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The map label for this gene is garR [C]

Identifier: 15920839

GI number: 15920839

Start: 603694

End: 604569

Strand: Direct

Name: garR [C]

Synonym: ST0619

Alternate gene names: 15920839

Gene position: 603694-604569 (Clockwise)

Preceding gene: 15920838

Following gene: 15920841

Centisome position: 22.4

GC content: 34.7

Gene sequence:

>876_bases
ATGCGTGTTGGCTTAGCTGGTTTAGGTGTTATGGGGTATAGGATAGGTGCAAACTTAGTAAAGGCTGGTAAACTAGATGT
AGTATATAACAGAACTGTTAGTAGGGCGGAACAGTTTAGTAAGGAATATGGTGTTAAATACGTAACTGACCCTAAAGAGT
TAATTAAATCTGTAGATTTGTTAATAACAATGTTAGCTGACGATAGTGCAGTTTCTTCATTCCTATTGCCTTTATCCCCT
TATGCTAAAGATAAAATAATTGTTGACATGTCTACAATATCTCCTTCAACTTCAATTAGTATTGCTAATGAAATAATGAA
GAATGGTGGTATGATGTATGATGCCCCAGTAATTGGTACTTCAATATTTGCAGAACAGAAGAAATTGACTGTATTACTAG
GCGGGCCAGAATCTCATGTAAATACTGTTACGGAAATTTTGAAGGAAACAGCTTCCACGATAATTTACATGGGTAAGAAT
GGGATGGGTCTTTATGCAAAGTTAGTTAATAATCTTATGGTTGGTGTATATGTAGCAGCTTTAGCTGAGGCTTATAATTT
TGGAATTTCTGCTGGATTGAAACCAGAGGATGTTCATAAAGTTTTAGCATTATATGGTAGTGCTAAATCTCCAACCTCAG
AGTTAAAAGTTCCAAAGATGATGAACTCAGATTATTCAACTCAATTTGCAACTAAACATATGAGAAAAGACTTAGAAATT
ATAACCAAGGAAACGCAGAATTTGCACGTTGTTAATCCGTTATCATCTTTAGCACTTCAGCTTTATCGTTTTGCTGAAGC
TTTAGGATATTCAGAATCTGATTATGCAGCTGTTCTTGAGGTATACAAGAAGGCTAACTTAGTTAAAGTTAAGTAA

Upstream 100 bases:

>100_bases
TGAAATTAATAAGAAGGGACAATGTATTGAAGCAATTTAAAATAAAAGAGTAGAATGTTTGATAACAAACTTTATTTTTT
ATTTTTACTACCTCCTTATT

Downstream 100 bases:

>100_bases
GGGCTAGGATATCTTCTTTTCCATTAATTTTCTTTCTACCTCTAAATCCTTCTTCTGCGTAATGCCAGAATTCAATGTCT
TTTTCATCAATTTTCCAGCA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRVGLAGLGVMGYRIGANLVKAGKLDVVYNRTVSRAEQFSKEYGVKYVTDPKELIKSVDLLITMLADDSAVSSFLLPLSP
YAKDKIIVDMSTISPSTSISIANEIMKNGGMMYDAPVIGTSIFAEQKKLTVLLGGPESHVNTVTEILKETASTIIYMGKN
GMGLYAKLVNNLMVGVYVAALAEAYNFGISAGLKPEDVHKVLALYGSAKSPTSELKVPKMMNSDYSTQFATKHMRKDLEI
ITKETQNLHVVNPLSSLALQLYRFAEALGYSESDYAAVLEVYKKANLVKVK

Sequences:

>Translated_291_residues
MRVGLAGLGVMGYRIGANLVKAGKLDVVYNRTVSRAEQFSKEYGVKYVTDPKELIKSVDLLITMLADDSAVSSFLLPLSP
YAKDKIIVDMSTISPSTSISIANEIMKNGGMMYDAPVIGTSIFAEQKKLTVLLGGPESHVNTVTEILKETASTIIYMGKN
GMGLYAKLVNNLMVGVYVAALAEAYNFGISAGLKPEDVHKVLALYGSAKSPTSELKVPKMMNSDYSTQFATKHMRKDLEI
ITKETQNLHVVNPLSSLALQLYRFAEALGYSESDYAAVLEVYKKANLVKVK
>Mature_291_residues
MRVGLAGLGVMGYRIGANLVKAGKLDVVYNRTVSRAEQFSKEYGVKYVTDPKELIKSVDLLITMLADDSAVSSFLLPLSP
YAKDKIIVDMSTISPSTSISIANEIMKNGGMMYDAPVIGTSIFAEQKKLTVLLGGPESHVNTVTEILKETASTIIYMGKN
GMGLYAKLVNNLMVGVYVAALAEAYNFGISAGLKPEDVHKVLALYGSAKSPTSELKVPKMMNSDYSTQFATKHMRKDLEI
ITKETQNLHVVNPLSSLALQLYRFAEALGYSESDYAAVLEVYKKANLVKVK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=31.8493150684932, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=25.6140350877193, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=27.891156462585, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786719, Length=287, Percent_Identity=27.1777003484321, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=29.1095890410959, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789092, Length=297, Percent_Identity=26.9360269360269, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557316, Length=306, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=30.4794520547945, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=30.4794520547945, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=27.4021352313167, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31682; Mature: 31682

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGLAGLGVMGYRIGANLVKAGKLDVVYNRTVSRAEQFSKEYGVKYVTDPKELIKSVDL
CCCCCHHHHHHHHHHCCHHHCCCCEEEEEHHHHHHHHHHHHHHCCEEECCHHHHHHHHHH
LITMLADDSAVSSFLLPLSPYAKDKIIVDMSTISPSTSISIANEIMKNGGMMYDAPVIGT
HHHHHCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHH
SIFAEQKKLTVLLGGPESHVNTVTEILKETASTIIYMGKNGMGLYAKLVNNLMVGVYVAA
HHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
LAEAYNFGISAGLKPEDVHKVLALYGSAKSPTSELKVPKMMNSDYSTQFATKHMRKDLEI
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHH
ITKETQNLHVVNPLSSLALQLYRFAEALGYSESDYAAVLEVYKKANLVKVK
HHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MRVGLAGLGVMGYRIGANLVKAGKLDVVYNRTVSRAEQFSKEYGVKYVTDPKELIKSVDL
CCCCCHHHHHHHHHHCCHHHCCCCEEEEEHHHHHHHHHHHHHHCCEEECCHHHHHHHHHH
LITMLADDSAVSSFLLPLSPYAKDKIIVDMSTISPSTSISIANEIMKNGGMMYDAPVIGT
HHHHHCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHH
SIFAEQKKLTVLLGGPESHVNTVTEILKETASTIIYMGKNGMGLYAKLVNNLMVGVYVAA
HHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
LAEAYNFGISAGLKPEDVHKVLALYGSAKSPTSELKVPKMMNSDYSTQFATKHMRKDLEI
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHH
ITKETQNLHVVNPLSSLALQLYRFAEALGYSESDYAAVLEVYKKANLVKVK
HHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA