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The map label for this gene is pdhD [H]

Identifier: 15920912

GI number: 15920912

Start: 670196

End: 671563

Strand: Direct

Name: pdhD [H]

Synonym: ST0687

Alternate gene names: 15920912

Gene position: 670196-671563 (Clockwise)

Preceding gene: 15920911

Following gene: 15920914

Centisome position: 24.87

GC content: 33.7

Gene sequence:

>1368_bases
ATGAAATATGATGTAACTGTTATTGGTGCTGGAGGCGGAGGTTACCCTGGTGCATTCAGATTAGCTAAATCTGGATATAA
TGTATTAATGGCTGACCCTAAAGGAGAATTAGGGGGAAATTGTCTTTATAGTGGCTGTGTCCCATCAAAGACAGTAAGAG
AAATGGCACAATTAATTTGGAGAACAAGTAGAGTATTAAAGCAAGAAATTAAAGTGAATTTTGAAAGAATACAAGCTCAT
AAAGATTTTGTTCAAGAGACAAGATTTAAACAACATAAAAGAGAACTTTCTGAGTATAATAGTATAACATTTTATAAAGG
AGTAATTAAGATAAAAGATCCAAATCATGTAATTGTAAAGACTGAAGATAAAGAAATAGAAGCAGAAACACGTTATATAA
TTATTGCCTCAGGTAGTGAACCGTTTAAACCACAATTCCCTGGAAGTGAGTATTGCATAACAAGTGATGATTTATATAGT
TATAAAACTCCACTAAGAAAATTACCACAAGATATGGTTATAATTGGTGGTGGATATATTGCTATTGAAACAGCCTCAAT
CTTTAATATTTTAGGTGTTAAAACTCATTTGTTAGTTAGAGGTGATAGGGTTCTAAGAGGCTTTGAAGACGAAATAGTTA
ATACCCTGTTACCCATTTTAAAACTCGATATAATTTATAACGCACCGGTTCTTGAAATAAAGAAAATTAAGGAAGATGAA
TTTGAAGTCATATATAGTAGTAAAGATGGTGCAAAGAAAAGTATTAGAACAAATTTGGTCCTACTAGCTACTGGCAGAAA
ACCAGTATTGCCAGAAGGGATAGAAAACACTGGAATTGCTTTAGATAAAAGAGGATATATAGTTGTAGATAATGCAATGA
GGACTAATCTTCCAAACGTTTTTGCTACTGGTGACGTTAATGGTAAAGCACCGTACTTTCATGCGGCAGTCAGAATGAGC
ATTGCCGCAGCTTATAACATTATGGCAAACGGTTCTCCTATAGACTATGTAGATTACAAAAGCATACCAGTTACAATTTA
TTCAGTTCCTTCAGCTTCTTATGTAGGAATAATGCCAAGTGAGGCTAAAAGAATGGGAATAGAGATTATGGAGGCAACAT
ATAATATGGAAGATGAAGTCATGGCACAAATGTATGATGAAAGAGAAGGTATGTTAAAACTAATCTTTGAAAAAGGTAGC
CTAAGATTAATAGGTGCTTGGATGGTTGGTGTACATTCTCAATATTTAATTAATGAACTTGGACAAGCGGTACTTCATGG
TTTAACTGCAAAACAGTTAGCAAGTTTTGCAGACCAACACCCTTCAACAAATGAGATTATTGCATATGCTGCAAGAAAAG
TGTTATAA

Upstream 100 bases:

>100_bases
AACAAATAGTCGACTTACTTAAGTAAGAAATTCTTCATTTCCCTCTAGTTACTATAAACTATTCGAAAGCTTTATAGATT
AGTAATTAAAGAAAAGTCTT

Downstream 100 bases:

>100_bases
CATTATTTTTTATCTCATAGTTTTCTCAGAACTGAAAAATAAGGTACTATGGCAATATATATTTATTCTTAGTTAAGTTT
GTAAATAAGAATTAACTCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKYDVTVIGAGGGGYPGAFRLAKSGYNVLMADPKGELGGNCLYSGCVPSKTVREMAQLIWRTSRVLKQEIKVNFERIQAH
KDFVQETRFKQHKRELSEYNSITFYKGVIKIKDPNHVIVKTEDKEIEAETRYIIIASGSEPFKPQFPGSEYCITSDDLYS
YKTPLRKLPQDMVIIGGGYIAIETASIFNILGVKTHLLVRGDRVLRGFEDEIVNTLLPILKLDIIYNAPVLEIKKIKEDE
FEVIYSSKDGAKKSIRTNLVLLATGRKPVLPEGIENTGIALDKRGYIVVDNAMRTNLPNVFATGDVNGKAPYFHAAVRMS
IAAAYNIMANGSPIDYVDYKSIPVTIYSVPSASYVGIMPSEAKRMGIEIMEATYNMEDEVMAQMYDEREGMLKLIFEKGS
LRLIGAWMVGVHSQYLINELGQAVLHGLTAKQLASFADQHPSTNEIIAYAARKVL

Sequences:

>Translated_455_residues
MKYDVTVIGAGGGGYPGAFRLAKSGYNVLMADPKGELGGNCLYSGCVPSKTVREMAQLIWRTSRVLKQEIKVNFERIQAH
KDFVQETRFKQHKRELSEYNSITFYKGVIKIKDPNHVIVKTEDKEIEAETRYIIIASGSEPFKPQFPGSEYCITSDDLYS
YKTPLRKLPQDMVIIGGGYIAIETASIFNILGVKTHLLVRGDRVLRGFEDEIVNTLLPILKLDIIYNAPVLEIKKIKEDE
FEVIYSSKDGAKKSIRTNLVLLATGRKPVLPEGIENTGIALDKRGYIVVDNAMRTNLPNVFATGDVNGKAPYFHAAVRMS
IAAAYNIMANGSPIDYVDYKSIPVTIYSVPSASYVGIMPSEAKRMGIEIMEATYNMEDEVMAQMYDEREGMLKLIFEKGS
LRLIGAWMVGVHSQYLINELGQAVLHGLTAKQLASFADQHPSTNEIIAYAARKVL
>Mature_455_residues
MKYDVTVIGAGGGGYPGAFRLAKSGYNVLMADPKGELGGNCLYSGCVPSKTVREMAQLIWRTSRVLKQEIKVNFERIQAH
KDFVQETRFKQHKRELSEYNSITFYKGVIKIKDPNHVIVKTEDKEIEAETRYIIIASGSEPFKPQFPGSEYCITSDDLYS
YKTPLRKLPQDMVIIGGGYIAIETASIFNILGVKTHLLVRGDRVLRGFEDEIVNTLLPILKLDIIYNAPVLEIKKIKEDE
FEVIYSSKDGAKKSIRTNLVLLATGRKPVLPEGIENTGIALDKRGYIVVDNAMRTNLPNVFATGDVNGKAPYFHAAVRMS
IAAAYNIMANGSPIDYVDYKSIPVTIYSVPSASYVGIMPSEAKRMGIEIMEATYNMEDEVMAQMYDEREGMLKLIFEKGS
LRLIGAWMVGVHSQYLINELGQAVLHGLTAKQLASFADQHPSTNEIIAYAARKVL

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=29.4759825327511, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=28.6315789473684, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI33519430, Length=479, Percent_Identity=26.5135699373695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519428, Length=479, Percent_Identity=26.5135699373695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519426, Length=479, Percent_Identity=26.5135699373695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277065, Length=479, Percent_Identity=26.5135699373695, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277071, Length=479, Percent_Identity=26.5135699373695, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI291045266, Length=453, Percent_Identity=24.5033112582781, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI22035672, Length=462, Percent_Identity=23.1601731601732, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI291045268, Length=305, Percent_Identity=26.8852459016393, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=29.6536796536797, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=27.0454545454545, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=27.6548672566372, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082354, Length=439, Percent_Identity=26.879271070615, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=27.6688453159041, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=24.8447204968944, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=440, Percent_Identity=25.6818181818182, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=440, Percent_Identity=25.6818181818182, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=445, Percent_Identity=24.0449438202247, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=27.5423728813559, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=27.9220779220779, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=26.2054507337526, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21358499, Length=461, Percent_Identity=26.8980477223427, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=26.8343815513627, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=26.9473684210526, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=26.9473684210526, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=25.2083333333333, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50702; Mature: 50702

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDVTVIGAGGGGYPGAFRLAKSGYNVLMADPKGELGGNCLYSGCVPSKTVREMAQLIW
CCEEEEEEECCCCCCCHHHHEECCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHH
RTSRVLKQEIKVNFERIQAHKDFVQETRFKQHKRELSEYNSITFYKGVIKIKDPNHVIVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEE
TEDKEIEAETRYIIIASGSEPFKPQFPGSEYCITSDDLYSYKTPLRKLPQDMVIIGGGYI
ECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHCHHHHCCCCEEEECCCEE
AIETASIFNILGVKTHLLVRGDRVLRGFEDEIVNTLLPILKLDIIYNAPVLEIKKIKEDE
EEEHHHHHHHHCCEEEEEEECHHHHHCHHHHHHHHHHHHHHHHHEECCCCEEHHHCCCCC
FEVIYSSKDGAKKSIRTNLVLLATGRKPVLPEGIENTGIALDKRGYIVVDNAMRTNLPNV
EEEEEECCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEECCHHHCCCCCE
FATGDVNGKAPYFHAAVRMSIAAAYNIMANGSPIDYVDYKSIPVTIYSVPSASYVGIMPS
EEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCEEEEECCCCCEEECCCC
EAKRMGIEIMEATYNMEDEVMAQMYDEREGMLKLIFEKGSLRLIGAWMVGVHSQYLINEL
HHHHHCCEEEEHHCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHH
GQAVLHGLTAKQLASFADQHPSTNEIIAYAARKVL
HHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKYDVTVIGAGGGGYPGAFRLAKSGYNVLMADPKGELGGNCLYSGCVPSKTVREMAQLIW
CCEEEEEEECCCCCCCHHHHEECCCCEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHH
RTSRVLKQEIKVNFERIQAHKDFVQETRFKQHKRELSEYNSITFYKGVIKIKDPNHVIVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCEEEEE
TEDKEIEAETRYIIIASGSEPFKPQFPGSEYCITSDDLYSYKTPLRKLPQDMVIIGGGYI
ECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHCHHHHCCCCEEEECCCEE
AIETASIFNILGVKTHLLVRGDRVLRGFEDEIVNTLLPILKLDIIYNAPVLEIKKIKEDE
EEEHHHHHHHHCCEEEEEEECHHHHHCHHHHHHHHHHHHHHHHHEECCCCEEHHHCCCCC
FEVIYSSKDGAKKSIRTNLVLLATGRKPVLPEGIENTGIALDKRGYIVVDNAMRTNLPNV
EEEEEECCCCCCHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEECCHHHCCCCCE
FATGDVNGKAPYFHAAVRMSIAAAYNIMANGSPIDYVDYKSIPVTIYSVPSASYVGIMPS
EEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCEEEEECCCCCEEECCCC
EAKRMGIEIMEATYNMEDEVMAQMYDEREGMLKLIFEKGSLRLIGAWMVGVHSQYLINEL
HHHHHCCEEEEHHCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHH
GQAVLHGLTAKQLASFADQHPSTNEIIAYAARKVL
HHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]