Click here to switch to the map view.

The map label for this gene is speE

Identifier: 15920547

GI number: 15920547

Start: 366070

End: 366972

Strand: Direct

Name: speE

Synonym: ST0346

Alternate gene names: 15920547

Gene position: 366070-366972 (Clockwise)

Preceding gene: 15920546

Following gene: 15920548

Centisome position: 13.58

GC content: 31.45

Gene sequence:

>903_bases
ATGTATGAATGGCATTGGCATATAGAATGGCAAACTCCATACGAATTTCATGGACACGCTATAACTAAAGTAATTGCGGA
AGAAAAAACACCTTATCAGAGAGCTCTTTTAGTTGAATTAGCTAGATTCGGGAAAGCACTAATATTAGACGGAAAAATAC
AATCTACAATAACTGACGAGTTCATATACCATGAGGCATTAGTACATCCATTACTTTTATCTATAAATAATCCAGAAAAA
ATTCTAATATTAGGAGGGGGAGAAGGAGCAACATTAAGAGAAGTTCTTAAACATAAAACTATAAAGAATGTTACTATGGT
TGATATAGATCCTGTAGTAATCGATTTTGCCAAAAAATATTTACAAGAATGGCATCAAGGAGCTTTCGATAATCCCAAAA
GTAAATTAGTCATAGAGGATGGCTATAAATTTATTAAAGAAACAAAAGAGAAATTTGACGCAGTAGTTATTGATCTTACA
GACCCAATAAAAGATTCGCCTTCCCAAATGCTATATACTAAAGAGTTTTATGAAGAAGTTAAGAGAATAAGTAAATGGGG
TATAGTAACACAAGCTACTTCTCCATCATTTAGTTTAGAAACCTTCTCCATAATTTATAATACAATAAAACATGTTTTCA
AAAAAGTAAGTGCTGGAATAACATATGTACCGGCATTTGACGGCTTATGGGGATTTGTATATGCTTCAGATGAAGTTAAT
CCAGCAGAATTTAGTAAAGAAGAAATTAATAATAGAATTAAAGAAAGAATAGATGGAAGTCTTAGATTTTATGATGGTGA
AACTCATATTACAATGTTTAGTATCCCGAAACATATAAGGGAAGTACTAGAAAAAGAAAATAAAATTTCGACTAGAGAAA
ATCCAGTAGCAGTTCCAGCATAG

Upstream 100 bases:

>100_bases
TAGAAAAGGCTATTGTGCTGCATGCGGATTTGGAAGAAGTAAGAAAATAAGAAGATATAATTGGCAAAATAAGAAAGTTA
ATGGTTTGAGGCTCGTATAA

Downstream 100 bases:

>100_bases
TCTTTTTATTTTATGTTTTCAAATTTTATACTTGCCGGGGTGCCCGAGCGGACTAAGGGGGTAGGCTCGAGACTTTCCAG
CGTTAAGATGGCGTGCGACC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MYEWHWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPEK
ILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLT
DPIKDSPSQMLYTKEFYEEVKRISKWGIVTQATSPSFSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVN
PAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRENPVAVPA

Sequences:

>Translated_300_residues
MYEWHWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPEK
ILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLT
DPIKDSPSQMLYTKEFYEEVKRISKWGIVTQATSPSFSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVN
PAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRENPVAVPA
>Mature_300_residues
MYEWHWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPEK
ILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLT
DPIKDSPSQMLYTKEFYEEVKRISKWGIVTQATSPSFSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVN
PAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRENPVAVPA

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=267, Percent_Identity=28.8389513108614, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786312, Length=287, Percent_Identity=34.4947735191638, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI212645616, Length=227, Percent_Identity=36.5638766519824, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI25154106, Length=273, Percent_Identity=33.6996336996337, Blast_Score=137, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323175, Length=275, Percent_Identity=31.6363636363636, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325326, Length=267, Percent_Identity=32.2097378277154, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24645443, Length=282, Percent_Identity=35.4609929078014, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21355941, Length=213, Percent_Identity=35.2112676056338, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24663238, Length=159, Percent_Identity=35.2201257861635, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358269, Length=159, Percent_Identity=35.2201257861635, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_SULTO (Q975S5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376216.1
- ProteinModelPortal:   Q975S5
- SMR:   Q975S5
- GeneID:   1458266
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0346
- NMPDR:   fig|273063.1.peg.384
- HOGENOM:   HBG635113
- OMA:   VEGGPCY
- ProtClustDB:   CLSK883670
- BioCyc:   STOK273063:ST0346-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 34576; Mature: 34576

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEWHWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDE
CCEEEEEEEECCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
FIYHEALVHPLLLSINNPEKILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKY
HHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
LQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV
HHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHH
KRISKWGIVTQATSPSFSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVN
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCC
PAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRENPVAVPA
HHHCCHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MYEWHWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDE
CCEEEEEEEECCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
FIYHEALVHPLLLSINNPEKILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKY
HHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
LQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV
HHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHH
KRISKWGIVTQATSPSFSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVN
HHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCC
PAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTRENPVAVPA
HHHCCHHHHHHHHHHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479