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The map label for this gene is nfsA [C]

Identifier: 15920926

GI number: 15920926

Start: 681710

End: 682264

Strand: Reverse

Name: nfsA [C]

Synonym: ST0697

Alternate gene names: 15920926

Gene position: 682264-681710 (Counterclockwise)

Preceding gene: 15920928

Following gene: 15920925

Centisome position: 25.32

GC content: 34.41

Gene sequence:

>555_bases
ATGAACTTGAAATATAACAATATGAACGATATTCTTGAGTTTCTAATAAAGAGAAGAAGTGTGAGAAGTTTTCAGCAAAA
GCCCGTAGATTTAGAGTTAATAAAACAATTAATATATGTAGCAAACCATGCACCAAACTCAATGAATAATGAGCCTTGGA
AATTCATTATTATTATAGATGAGGAGATAAAAAAGAAGCTTTCCACGCTACATAAAGGAGCTTCACACATAGCTTTAGCA
CCTATAGGTGTAGCTGTAGTGGCTGAACCAGAAATAAGTCCAGAAACTTGGATGGTCGATGTAGTAAATGCTACTATGTA
TTTTGTATTAGCAGCACATTCAGTAGGCTTAGGAGTTGGATGGATAGCAGCTTATGAAAATAAAAAGGCTAAAGAGATCT
TAGGAATACCAGATAATAAAGTCCTAGTTACATTACTTTCTGTCGGCTATCCAGATCCTAACTATAAGCCTAGAGAAAAG
AGTGTGAAAAATCCGGAGAGTGTGATGTTCATAAATAAGTGGGGAAATATGCTAATTAGGATTAAGGAGAATTAA

Upstream 100 bases:

>100_bases
TACTGATATCATATTTAGGTTAATTTCGTCAAGCCTGTATGTATAATTCATATTTAAGCATTTGATTTTCAGTTTAAAAC
TCTTTAGTTAAATTCTTAAG

Downstream 100 bases:

>100_bases
GATTTATGCGAATAAAAGATGCTATTATCAAAATTTTTTACACTCGTAAGGATGTAAGTGAGATTTACTTAGTAATTAGA
GATAGGGTTAAGGGAACAAG

Product: NADH dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNLKYNNMNDILEFLIKRRSVRSFQQKPVDLELIKQLIYVANHAPNSMNNEPWKFIIIIDEEIKKKLSTLHKGASHIALA
PIGVAVVAEPEISPETWMVDVVNATMYFVLAAHSVGLGVGWIAAYENKKAKEILGIPDNKVLVTLLSVGYPDPNYKPREK
SVKNPESVMFINKWGNMLIRIKEN

Sequences:

>Translated_184_residues
MNLKYNNMNDILEFLIKRRSVRSFQQKPVDLELIKQLIYVANHAPNSMNNEPWKFIIIIDEEIKKKLSTLHKGASHIALA
PIGVAVVAEPEISPETWMVDVVNATMYFVLAAHSVGLGVGWIAAYENKKAKEILGIPDNKVLVTLLSVGYPDPNYKPREK
SVKNPESVMFINKWGNMLIRIKEN
>Mature_184_residues
MNLKYNNMNDILEFLIKRRSVRSFQQKPVDLELIKQLIYVANHAPNSMNNEPWKFIIIIDEEIKKKLSTLHKGASHIALA
PIGVAVVAEPEISPETWMVDVVNATMYFVLAAHSVGLGVGWIAAYENKKAKEILGIPDNKVLVTLLSVGYPDPNYKPREK
SVKNPESVMFINKWGNMLIRIKEN

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20869; Mature: 20869

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKYNNMNDILEFLIKRRSVRSFQQKPVDLELIKQLIYVANHAPNSMNNEPWKFIIIID
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
EEIKKKLSTLHKGASHIALAPIGVAVVAEPEISPETWMVDVVNATMYFVLAAHSVGLGVG
HHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCE
WIAAYENKKAKEILGIPDNKVLVTLLSVGYPDPNYKPREKSVKNPESVMFINKWGNMLIR
EEEECCCCCHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
IKEN
EECC
>Mature Secondary Structure
MNLKYNNMNDILEFLIKRRSVRSFQQKPVDLELIKQLIYVANHAPNSMNNEPWKFIIIID
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
EEIKKKLSTLHKGASHIALAPIGVAVVAEPEISPETWMVDVVNATMYFVLAAHSVGLGVG
HHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCE
WIAAYENKKAKEILGIPDNKVLVTLLSVGYPDPNYKPREKSVKNPESVMFINKWGNMLIR
EEEECCCCCHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
IKEN
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]