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The map label for this gene is prf1 [H]

Identifier: 15920698

GI number: 15920698

Start: 482856

End: 483878

Strand: Direct

Name: prf1 [H]

Synonym: ST0483

Alternate gene names: 15920698

Gene position: 482856-483878 (Clockwise)

Preceding gene: 15920697

Following gene: 15920700

Centisome position: 17.92

GC content: 33.82

Gene sequence:

>1023_bases
GTGTTGCTATCATTGTACATACCACCTGGAAGACCTATACCAGATGTTCTCAATTTATTAAGGCAAGAATATTCAATAGC
GCAAAATATTAAATTGAAGAGAACTAGGGATGCAGTAACTTCGGCAATCCAATCAGCTATAGATAGACTAACGCAAATAC
CTAAGGTTCCAGATAACGGATTAGTTATATTCTGTGGTGAGAATTTCGAGACAGAAGAATCAAAATGTTTTGTGTTCTCT
CCACCGGAAAAAGTTACAATATTCTTTTACAGAACAGACAAATATTTTCATACTGAATTTCTAGAAGATATGATAGAAGA
AAATGACGTTTTCGGATTAATAATAGTTGAAAGAGATTATGCTACAATTGGTATATTAAAAGGAACCAGAATAGAAGTGC
TTGACGAGTTTGAAGGCTTTGTACCGGGTAAGCATATGATGGGTGGACAGTCTCAAAGACGTATTGATAGAATAATTGAG
GAAATGTATGCTAATTTCTTGAAAGAAGTAGGAGAAAAGGCAAATTCATATTTACTTCCTTATTTAGAGAGCGGGAAATT
AAAGGGAATATTATTAGGAGGACCAGGATATGCAAAGAAAGATTTCTACGATGGAGATTATTTAGATTATAGGCTAAAAA
AATTGGTTCTATTACCCCTTATTGATATTCCGGACCAAGGTGATGCTGGATTAAAGGAGTTAGTTATGAAGGCACAAGAT
ATTTTGAAAAACCAGAAATATGTCCAAGTTCAAAATCTCCTAGAAGAACTAAAATATCATATCGCTAAGGATGACGGCCT
AGTAGTTTATGGTATTGAAGAAATAAGAAAAGCGTTACATATGGGTGCTGTCGATTCATTAGTTATCTATGATGAGCCAA
ATAGTGAATTAGAGAAACTTTCACAAGAAGCAGAAAATTATGGTACAAAAGTTTATGTAGTAGGGGATGAATTGCCAGAT
GCTGAATGGGTCAAAAAGACATTTGGTGGAGCTATAGGTAAATTAAGATATAAAATATACTAA

Upstream 100 bases:

>100_bases
ATATATCGATAAGGGATGAGTCTTTGATAAATATCAAATAACTAAACAAGAATTAAAAGTTCTTATAAGAGAATTGAAAA
AATGGTCTGCCCCAGCTACA

Downstream 100 bases:

>100_bases
AGTAGATTTCTTCTTTTCATTTCAGTCAGAATTTTTTCTACTGGTATAGGTGGTTCTACACCATAACTTTCTATAAACTT
TGCTTTTAAATAGGAAGTAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLLSLYIPPGRPIPDVLNLLRQEYSIAQNIKLKRTRDAVTSAIQSAIDRLTQIPKVPDNGLVIFCGENFETEESKCFVFS
PPEKVTIFFYRTDKYFHTEFLEDMIEENDVFGLIIVERDYATIGILKGTRIEVLDEFEGFVPGKHMMGGQSQRRIDRIIE
EMYANFLKEVGEKANSYLLPYLESGKLKGILLGGPGYAKKDFYDGDYLDYRLKKLVLLPLIDIPDQGDAGLKELVMKAQD
ILKNQKYVQVQNLLEELKYHIAKDDGLVVYGIEEIRKALHMGAVDSLVIYDEPNSELEKLSQEAENYGTKVYVVGDELPD
AEWVKKTFGGAIGKLRYKIY

Sequences:

>Translated_340_residues
MLLSLYIPPGRPIPDVLNLLRQEYSIAQNIKLKRTRDAVTSAIQSAIDRLTQIPKVPDNGLVIFCGENFETEESKCFVFS
PPEKVTIFFYRTDKYFHTEFLEDMIEENDVFGLIIVERDYATIGILKGTRIEVLDEFEGFVPGKHMMGGQSQRRIDRIIE
EMYANFLKEVGEKANSYLLPYLESGKLKGILLGGPGYAKKDFYDGDYLDYRLKKLVLLPLIDIPDQGDAGLKELVMKAQD
ILKNQKYVQVQNLLEELKYHIAKDDGLVVYGIEEIRKALHMGAVDSLVIYDEPNSELEKLSQEAENYGTKVYVVGDELPD
AEWVKKTFGGAIGKLRYKIY
>Mature_340_residues
MLLSLYIPPGRPIPDVLNLLRQEYSIAQNIKLKRTRDAVTSAIQSAIDRLTQIPKVPDNGLVIFCGENFETEESKCFVFS
PPEKVTIFFYRTDKYFHTEFLEDMIEENDVFGLIIVERDYATIGILKGTRIEVLDEFEGFVPGKHMMGGQSQRRIDRIIE
EMYANFLKEVGEKANSYLLPYLESGKLKGILLGGPGYAKKDFYDGDYLDYRLKKLVLLPLIDIPDQGDAGLKELVMKAQD
ILKNQKYVQVQNLLEELKYHIAKDDGLVVYGIEEIRKALHMGAVDSLVIYDEPNSELEKLSQEAENYGTKVYVVGDELPD
AEWVKKTFGGAIGKLRYKIY

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=387, Percent_Identity=29.9741602067183, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI72000368, Length=385, Percent_Identity=28.051948051948, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319619, Length=385, Percent_Identity=29.3506493506493, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI28574632, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21356433, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24667392, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24667388, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24667380, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24667384, Length=314, Percent_Identity=32.1656050955414, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 38816; Mature: 38816

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSLYIPPGRPIPDVLNLLRQEYSIAQNIKLKRTRDAVTSAIQSAIDRLTQIPKVPDNG
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVIFCGENFETEESKCFVFSPPEKVTIFFYRTDKYFHTEFLEDMIEENDVFGLIIVERDY
EEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCC
ATIGILKGTRIEVLDEFEGFVPGKHMMGGQSQRRIDRIIEEMYANFLKEVGEKANSYLLP
EEEEEECCCHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
YLESGKLKGILLGGPGYAKKDFYDGDYLDYRLKKLVLLPLIDIPDQGDAGLKELVMKAQD
EECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
ILKNQKYVQVQNLLEELKYHIAKDDGLVVYGIEEIRKALHMGAVDSLVIYDEPNSELEKL
HHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCEEEEEECCCHHHHHH
SQEAENYGTKVYVVGDELPDAEWVKKTFGGAIGKLRYKIY
HHHHHHHCCEEEEECCCCCCHHHHHHHHCCHHHHEEEEEC
>Mature Secondary Structure
MLLSLYIPPGRPIPDVLNLLRQEYSIAQNIKLKRTRDAVTSAIQSAIDRLTQIPKVPDNG
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVIFCGENFETEESKCFVFSPPEKVTIFFYRTDKYFHTEFLEDMIEENDVFGLIIVERDY
EEEEECCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCC
ATIGILKGTRIEVLDEFEGFVPGKHMMGGQSQRRIDRIIEEMYANFLKEVGEKANSYLLP
EEEEEECCCHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
YLESGKLKGILLGGPGYAKKDFYDGDYLDYRLKKLVLLPLIDIPDQGDAGLKELVMKAQD
EECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
ILKNQKYVQVQNLLEELKYHIAKDDGLVVYGIEEIRKALHMGAVDSLVIYDEPNSELEKL
HHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCEEEEEECCCHHHHHH
SQEAENYGTKVYVVGDELPDAEWVKKTFGGAIGKLRYKIY
HHHHHHHCCEEEEECCCCCCHHHHHHHHCCHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]