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The map label for this gene is ascB [C]

Identifier: 15921007

GI number: 15921007

Start: 752547

End: 753701

Strand: Reverse

Name: ascB [C]

Synonym: ST0773

Alternate gene names: 15921007

Gene position: 753701-752547 (Counterclockwise)

Preceding gene: 15921008

Following gene: 15921005

Centisome position: 27.97

GC content: 34.55

Gene sequence:

>1155_bases
ATGAAATTAGGTTTCTCGATTTCAGCATTCCAGTACGAGGAACTGAATGAGAATTCAGATTGGTATTTGTGGTTAACAGA
TGAGATTAACATAACAAGTAAGCGAGTAATAGGAGAATTACCAGGTAAGAATTTCTATCTCTCTAAGTTTTCTCAAATTC
ACGATATAGCTTCTAGGCTTAACGCATCATTCTGGAGACTGAACTTAAGTTGGGGAAGGATATTTAAGGAAAGAGATAAA
ATCTCTGTAGAAGCTGTAACTGGATATAGAAAATTACTCAAAGACTTGAAAGATAGAGGATTTAAGGTTATATTATGTCT
TAATCATTTCGATCTTCCTAAGTGGGTTCACGATCCAATTATTGCTAGAGATTCTCTCTTAACTGAAGGACCTTTAGGTT
GGTATTCAGAAGATACGATTAATCACTTTATATCTTTTTCGTCTTTTGTGAAAGATAATTTTTCCGAATACGTAGATCTT
TGGTGTACCTTTAACGAACCTAATATAATGATACTTTTTGGTTATTTATCTGGGATTTTCCCACCTGGGATAACGAGCAG
AAGAGCATATGAAAAAGCCTTGAAAAATGTCTTAACTGCACATCGAGAGGTTTATAACCTATTTCACGGAGAGAAAGTAG
GAATCATTTTCAATTTTCCGTATATTCAGGGTAACGAGAAAGCTAAAGAAGAGCTTTTCACAACTTTGAAAGGCATAAGC
TTTGATTGGATTGGTGTCAATTATTATACAAGAATAGTTGTTAATGAGAAAGGACAACCAGTTGACGGTTATGGTATGTT
TTGTAAACCGAATTCTTTTTCATTAGATAATAATCCTTGTTCCGATTACGGCTGGGAAGTTTACCCAGAGGGGCTAAAGC
ATGTCTTACAAGGCGTGAAAAAATTCGATAAACCAATTATAGTAACTGAGAATGGTATTGCTGATTCCAAAGATTTCCTT
AGACCAAGTTTCTTAATATCGCATGTGGAAGCTATAAAGGAAAGTAAAGTTAATGTTGAAGCCTATCTATACTGGAGTTT
AATTGATAATTTTGAATGGAATTTCGGCTATCAAATGAAGTTCGGGATTTACACACTCGACTTGAAGGCAAGACCTAGCG
CTTATATCTTCAAAGAATTAACTTCTTTTGTCTGA

Upstream 100 bases:

>100_bases
ATGTAGAACTTTCCTTAATATTTAGATTTAGAGAAAAATGGACTGAAGAACATTTTAAGAGCGGTACTATTCATGCTTTA
GAGAGGATAGTGAGTGAAAA

Downstream 100 bases:

>100_bases
TAATTCTTTATCCATATCTTCTATAATTGTAATTATAACTGGTGACAAATATCTGAGAATTTCTACGTGCTTTATCAAAA
AAATGACTCCTTTATCATTT

Product: beta-galactosidase

Products: NA

Alternate protein names: Lactase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKLGFSISAFQYEELNENSDWYLWLTDEINITSKRVIGELPGKNFYLSKFSQIHDIASRLNASFWRLNLSWGRIFKERDK
ISVEAVTGYRKLLKDLKDRGFKVILCLNHFDLPKWVHDPIIARDSLLTEGPLGWYSEDTINHFISFSSFVKDNFSEYVDL
WCTFNEPNIMILFGYLSGIFPPGITSRRAYEKALKNVLTAHREVYNLFHGEKVGIIFNFPYIQGNEKAKEELFTTLKGIS
FDWIGVNYYTRIVVNEKGQPVDGYGMFCKPNSFSLDNNPCSDYGWEVYPEGLKHVLQGVKKFDKPIIVTENGIADSKDFL
RPSFLISHVEAIKESKVNVEAYLYWSLIDNFEWNFGYQMKFGIYTLDLKARPSAYIFKELTSFV

Sequences:

>Translated_384_residues
MKLGFSISAFQYEELNENSDWYLWLTDEINITSKRVIGELPGKNFYLSKFSQIHDIASRLNASFWRLNLSWGRIFKERDK
ISVEAVTGYRKLLKDLKDRGFKVILCLNHFDLPKWVHDPIIARDSLLTEGPLGWYSEDTINHFISFSSFVKDNFSEYVDL
WCTFNEPNIMILFGYLSGIFPPGITSRRAYEKALKNVLTAHREVYNLFHGEKVGIIFNFPYIQGNEKAKEELFTTLKGIS
FDWIGVNYYTRIVVNEKGQPVDGYGMFCKPNSFSLDNNPCSDYGWEVYPEGLKHVLQGVKKFDKPIIVTENGIADSKDFL
RPSFLISHVEAIKESKVNVEAYLYWSLIDNFEWNFGYQMKFGIYTLDLKARPSAYIFKELTSFV
>Mature_384_residues
MKLGFSISAFQYEELNENSDWYLWLTDEINITSKRVIGELPGKNFYLSKFSQIHDIASRLNASFWRLNLSWGRIFKERDK
ISVEAVTGYRKLLKDLKDRGFKVILCLNHFDLPKWVHDPIIARDSLLTEGPLGWYSEDTINHFISFSSFVKDNFSEYVDL
WCTFNEPNIMILFGYLSGIFPPGITSRRAYEKALKNVLTAHREVYNLFHGEKVGIIFNFPYIQGNEKAKEELFTTLKGIS
FDWIGVNYYTRIVVNEKGQPVDGYGMFCKPNSFSLDNNPCSDYGWEVYPEGLKHVLQGVKKFDKPIIVTENGIADSKDFL
RPSFLISHVEAIKESKVNVEAYLYWSLIDNFEWNFGYQMKFGIYTLDLKARPSAYIFKELTSFV

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=200, Percent_Identity=30, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI13273313, Length=166, Percent_Identity=30.1204819277108, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI24497614, Length=358, Percent_Identity=23.1843575418994, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17552856, Length=427, Percent_Identity=25.0585480093677, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539390, Length=421, Percent_Identity=23.2779097387173, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356577, Length=418, Percent_Identity=24.4019138755981, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 44674; Mature: 44674

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGFSISAFQYEELNENSDWYLWLTDEINITSKRVIGELPGKNFYLSKFSQIHDIASRL
CCCCCEEEEEEHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHC
NASFWRLNLSWGRIFKERDKISVEAVTGYRKLLKDLKDRGFKVILCLNHFDLPKWVHDPI
CCEEEEEECHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCH
IARDSLLTEGPLGWYSEDTINHFISFSSFVKDNFSEYVDLWCTFNEPNIMILFGYLSGIF
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEHHHCCC
PPGITSRRAYEKALKNVLTAHREVYNLFHGEKVGIIFNFPYIQGNEKAKEELFTTLKGIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHHCCE
FDWIGVNYYTRIVVNEKGQPVDGYGMFCKPNSFSLDNNPCSDYGWEVYPEGLKHVLQGVK
EEEEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
KFDKPIIVTENGIADSKDFLRPSFLISHVEAIKESKVNVEAYLYWSLIDNFEWNFGYQMK
HHCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEE
FGIYTLDLKARPSAYIFKELTSFV
EEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLGFSISAFQYEELNENSDWYLWLTDEINITSKRVIGELPGKNFYLSKFSQIHDIASRL
CCCCCEEEEEEHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHC
NASFWRLNLSWGRIFKERDKISVEAVTGYRKLLKDLKDRGFKVILCLNHFDLPKWVHDPI
CCEEEEEECHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCH
IARDSLLTEGPLGWYSEDTINHFISFSSFVKDNFSEYVDLWCTFNEPNIMILFGYLSGIF
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEHHHCCC
PPGITSRRAYEKALKNVLTAHREVYNLFHGEKVGIIFNFPYIQGNEKAKEELFTTLKGIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHHCCE
FDWIGVNYYTRIVVNEKGQPVDGYGMFCKPNSFSLDNNPCSDYGWEVYPEGLKHVLQGVK
EEEEEEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
KFDKPIIVTENGIADSKDFLRPSFLISHVEAIKESKVNVEAYLYWSLIDNFEWNFGYQMK
HHCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEE
FGIYTLDLKARPSAYIFKELTSFV
EEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10833397 [H]