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The map label for this gene is hflB [C]

Identifier: 15920793

GI number: 15920793

Start: 564452

End: 565810

Strand: Direct

Name: hflB [C]

Synonym: ST0575

Alternate gene names: 15920793

Gene position: 564452-565810 (Clockwise)

Preceding gene: 15920790

Following gene: 15920795

Centisome position: 20.95

GC content: 35.47

Gene sequence:

>1359_bases
ATGGCAGAGATTAGAGAGATAAGAAAGATTGAACGCGAAAGAGCAAGTATTCATAGTCATATTTCTGGATTAGGTTTAGA
TGAAAAAGGAAAAGCCAAATTTAAGGCTGATGGTCTAGTTGGGCAAACAGAAGCACGTGAAGCTGCAGGTATTGTCGTTC
AACTTATCAAACAAGGTAAAATGGCCGGAAAAGGGGTACTATTCGTAGGCCCTCCTGGAACTGGAAAAACGGCCTTAGCT
GTTGCAATAGCCAAAGAGCTTGGAGAAGACACACCCTTTACTACAATGAATGCTTCTGAAGTTTATTCGACAGAGTTAAA
GAAGACAGAAATACTCACACAAGCTATTAGAAAATCAATAGGCGTTAGGATTAAGCAAAGGAGAATTGTTTATGAAGGTG
TAGTAAAGGATGTAAAATTAAAAGTAGCAAGAAGTAGATTAAATCCTTATGCTGTTATGCCTAGAGAAGCACAAATAGTT
CTAGCTACTAAGGATGAGGAGAAAACACTAAATGTGGGAGATAGCATTGCTGAACAACTAGTTCAACTTAATGTAAAAAA
GGGAGATGTAATATGGATCGACGCTCAAACTGGAGAGGTAAGTAAAGTTGGTAAGGCTAAGGGGTTTGAAGGAGCTAAAA
CTTATGATATAGAAACTATTAGACAAGTTGATATTCCTACTGGCCCGGTTAAGAAGGAAAAAGAAACCACAATAACTGTA
ACATTACATGATTTAGATTTAAACGTAGCAGCTAGAAATATTTCTATAACAGCGTTATTTAGTTTCTTTACAGAAAGAGA
AATAAACAGTGAGATAAGAGAAAGTGTAGATAAATTAGTAAAAGATATGATAAATAGGGGAGAAGCTGAACTCGTACCTG
GTGTATTATTTATTGATGATGCACATATGTTAGATATAGAAGCATTCTCATTCTTAACTAAAGCATTAGAAGCTGACCTA
GCACCAATATTAATTTTAGCTACTAATAGAGGTATAACAAAAATAAGGGGTACCGACATAGAATCTCCACACGGTATGCC
ACTAGATCTACTCGATAGATTACTAATAATACCTACAAGACCATATACTGCTGATGAAATAAGAGAAATTCTAAAAATAA
GAGCAGACGAAATTGATGTTCATCTAGAAGAGAAAGCGTTAGAGACTTTAACGAAGCTAGGCGTAGAATATAGTTTAAGA
TATTCCGTCCAGCTCCTTGAGCCTTCTTATGTAATTGCACAAAGAAACGGTAGAAATGTGATAAAAGAAGAAGATGTACT
AGAAGCATCGAAATTGTTCTCAGATTTCAGAAGAAGTGTGGAATATGTTAAGGAATACGAGAAATTACTACTAAAATGA

Upstream 100 bases:

>100_bases
CATATCAGTACCAAAGGTCTTCATCACTACATATAATTTTCATGTTAGTAACTTAAAAGGATAATCTCAAAAGCTTATAG
TAATATATAATATAACGAAT

Downstream 100 bases:

>100_bases
TGAAAGGCAACGGCACTGAATTATGATGTATTGTTGCCTGGCTGAATACATTTTTCGTATATTTTTTTGTCTATAATATC
CCTTAATATTCTATATGTCA

Product: TATA-binding protein-interacting protein

Products: NA

Alternate protein names: TIP49 Domain Protein; TIP49-Like Protein; TIP49 Domain-Containing Protein; Tbp-Interacting Protein Tip; DNA Helicase; TIP49-Like; TBP-Interacting Proten; TIP49 C-Terminal Domain Family Protein; DNA Helicase TIP

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAEIREIRKIERERASIHSHISGLGLDEKGKAKFKADGLVGQTEAREAAGIVVQLIKQGKMAGKGVLFVGPPGTGKTALA
VAIAKELGEDTPFTTMNASEVYSTELKKTEILTQAIRKSIGVRIKQRRIVYEGVVKDVKLKVARSRLNPYAVMPREAQIV
LATKDEEKTLNVGDSIAEQLVQLNVKKGDVIWIDAQTGEVSKVGKAKGFEGAKTYDIETIRQVDIPTGPVKKEKETTITV
TLHDLDLNVAARNISITALFSFFTEREINSEIRESVDKLVKDMINRGEAELVPGVLFIDDAHMLDIEAFSFLTKALEADL
APILILATNRGITKIRGTDIESPHGMPLDLLDRLLIIPTRPYTADEIREILKIRADEIDVHLEEKALETLTKLGVEYSLR
YSVQLLEPSYVIAQRNGRNVIKEEDVLEASKLFSDFRRSVEYVKEYEKLLLK

Sequences:

>Translated_452_residues
MAEIREIRKIERERASIHSHISGLGLDEKGKAKFKADGLVGQTEAREAAGIVVQLIKQGKMAGKGVLFVGPPGTGKTALA
VAIAKELGEDTPFTTMNASEVYSTELKKTEILTQAIRKSIGVRIKQRRIVYEGVVKDVKLKVARSRLNPYAVMPREAQIV
LATKDEEKTLNVGDSIAEQLVQLNVKKGDVIWIDAQTGEVSKVGKAKGFEGAKTYDIETIRQVDIPTGPVKKEKETTITV
TLHDLDLNVAARNISITALFSFFTEREINSEIRESVDKLVKDMINRGEAELVPGVLFIDDAHMLDIEAFSFLTKALEADL
APILILATNRGITKIRGTDIESPHGMPLDLLDRLLIIPTRPYTADEIREILKIRADEIDVHLEEKALETLTKLGVEYSLR
YSVQLLEPSYVIAQRNGRNVIKEEDVLEASKLFSDFRRSVEYVKEYEKLLLK
>Mature_451_residues
AEIREIRKIERERASIHSHISGLGLDEKGKAKFKADGLVGQTEAREAAGIVVQLIKQGKMAGKGVLFVGPPGTGKTALAV
AIAKELGEDTPFTTMNASEVYSTELKKTEILTQAIRKSIGVRIKQRRIVYEGVVKDVKLKVARSRLNPYAVMPREAQIVL
ATKDEEKTLNVGDSIAEQLVQLNVKKGDVIWIDAQTGEVSKVGKAKGFEGAKTYDIETIRQVDIPTGPVKKEKETTITVT
LHDLDLNVAARNISITALFSFFTEREINSEIRESVDKLVKDMINRGEAELVPGVLFIDDAHMLDIEAFSFLTKALEADLA
PILILATNRGITKIRGTDIESPHGMPLDLLDRLLIIPTRPYTADEIREILKIRADEIDVHLEEKALETLTKLGVEYSLRY
SVQLLEPSYVIAQRNGRNVIKEEDVLEASKLFSDFRRSVEYVKEYEKLLLK

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG1224

COG function: function code K; DNA helicase TIP49, TBP-interacting protein

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI5730023, Length=451, Percent_Identity=45.4545454545455, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI4506753, Length=457, Percent_Identity=45.7330415754923, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17542510, Length=438, Percent_Identity=44.2922374429224, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17558290, Length=454, Percent_Identity=40.0881057268723, Blast_Score=316, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6325021, Length=432, Percent_Identity=45.3703703703704, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320396, Length=432, Percent_Identity=46.2962962962963, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17737635, Length=447, Percent_Identity=44.9664429530201, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI21358125, Length=450, Percent_Identity=46.2222222222222, Blast_Score=349, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.24.- [C]

Molecular weight: Translated: 50357; Mature: 50226

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIREIRKIERERASIHSHISGLGLDEKGKAKFKADGLVGQTEAREAAGIVVQLIKQGK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
MAGKGVLFVGPPGTGKTALAVAIAKELGEDTPFTTMNASEVYSTELKKTEILTQAIRKSI
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHH
GVRIKQRRIVYEGVVKDVKLKVARSRLNPYAVMPREAQIVLATKDEEKTLNVGDSIAEQL
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
VQLNVKKGDVIWIDAQTGEVSKVGKAKGFEGAKTYDIETIRQVDIPTGPVKKEKETTITV
HHEECCCCCEEEEECCCCCHHHHHCCCCCCCCCEECHHHHEEECCCCCCCCCCCCCEEEE
TLHDLDLNVAARNISITALFSFFTEREINSEIRESVDKLVKDMINRGEAELVPGVLFIDD
EEEECEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
AHMLDIEAFSFLTKALEADLAPILILATNRGITKIRGTDIESPHGMPLDLLDRLLIIPTR
CCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEECCC
PYTADEIREILKIRADEIDVHLEEKALETLTKLGVEYSLRYSVQLLEPSYVIAQRNGRNV
CCCHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCCC
IKEEDVLEASKLFSDFRRSVEYVKEYEKLLLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEIREIRKIERERASIHSHISGLGLDEKGKAKFKADGLVGQTEAREAAGIVVQLIKQGK
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
MAGKGVLFVGPPGTGKTALAVAIAKELGEDTPFTTMNASEVYSTELKKTEILTQAIRKSI
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHH
GVRIKQRRIVYEGVVKDVKLKVARSRLNPYAVMPREAQIVLATKDEEKTLNVGDSIAEQL
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
VQLNVKKGDVIWIDAQTGEVSKVGKAKGFEGAKTYDIETIRQVDIPTGPVKKEKETTITV
HHEECCCCCEEEEECCCCCHHHHHCCCCCCCCCEECHHHHEEECCCCCCCCCCCCCEEEE
TLHDLDLNVAARNISITALFSFFTEREINSEIRESVDKLVKDMINRGEAELVPGVLFIDD
EEEECEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
AHMLDIEAFSFLTKALEADLAPILILATNRGITKIRGTDIESPHGMPLDLLDRLLIIPTR
CCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHHCCEEEECCC
PYTADEIREILKIRADEIDVHLEEKALETLTKLGVEYSLRYSVQLLEPSYVIAQRNGRNV
CCCHHHHHHHHHHCCCHHEEEEHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCCC
IKEEDVLEASKLFSDFRRSVEYVKEYEKLLLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA