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The map label for this gene is metE [H]

Identifier: 15920589

GI number: 15920589

Start: 400942

End: 401910

Strand: Reverse

Name: metE [H]

Synonym: ST0386

Alternate gene names: 15920589

Gene position: 401910-400942 (Counterclockwise)

Preceding gene: 15920590

Following gene: 15920588

Centisome position: 14.91

GC content: 27.66

Gene sequence:

>969_bases
ATGAGAATAGAAACAGCACTAATCGGGAGCTATCCTAAACCAGTGAGAATCGCTAAAATAATTTCAAAGAAAAATAATGG
AAAAATATCTGAGGAGAAATATCTGGAGCAAATATTCAATTTTATGAGTAATTTTTTTGAGATGATGAAATCCTTTAATG
TTGATTTTACTACAGACAGTATGATAGAGTGGGATGATATTGCAGACTTAACATATTCTTTCCTTACCAATGTTAAAAAG
GGAAGTCTAACGAGATTTTTTGACAATAACTTCTACTATAGACAAATTGTAATAAATGAAAAACTGAAGTACAAAGAGGA
TAATACATACATTAAATATTTTGAAAAAGCTAAAGAGCTTTCAAAAAGTTATAATTTTAAACTTAAAGCAGTAATTTTAG
GACCATTAACTTTTCTTAAACTTTCTGAAAATCAATACTACAAAAACGAAGAAGAGTTAATGAAGGACTATGCTCTAATA
GTAAACTCACTACTGAAGAACATAAAAAGCGATGTAGTAGAAATTCACGAACCCTCAATTTTCCAAAAAGGAATAAAGAA
GGACTTATTGAACACTCTTCCAGAAATTTACAATACAATGCTAGAAAATATAAACAATGAAATACATTTATTAACTTATT
TTGATATAAATTTTGATAGATTAGAAAATTATATGAAATTGCCAGCAACTATTTATGGATTTGACGTGACTGAAAGCAAT
AAAAATAAGCTTGGTAGGCTGTATCAATTCCTAAAAGGAAAGCAAGTATATTTTGGAATCCTAGATTCAAGGACAACAAA
AATGGAAAAAATTGTTACAATTAAAAGAATAGTTTCTACTGCATCCGAAAAAGGAATAGAAAGGTTAATTTTAGGTAACT
CCTCCTTTAATGATTTTATACCAGAAATAATAGTTCAGAAAAAATTTAAATTATTACAAAAAGCAAAGGAGATGATCTTA
AATGGATGA

Upstream 100 bases:

>100_bases
ACGTCAAGGTAGCATTTGCGGATCAAAAACCTATGCAGTTGGAATTCAACATGGAAGGAGGGGGGAAAGTTACTTATTTG
CTTGCCCCCAAATTATCCTG

Downstream 100 bases:

>100_bases
ATTACCAATTCTTCCAACAACAGTTATAGGTAGTTATCCTAGACCGAAATGGTTAAGAGAAGCAATACGATTACATAAAG
CCGGAAAAATTTCTGATGAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRIETALIGSYPKPVRIAKIISKKNNGKISEEKYLEQIFNFMSNFFEMMKSFNVDFTTDSMIEWDDIADLTYSFLTNVKK
GSLTRFFDNNFYYRQIVINEKLKYKEDNTYIKYFEKAKELSKSYNFKLKAVILGPLTFLKLSENQYYKNEEELMKDYALI
VNSLLKNIKSDVVEIHEPSIFQKGIKKDLLNTLPEIYNTMLENINNEIHLLTYFDINFDRLENYMKLPATIYGFDVTESN
KNKLGRLYQFLKGKQVYFGILDSRTTKMEKIVTIKRIVSTASEKGIERLILGNSSFNDFIPEIIVQKKFKLLQKAKEMIL
NG

Sequences:

>Translated_322_residues
MRIETALIGSYPKPVRIAKIISKKNNGKISEEKYLEQIFNFMSNFFEMMKSFNVDFTTDSMIEWDDIADLTYSFLTNVKK
GSLTRFFDNNFYYRQIVINEKLKYKEDNTYIKYFEKAKELSKSYNFKLKAVILGPLTFLKLSENQYYKNEEELMKDYALI
VNSLLKNIKSDVVEIHEPSIFQKGIKKDLLNTLPEIYNTMLENINNEIHLLTYFDINFDRLENYMKLPATIYGFDVTESN
KNKLGRLYQFLKGKQVYFGILDSRTTKMEKIVTIKRIVSTASEKGIERLILGNSSFNDFIPEIIVQKKFKLLQKAKEMIL
NG
>Mature_322_residues
MRIETALIGSYPKPVRIAKIISKKNNGKISEEKYLEQIFNFMSNFFEMMKSFNVDFTTDSMIEWDDIADLTYSFLTNVKK
GSLTRFFDNNFYYRQIVINEKLKYKEDNTYIKYFEKAKELSKSYNFKLKAVILGPLTFLKLSENQYYKNEEELMKDYALI
VNSLLKNIKSDVVEIHEPSIFQKGIKKDLLNTLPEIYNTMLENINNEIHLLTYFDINFDRLENYMKLPATIYGFDVTESN
KNKLGRLYQFLKGKQVYFGILDSRTTKMEKIVTIKRIVSTASEKGIERLILGNSSFNDFIPEIIVQKKFKLLQKAKEMIL
NG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 37910; Mature: 37910

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIETALIGSYPKPVRIAKIISKKNNGKISEEKYLEQIFNFMSNFFEMMKSFNVDFTTDS
CCCCCHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MIEWDDIADLTYSFLTNVKKGSLTRFFDNNFYYRQIVINEKLKYKEDNTYIKYFEKAKEL
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
SKSYNFKLKAVILGPLTFLKLSENQYYKNEEELMKDYALIVNSLLKNIKSDVVEIHEPSI
HHHCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FQKGIKKDLLNTLPEIYNTMLENINNEIHLLTYFDINFDRLENYMKLPATIYGFDVTESN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCEEEEEECCCCC
KNKLGRLYQFLKGKQVYFGILDSRTTKMEKIVTIKRIVSTASEKGIERLILGNSSFNDFI
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
PEIIVQKKFKLLQKAKEMILNG
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIETALIGSYPKPVRIAKIISKKNNGKISEEKYLEQIFNFMSNFFEMMKSFNVDFTTDS
CCCCCHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MIEWDDIADLTYSFLTNVKKGSLTRFFDNNFYYRQIVINEKLKYKEDNTYIKYFEKAKEL
CCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
SKSYNFKLKAVILGPLTFLKLSENQYYKNEEELMKDYALIVNSLLKNIKSDVVEIHEPSI
HHHCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FQKGIKKDLLNTLPEIYNTMLENINNEIHLLTYFDINFDRLENYMKLPATIYGFDVTESN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCCEEEEEECCCCC
KNKLGRLYQFLKGKQVYFGILDSRTTKMEKIVTIKRIVSTASEKGIERLILGNSSFNDFI
HHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
PEIIVQKKFKLLQKAKEMILNG
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]