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The map label for this gene is purA [H]

Identifier: 15920483

GI number: 15920483

Start: 310370

End: 311377

Strand: Direct

Name: purA [H]

Synonym: ST0291

Alternate gene names: 15920483

Gene position: 310370-311377 (Clockwise)

Preceding gene: 15920482

Following gene: 15920484

Centisome position: 11.52

GC content: 33.83

Gene sequence:

>1008_bases
ATGCTAAACATCCTTGTAGGAGGATTTTTTGGCGATGAAGGTAAGGGAAAAGTTGCAGCATACCTTTCATTAAAAGATTC
CCCATCTCTTTCTGTAAGAACTGGATCAATTAACGCTGGGCATACTGTAACATATATGGGAAAACAATGGAAATTAAGAA
TTATTCCCTCAGCTTTTGTAAATAGAACCACATATTTAGCATTAGCTCCAGGGGCTTTAACTTCAATTGAAGTATTAATA
AATGAGGCAAGAGAGACTAACTCATTAGATCGATTGTATATAGATCCACATGTTGGTATTATAACTGAGAAGGAAATCGA
GGAAGAGAGAAATGATGAATACTTAATGAAGAGAGTAGGAAGTACAGGACAAGGAGTAGGATATGCTGAAGCAAAAAGAA
TTCTAAGAAAATTAAAACTAGCTAAAGATTATGATATATTATCTAAATTTTTGATCAATGTTCCAAATTTAGTTATAGAG
AAATTAGAAAAGAATGAGACAGTTTTAATTGAAGGCACACAAGGTTACTACTTAAGCTTATACCACGGAGAATATCCTTA
TGTAACAAGTAGAAATACTTCTTCATCAGGTGTTTTAAGCGAAGTAGGTATAGGACCAAAATACGTAGATCATGTAATAG
TCGTATTCAAATCCTATGTGACAAGAGTAGGTGAGGGACCTTTAGAAGGAGAATTAGATTGGGAAGAAGCTCAAAGGCTA
GGGATTGCTGAAATAGCCACAGTTACTGGAAGAAAAAGAAGAAGTGCTCCATTTAATATTAAGCTTGCAAAAGAAGCAAT
AAGAGCTAACTCAGCTACTCAAATTGCTATAACTAAATTAGATGCATTATTTAAAGATGCTAAAGGAGTAAAAGAATATT
CAAAACTTCCTCAAGAAGCTAAGAAATGGATAGAAGATATAGAGGAACAGTTAAAAGTACCAATCACACTAATTGGAACT
GGAGAAGACACACTGGATATGATAGACTTGAGAAAAGAAAAGCTATGA

Upstream 100 bases:

>100_bases
TTACACATAAGACTACCTAATTTCTCAGGTATCTATAATTATAAATCAGTATTAGCTAAGTTTATATATTTTTAAGAGAG
ATTTATCAAAACGGTGTTTT

Downstream 100 bases:

>100_bases
GGTGAAATAGATGTTCTTTGACACTATAATAATTGGTGCCGGACCTGCTGGATTATTTGCGGCTTATGAATTAGCAAATC
TAAATAAGAATGATGTGAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLNILVGGFFGDEGKGKVAAYLSLKDSPSLSVRTGSINAGHTVTYMGKQWKLRIIPSAFVNRTTYLALAPGALTSIEVLI
NEARETNSLDRLYIDPHVGIITEKEIEEERNDEYLMKRVGSTGQGVGYAEAKRILRKLKLAKDYDILSKFLINVPNLVIE
KLEKNETVLIEGTQGYYLSLYHGEYPYVTSRNTSSSGVLSEVGIGPKYVDHVIVVFKSYVTRVGEGPLEGELDWEEAQRL
GIAEIATVTGRKRRSAPFNIKLAKEAIRANSATQIAITKLDALFKDAKGVKEYSKLPQEAKKWIEDIEEQLKVPITLIGT
GEDTLDMIDLRKEKL

Sequences:

>Translated_335_residues
MLNILVGGFFGDEGKGKVAAYLSLKDSPSLSVRTGSINAGHTVTYMGKQWKLRIIPSAFVNRTTYLALAPGALTSIEVLI
NEARETNSLDRLYIDPHVGIITEKEIEEERNDEYLMKRVGSTGQGVGYAEAKRILRKLKLAKDYDILSKFLINVPNLVIE
KLEKNETVLIEGTQGYYLSLYHGEYPYVTSRNTSSSGVLSEVGIGPKYVDHVIVVFKSYVTRVGEGPLEGELDWEEAQRL
GIAEIATVTGRKRRSAPFNIKLAKEAIRANSATQIAITKLDALFKDAKGVKEYSKLPQEAKKWIEDIEEQLKVPITLIGT
GEDTLDMIDLRKEKL
>Mature_335_residues
MLNILVGGFFGDEGKGKVAAYLSLKDSPSLSVRTGSINAGHTVTYMGKQWKLRIIPSAFVNRTTYLALAPGALTSIEVLI
NEARETNSLDRLYIDPHVGIITEKEIEEERNDEYLMKRVGSTGQGVGYAEAKRILRKLKLAKDYDILSKFLINVPNLVIE
KLEKNETVLIEGTQGYYLSLYHGEYPYVTSRNTSSSGVLSEVGIGPKYVDHVIVVFKSYVTRVGEGPLEGELDWEEAQRL
GIAEIATVTGRKRRSAPFNIKLAKEAIRANSATQIAITKLDALFKDAKGVKEYSKLPQEAKKWIEDIEEQLKVPITLIGT
GEDTLDMIDLRKEKL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=27.1662763466042, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=25.7009345794392, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI40316944, Length=395, Percent_Identity=24.8101265822785, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=29.4811320754717, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=25.9433962264151, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=25.9433962264151, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324109, Length=422, Percent_Identity=26.5402843601896, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=26.9503546099291, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 37282; Mature: 37282

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNILVGGFFGDEGKGKVAAYLSLKDSPSLSVRTGSINAGHTVTYMGKQWKLRIIPSAFV
CCEEEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCEEEEECCEEEEEECCHHHH
NRTTYLALAPGALTSIEVLINEARETNSLDRLYIDPHVGIITEKEIEEERNDEYLMKRVG
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHCCHHHHHHHCC
STGQGVGYAEAKRILRKLKLAKDYDILSKFLINVPNLVIEKLEKNETVLIEGTQGYYLSL
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEE
YHGEYPYVTSRNTSSSGVLSEVGIGPKYVDHVIVVFKSYVTRVGEGPLEGELDWEEAQRL
EECCCCEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
GIAEIATVTGRKRRSAPFNIKLAKEAIRANSATQIAITKLDALFKDAKGVKEYSKLPQEA
CHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHCHHHH
KKWIEDIEEQLKVPITLIGTGEDTLDMIDLRKEKL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLNILVGGFFGDEGKGKVAAYLSLKDSPSLSVRTGSINAGHTVTYMGKQWKLRIIPSAFV
CCEEEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCEEEEECCEEEEEECCHHHH
NRTTYLALAPGALTSIEVLINEARETNSLDRLYIDPHVGIITEKEIEEERNDEYLMKRVG
CCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHHCCHHHHHHHCC
STGQGVGYAEAKRILRKLKLAKDYDILSKFLINVPNLVIEKLEKNETVLIEGTQGYYLSL
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEE
YHGEYPYVTSRNTSSSGVLSEVGIGPKYVDHVIVVFKSYVTRVGEGPLEGELDWEEAQRL
EECCCCEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
GIAEIATVTGRKRRSAPFNIKLAKEAIRANSATQIAITKLDALFKDAKGVKEYSKLPQEA
CHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCEEEHHHHHHHHHHHCCHHHHHHCHHHH
KKWIEDIEEQLKVPITLIGTGEDTLDMIDLRKEKL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA