATTTAATCCTAATAAGGTTCTAATTGATCCTTA
TGCTAAGGCTATAAATGGGACATTAAACTGGAATGATGCTGTTTTTGGTTATAAGATAGGCGATTCTAACCAGGATTTGT
CCTTTGATGATAGGCCAGATGATGAATTTATTCCTAAGGGTGTTGTTATTAATCCTTATTTTGAGTGGGATGATGATCAC
TTTTTTAGGAGAAAGAAGATACCATTAAAGGATACTATTATTTATGAAGTTCATGTTAAAGGTTTTACTAAATTAAGACC
TGATTTACCAGAAAATATTAGAGGTACTTATAAAGGATTTGCCTCTAGACAGATGATCGAATATTTGAAAGATTTGGGGG
TAACTACAGTCGAGATAATGCCAGTACAGCAGTTTGTTGATGATAGGTTTCTAGTAGAGAAGGGATTAAGGAATTACTGG
GGATATAATCCCATAAATTATTTTTCACCTGAATGTAGATATTCCTCTTCTGGCTGTATGGGTGAACAAGTTAACGAGTT
TAAGGAGATGGTTAATGAGCTGCACAACGCTGGCTTCGAGGTAATTATTGATGTTGTTTATAACCATACTGCGGAAGGGA
ATCATTTAGGTCCTACTCTTTCATTCAGAGGTATAGATAATTTGGCTTATTACATGTTAGTTCCAGATAATAAGAGATAT
TATTTAGACTTTACTGGAACTGGAAACACCTTAAATCTGAGTCATCCGAGGGTATTGCAAATGGTTCTGGATAGTCTTAG
ATATTGGGTTTTAGAGATGCATGTTGACGGTTTTAGGTTTGATTTAGCTGCTGCCCTAGCTAGACAATTATACAGTGTAA
ATATGCTTTCAACTTTCTTTGTTGCAATTCAGCAAGATCCCGTTCTTTCTCAAGTTAAGTTAATAGCGGAACCTTGGGAT
GTTGGTCCAGGGGGATATCAGGTTGGTAATTTTCCATATTTGTGGGCCGAATGGAACGGTAAGTATAGAGATACTATAAG
GAGATTTTGGAGAGGTGAGGCGATCCCCTATGAGGAGTTGGCTAATAGGCTTATGGGTTCTCCAGATTTATATGCTGGAA
ATAATAAGACTCCTTTCGCTAGTATAAATTATATAACTTCTCATGATGGTTTTACTTTAGAGGATTTAGTTAGTTATAAT
CAAAAGCATAATGAAGCTAACGGTTTTAATAATCAAGATGGCATGAACGAGAATTATAGTTGGAATTGTGGAGTTGAGGG
AGAGACTAATGATGCTAATGTTATTCAATGTAGAGAGAAACAAAAAAGGAATTTTATCATAACACTTTTTGTAAGTCAAG
GGGTTCCAATGATTTTAGGTGGCGATGAGCTAAGTAGAACACAAAGAGGAAATAACAATGCTTTTTGCCAAGATAACGAA
ATAAGTTGGTTTAATTGGAATCTTGATGAGAGGAAACAGAGGTTTCATGATTTTGTTAGGAGTATGATTTATTTCTATAG
AGCTCATCCAATATTTAGAAGAGAAAGATACTTTCAAGGTAAGAAATTACATGGTATGCCATTAAAGGATGTCACTTTTC
TAAAACCAGATGGAAATGAAGCTGACGAACAAACATGGAAGTCACCAACTAATTTTATTGCATATATTTTAGAGGGTAGT
GTTATTGATGAAGTAAATGATAGGGGTGAGAGAATAGCTGACGATTCTTTCTTAATCATCCTTAATGGTTCACCAAATAA
TATTAAGTTCAAATTCCCGCAAGGTAAATGGAGTTTAGTTGTTTCTTCATATTTGAGAGAACTTAGAGATGACGAGAGAG
TTGTTGATGGTGGCAAGGAACTGGAAATTGAGGGAAGGACCGCAATGGTATATAGGAGGATTGAATATTAG

Upstream 100 bases:

>100_bases
GAATAGTTAGTAGTAAGGAGATTAAGGTAAAGAAAGGTGTTGGTGTTTATAAGTACCTATTGTAAGTAAAGTTTTTATGA
CAATCTTATAAAAATAAACT

Downstream 100 bases:

>100_bases
ATGAAGTTACTTTCAACCTATAGGCTTCAACCAATGAAGTTTTCCGAAATTAGAAATAGGCTGGATTATTTTGTTGAACT
TGGAGTTACTCATCTTTATT

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MVFSHKDRPLRPGEPYPLGANWEEEDDGVNFSIFSENATKVELLIYSPTNQKYPKEVIEVKQRSGDIWHVFVPGLGPGTL
YAYRIYGPYKPDQGLRFNPNKVLIDPYAKAINGTLNWNDAVFGYKIGDSNQDLSFDDRPDDEFIPKGVVINPYFEWDDDH
FFRRKKIPLKDTIIYEVHVKGFTKLRPDLPENIRGTYKGFASRQMIEYLKDLGVTTVEIMPVQQFVDDRFLVEKGLRNYW
GYNPINYFSPECRYSSSGCMGEQVNEFKEMVNELHNAGFEVIIDVVYNHTAEGNHLGPTLSFRGIDNLAYYMLVPDNKRY
YLDFTGTGNTLNLSHPRVLQMVLDSLRYWVLEMHVDGFRFDLAAALARQLYSVNMLSTFFVAIQQDPVLSQVKLIAEPWD
VGPGGYQVGNFPYLWAEWNGKYRDTIRRFWRGEAIPYEELANRLMGSPDLYAGNNKTPFASINYITSHDGFTLEDLVSYN
QKHNEANGFNNQDGMNENYSWNCGVEGETNDANVIQCREKQKRNFIITLFVSQGVPMILGGDELSRTQRGNNNAFCQDNE
ISWFNWNLDERKQRFHDFVRSMIYFYRAHPIFRRERYFQGKKLHGMPLKDVTFLKPDGNEADEQTWKSPTNFIAYILEGS
VIDEVNDRGERIADDSFLIILNGSPNNIKFKFPQGKWSLVVSSYLRELRDDERVVDGGKELEIEGRTAMVYRRIEY

Sequences:

>Translated_716_residues
MVFSHKDRPLRPGEPYPLGANWEEEDDGVNFSIFSENATKVELLIYSPTNQKYPKEVIEVKQRSGDIWHVFVPGLGPGTL
YAYRIYGPYKPDQGLRFNPNKVLIDPYAKAINGTLNWNDAVFGYKIGDSNQDLSFDDRPDDEFIPKGVVINPYFEWDDDH
FFRRKKIPLKDTIIYEVHVKGFTKLRPDLPENIRGTYKGFASRQMIEYLKDLGVTTVEIMPVQQFVDDRFLVEKGLRNYW
GYNPINYFSPECRYSSSGCMGEQVNEFKEMVNELHNAGFEVIIDVVYNHTAEGNHLGPTLSFRGIDNLAYYMLVPDNKRY
YLDFTGTGNTLNLSHPRVLQMVLDSLRYWVLEMHVDGFRFDLAAALARQLYSVNMLSTFFVAIQQDPVLSQVKLIAEPWD
VGPGGYQVGNFPYLWAEWNGKYRDTIRRFWRGEAIPYEELANRLMGSPDLYAGNNKTPFASINYITSHDGFTLEDLVSYN
QKHNEANGFNNQDGMNENYSWNCGVEGETNDANVIQCREKQKRNFIITLFVSQGVPMILGGDELSRTQRGNNNAFCQDNE
ISWFNWNLDERKQRFHDFVRSMIYFYRAHPIFRRERYFQGKKLHGMPLKDVTFLKPDGNEADEQTWKSPTNFIAYILEGS
VIDEVNDRGERIADDSFLIILNGSPNNIKFKFPQGKWSLVVSSYLRELRDDERVVDGGKELEIEGRTAMVYRRIEY
>Mature_716_residues
MVFSHKDRPLRPGEPYPLGANWEEEDDGVNFSIFSENATKVELLIYSPTNQKYPKEVIEVKQRSGDIWHVFVPGLGPGTL
YAYRIYGPYKPDQGLRFNPNKVLIDPYAKAINGTLNWNDAVFGYKIGDSNQDLSFDDRPDDEFIPKGVVINPYFEWDDDH
FFRRKKIPLKDTIIYEVHVKGFTKLRPDLPENIRGTYKGFASRQMIEYLKDLGVTTVEIMPVQQFVDDRFLVEKGLRNYW
GYNPINYFSPECRYSSSGCMGEQVNEFKEMVNELHNAGFEVIIDVVYNHTAEGNHLGPTLSFRGIDNLAYYMLVPDNKRY
YLDFTGTGNTLNLSHPRVLQMVLDSLRYWVLEMHVDGFRFDLAAALARQLYSVNMLSTFFVAIQQDPVLSQVKLIAEPWD
VGPGGYQVGNFPYLWAEWNGKYRDTIRRFWRGEAIPYEELANRLMGSPDLYAGNNKTPFASINYITSHDGFTLEDLVSYN
QKHNEANGFNNQDGMNENYSWNCGVEGETNDANVIQCREKQKRNFIITLFVSQGVPMILGGDELSRTQRGNNNAFCQDNE
ISWFNWNLDERKQRFHDFVRSMIYFYRAHPIFRRERYFQGKKLHGMPLKDVTFLKPDGNEADEQTWKSPTNFIAYILEGS
VIDEVNDRGERIADDSFLIILNGSPNNIKFKFPQGKWSLVVSSYLRELRDDERVVDGGKELEIEGRTAMVYRRIEY

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=671, Percent_Identity=41.1326378539493, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83054; Mature: 83054

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSHKDRPLRPGEPYPLGANWEEEDDGVNFSIFSENATKVELLIYSPTNQKYPKEVIEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
KQRSGDIWHVFVPGLGPGTLYAYRIYGPYKPDQGLRFNPNKVLIDPYAKAINGTLNWNDA
HHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCCEEEECCHHHHHCCCCCCCCC
VFGYKIGDSNQDLSFDDRPDDEFIPKGVVINPYFEWDDDHFFRRKKIPLKDTIIYEVHVK
EEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCHHHHHCCCCCCCEEEEEEEEC
GFTKLRPDLPENIRGTYKGFASRQMIEYLKDLGVTTVEIMPVQQFVDDRFLVEKGLRNYW
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHC
GYNPINYFSPECRYSSSGCMGEQVNEFKEMVNELHNAGFEVIIDVVYNHTAEGNHLGPTL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCEE
SFRGIDNLAYYMLVPDNKRYYLDFTGTGNTLNLSHPRVLQMVLDSLRYWVLEMHVDGFRF
EECCCCCEEEEEEEECCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHEEEEEECCCHHH
DLAAALARQLYSVNMLSTFFVAIQQDPVLSQVKLIAEPWDVGPGGYQVGNFPYLWAEWNG
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEECCC
KYRDTIRRFWRGEAIPYEELANRLMGSPDLYAGNNKTPFASINYITSHDGFTLEDLVSYN
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCCCCCEEEEEEEECCCCCCHHHHHHHH
QKHNEANGFNNQDGMNENYSWNCGVEGETNDANVIQCREKQKRNFIITLFVSQGVPMILG
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECHHCCCCEEEEEEECCCCCEEEC
GDELSRTQRGNNNAFCQDNEISWFNWNLDERKQRFHDFVRSMIYFYRAHPIFRRERYFQG
CCHHHHHHCCCCCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KKLHGMPLKDVTFLKPDGNEADEQTWKSPTNFIAYILEGSVIDEVNDRGERIADDSFLII
CCCCCCCCCCEEEECCCCCCCHHHHHCCHHHEEEEECCCCHHHHHHHCCCEECCCCEEEE
LNGSPNNIKFKFPQGKWSLVVSSYLRELRDDERVVDGGKELEIEGRTAMVYRRIEY
EECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEEEEECC
>Mature Secondary Structure
MVFSHKDRPLRPGEPYPLGANWEEEDDGVNFSIFSENATKVELLIYSPTNQKYPKEVIEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
KQRSGDIWHVFVPGLGPGTLYAYRIYGPYKPDQGLRFNPNKVLIDPYAKAINGTLNWNDA
HHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCCEEEECCHHHHHCCCCCCCCC
VFGYKIGDSNQDLSFDDRPDDEFIPKGVVINPYFEWDDDHFFRRKKIPLKDTIIYEVHVK
EEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEEECCCCHHHHHCCCCCCCEEEEEEEEC
GFTKLRPDLPENIRGTYKGFASRQMIEYLKDLGVTTVEIMPVQQFVDDRFLVEKGLRNYW
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHC
GYNPINYFSPECRYSSSGCMGEQVNEFKEMVNELHNAGFEVIIDVVYNHTAEGNHLGPTL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCEE
SFRGIDNLAYYMLVPDNKRYYLDFTGTGNTLNLSHPRVLQMVLDSLRYWVLEMHVDGFRF
EECCCCCEEEEEEEECCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHEEEEEECCCHHH
DLAAALARQLYSVNMLSTFFVAIQQDPVLSQVKLIAEPWDVGPGGYQVGNFPYLWAEWNG
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCEEEEECCC
KYRDTIRRFWRGEAIPYEELANRLMGSPDLYAGNNKTPFASINYITSHDGFTLEDLVSYN
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCCCCCEEEEEEEECCCCCCHHHHHHHH
QKHNEANGFNNQDGMNENYSWNCGVEGETNDANVIQCREKQKRNFIITLFVSQGVPMILG
HCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEECHHCCCCEEEEEEECCCCCEEEC
GDELSRTQRGNNNAFCQDNEISWFNWNLDERKQRFHDFVRSMIYFYRAHPIFRRERYFQG
CCHHHHHHCCCCCCCEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KKLHGMPLKDVTFLKPDGNEADEQTWKSPTNFIAYILEGSVIDEVNDRGERIADDSFLII
CCCCCCCCCCEEEECCCCCCCHHHHHCCHHHEEEEECCCCHHHHHHHCCCEECCCCEEEE
LNGSPNNIKFKFPQGKWSLVVSSYLRELRDDERVVDGGKELEIEGRTAMVYRRIEY
EECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]