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The map label for this gene is mptN [H]

Identifier: 15920703

GI number: 15920703

Start: 487037

End: 487942

Strand: Direct

Name: mptN [H]

Synonym: ST0488

Alternate gene names: 15920703

Gene position: 487037-487942 (Clockwise)

Preceding gene: 15920702

Following gene: 15920707

Centisome position: 18.07

GC content: 32.89

Gene sequence:

>906_bases
GTGTTCACTTGGTGGATTGATATTAGAATAGCAGTTATTCATGAATCTCAAAAAATTACAGAGTCAGCTAAGCAACTTCT
ACTAGAGATAAAGAACACAGGGAATACGCCAATATACATTAGAATTTCCAAATTAAATCCTATAATAACTAAGGATGGTT
TAGAATTTAGTTATGGAGGAAAAAGAATAGATTTAGATGGTGCAGTAATACGGAACTTAGGGTTTATATCTTCAACAGAG
CAGTTAATGAAAAGATTTGATACTCTCAAAGCCCTTGAAAGTAGTGGTGTTACATTAATAAATTCACCGGACTCCATGTT
TATCGCAAGAGATAAGTTTAACAGTTTATTAAAACTAAAGATGGCTAAAGTACCAGTTCCAGATACTACTATAGTAGAAG
ATCCCTTTGAGGTGATGAGACTTGTTAATGAATGGAAAGATGTTGTAATAAAACCCGTTATAGGTAGTCTAGGACTTGGT
TCAGTACGAGTTTCTGATCCAGATATAGCGTTTAGAGTGGCAAAAGCAATACTATCTGTTAATCAACCAGTTTATGTTCA
GAAATATGTTGATAAGCCAGAAAGAGATATAAGAGTGTTTGTTGTAGGTAACTCAATACTAGGAAGTGTATATAGAATTA
ACAAATCTTCATGGAAAACTAATGTAGCTCAAGGGTCTCTTACTCAAGTTCTCTTACCAAATCATGAACTTGAAGAATTG
TCACTTAAAGCAGTTAAAGCATTGAAATTAGATTATGCAGGGATAGATATCGTAGAAGATAAGGATGGCAGTTATAAGGT
TATCGAAGTAAATGCTGCTCCTCTTTGGAAAGGATTTTACGAAGCTACACATATTAATCCTGCAAAATATATAATTGAAT
ATCTCATTAAAAAAATTAGGAAATGA

Upstream 100 bases:

>100_bases
TCAAGATTTTACTGAAGCTATAATATCGTTGCCAACTTTTTTAACGAAGGGGCTTTTAAACGAAGCCGGGCTGGATAAAT
TATAAAAAGTCTTCTATCGA

Downstream 100 bases:

>100_bases
AGAATAATAAACCGGCTGAAAACATGATGAAGAGCTTTCGCACGGATTAAGTAAAGTCTATTGTCTTCATTTCTTTAATT
GTTCTTAATACAAACATGGT

Product: hypothetical protein

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MFTWWIDIRIAVIHESQKITESAKQLLLEIKNTGNTPIYIRISKLNPIITKDGLEFSYGGKRIDLDGAVIRNLGFISSTE
QLMKRFDTLKALESSGVTLINSPDSMFIARDKFNSLLKLKMAKVPVPDTTIVEDPFEVMRLVNEWKDVVIKPVIGSLGLG
SVRVSDPDIAFRVAKAILSVNQPVYVQKYVDKPERDIRVFVVGNSILGSVYRINKSSWKTNVAQGSLTQVLLPNHELEEL
SLKAVKALKLDYAGIDIVEDKDGSYKVIEVNAAPLWKGFYEATHINPAKYIIEYLIKKIRK

Sequences:

>Translated_301_residues
MFTWWIDIRIAVIHESQKITESAKQLLLEIKNTGNTPIYIRISKLNPIITKDGLEFSYGGKRIDLDGAVIRNLGFISSTE
QLMKRFDTLKALESSGVTLINSPDSMFIARDKFNSLLKLKMAKVPVPDTTIVEDPFEVMRLVNEWKDVVIKPVIGSLGLG
SVRVSDPDIAFRVAKAILSVNQPVYVQKYVDKPERDIRVFVVGNSILGSVYRINKSSWKTNVAQGSLTQVLLPNHELEEL
SLKAVKALKLDYAGIDIVEDKDGSYKVIEVNAAPLWKGFYEATHINPAKYIIEYLIKKIRK
>Mature_301_residues
MFTWWIDIRIAVIHESQKITESAKQLLLEIKNTGNTPIYIRISKLNPIITKDGLEFSYGGKRIDLDGAVIRNLGFISSTE
QLMKRFDTLKALESSGVTLINSPDSMFIARDKFNSLLKLKMAKVPVPDTTIVEDPFEVMRLVNEWKDVVIKPVIGSLGLG
SVRVSDPDIAFRVAKAILSVNQPVYVQKYVDKPERDIRVFVVGNSILGSVYRINKSSWKTNVAQGSLTQVLLPNHELEEL
SLKAVKALKLDYAGIDIVEDKDGSYKVIEVNAAPLWKGFYEATHINPAKYIIEYLIKKIRK

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=212, Percent_Identity=28.7735849056604, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33921; Mature: 33921

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTWWIDIRIAVIHESQKITESAKQLLLEIKNTGNTPIYIRISKLNPIITKDGLEFSYGG
CEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEECCCC
KRIDLDGAVIRNLGFISSTEQLMKRFDTLKALESSGVTLINSPDSMFIARDKFNSLLKLK
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHH
MAKVPVPDTTIVEDPFEVMRLVNEWKDVVIKPVIGSLGLGSVRVSDPDIAFRVAKAILSV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHC
NQPVYVQKYVDKPERDIRVFVVGNSILGSVYRINKSSWKTNVAQGSLTQVLLPNHELEEL
CCCEEEEHHHCCCCCCEEEEEECCHHHHHEEEECCCCCCCCCCCCCEEEEECCCCCHHHH
SLKAVKALKLDYAGIDIVEDKDGSYKVIEVNAAPLWKGFYEATHINPAKYIIEYLIKKIR
HHHHHHHHEEECCCEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MFTWWIDIRIAVIHESQKITESAKQLLLEIKNTGNTPIYIRISKLNPIITKDGLEFSYGG
CEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCCEECCCC
KRIDLDGAVIRNLGFISSTEQLMKRFDTLKALESSGVTLINSPDSMFIARDKFNSLLKLK
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHH
MAKVPVPDTTIVEDPFEVMRLVNEWKDVVIKPVIGSLGLGSVRVSDPDIAFRVAKAILSV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHEEHHHHCCCCCCEEEECCCHHHHHHHHHHHHC
NQPVYVQKYVDKPERDIRVFVVGNSILGSVYRINKSSWKTNVAQGSLTQVLLPNHELEEL
CCCEEEEHHHCCCCCCEEEEEECCHHHHHEEEECCCCCCCCCCCCCEEEEECCCCCHHHH
SLKAVKALKLDYAGIDIVEDKDGSYKVIEVNAAPLWKGFYEATHINPAKYIIEYLIKKIR
HHHHHHHHEEECCCEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]