Click here to switch to the map view.

The map label for this gene is purP

Identifier: 15920481

GI number: 15920481

Start: 308207

End: 309208

Strand: Direct

Name: purP

Synonym: ST0289

Alternate gene names: 15920481

Gene position: 308207-309208 (Clockwise)

Preceding gene: 15920477

Following gene: 15920482

Centisome position: 11.44

GC content: 31.54

Gene sequence:

>1002_bases
ATGTACATACTTACGATAGGAAGTCATTCATCATTACAAATATTACATGGAGCAAAAAAAGAAGGGTTTAAAACAGCTTT
AGTAACGCCAGAAAAGAGAGTAAAATTCTACAAACAATTTACTTTCATAGATGAAGTTTATGGATACAAAAATGAAGATG
AGGCAGTAGATTATATTAATGACTTTGCAAATAACGGTATACTTATCCCTCACGGGAGTTTAGTAGAATATATTGGACCC
GAAAGAGTAAATAAGATAAAAACAAAAATTTTTGGAAATAGGAATCTGTTCGAATGGGAAGCCAATCAGAAAAAGAAAAT
GAGTTTATTAAAGAGTGCAAAAATAAAAATCCCCGAACAATTTGAAAATCCAGAAGATATAGACAGATTAGTTATAATTA
AGTTACCAGGTGCTAAGGGAGGAAAAGGTTATTTTATAGCTAGAAATAAAAGTGAAGCAAAAGAAGGTTTAAATAAATTA
TTAGAACAAAAAATGATAAGAAGCATAGATGAAGTAATAATCCAAGAATATGTAATAGGTGTTCCTATGTATTTTCAATT
TTTCAATAGTATCATATTAAATAGACTAGAAATTATGGGTATCGATATTAGGTATGAAACGAATATTGATGGACTAAGAA
GACTTCCAGCAGATATTAAGATAGACCCTACTTTAGTTGTAGTAGGTAACATACCTGCAGTGGCTAGAGAAAGTCTTTTA
CCCCTCGTATACGAATACGGTGAAAATTTTGTTAATACGGTAAAAGAATTGGTACCTCCAGGAATGATTGGACCTTTCTG
TTTAGAATCAGTAGTAACTGATCAAGGAGACATAGTGGTATTTGAATTCTCTGGAAGAATAGTAGCTGGAACTAATCTTT
ATGTTAATGGAAGTCCATATAGTTGGCTTTATTGGGATGAACCCATGAGCGTTGGCAGAAGAATCAGTAGAGAGATAAAA
TTAGCAATAAACTCTAATAAGTTAGAACAGGTGTTAACATAA

Upstream 100 bases:

>100_bases
ATTCTCTCATCTCTTTGCTTCCGTATCTCCAGTCTAATACACAAACTGAATTTTCCACGGGAGAAATTATTTATTAAGTA
GTATATGTGATTTTTTATTA

Downstream 100 bases:

>100_bases
TGGTTAGAATAGCTGCTCTTGCAAGTCATTCTGCCCTTGATGTTTTTGATGGAGCTAAAGATGAAAATTTTGAGACTATA
GCTCTTTGTAAAAAAGGTAG

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MYILTIGSHSSLQILHGAKKEGFKTALVTPEKRVKFYKQFTFIDEVYGYKNEDEAVDYINDFANNGILIPHGSLVEYIGP
ERVNKIKTKIFGNRNLFEWEANQKKKMSLLKSAKIKIPEQFENPEDIDRLVIIKLPGAKGGKGYFIARNKSEAKEGLNKL
LEQKMIRSIDEVIIQEYVIGVPMYFQFFNSIILNRLEIMGIDIRYETNIDGLRRLPADIKIDPTLVVVGNIPAVARESLL
PLVYEYGENFVNTVKELVPPGMIGPFCLESVVTDQGDIVVFEFSGRIVAGTNLYVNGSPYSWLYWDEPMSVGRRISREIK
LAINSNKLEQVLT

Sequences:

>Translated_333_residues
MYILTIGSHSSLQILHGAKKEGFKTALVTPEKRVKFYKQFTFIDEVYGYKNEDEAVDYINDFANNGILIPHGSLVEYIGP
ERVNKIKTKIFGNRNLFEWEANQKKKMSLLKSAKIKIPEQFENPEDIDRLVIIKLPGAKGGKGYFIARNKSEAKEGLNKL
LEQKMIRSIDEVIIQEYVIGVPMYFQFFNSIILNRLEIMGIDIRYETNIDGLRRLPADIKIDPTLVVVGNIPAVARESLL
PLVYEYGENFVNTVKELVPPGMIGPFCLESVVTDQGDIVVFEFSGRIVAGTNLYVNGSPYSWLYWDEPMSVGRRISREIK
LAINSNKLEQVLT
>Mature_333_residues
MYILTIGSHSSLQILHGAKKEGFKTALVTPEKRVKFYKQFTFIDEVYGYKNEDEAVDYINDFANNGILIPHGSLVEYIGP
ERVNKIKTKIFGNRNLFEWEANQKKKMSLLKSAKIKIPEQFENPEDIDRLVIIKLPGAKGGKGYFIARNKSEAKEGLNKL
LEQKMIRSIDEVIIQEYVIGVPMYFQFFNSIILNRLEIMGIDIRYETNIDGLRRLPADIKIDPTLVVVGNIPAVARESLL
PLVYEYGENFVNTVKELVPPGMIGPFCLESVVTDQGDIVVFEFSGRIVAGTNLYVNGSPYSWLYWDEPMSVGRRISREIK
LAINSNKLEQVLT

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_SULTO (Q975Y9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376150.1
- ProteinModelPortal:   Q975Y9
- SMR:   Q975Y9
- GeneID:   1458194
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0289
- NMPDR:   fig|273063.1.peg.318
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- BioCyc:   STOK273063:ST0289-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 37892; Mature: 37892

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 9-9 BINDING 73-73 BINDING 206-206 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYILTIGSHSSLQILHGAKKEGFKTALVTPEKRVKFYKQFTFIDEVYGYKNEDEAVDYIN
CEEEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DFANNGILIPHGSLVEYIGPERVNKIKTKIFGNRNLFEWEANQKKKMSLLKSAKIKIPEQ
HHCCCCEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
FENPEDIDRLVIIKLPGAKGGKGYFIARNKSEAKEGLNKLLEQKMIRSIDEVIIQEYVIG
CCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPMYFQFFNSIILNRLEIMGIDIRYETNIDGLRRLPADIKIDPTLVVVGNIPAVARESLL
CHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHCCCCCEECCEEEEEECCCHHHHHHHH
PLVYEYGENFVNTVKELVPPGMIGPFCLESVVTDQGDIVVFEFSGRIVAGTNLYVNGSPY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCEEEECCEEEECCCCC
SWLYWDEPMSVGRRISREIKLAINSNKLEQVLT
EEEEECCHHHHHHHHHHHEEEEECCCHHHHHCC
>Mature Secondary Structure
MYILTIGSHSSLQILHGAKKEGFKTALVTPEKRVKFYKQFTFIDEVYGYKNEDEAVDYIN
CEEEEECCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DFANNGILIPHGSLVEYIGPERVNKIKTKIFGNRNLFEWEANQKKKMSLLKSAKIKIPEQ
HHCCCCEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHH
FENPEDIDRLVIIKLPGAKGGKGYFIARNKSEAKEGLNKLLEQKMIRSIDEVIIQEYVIG
CCCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPMYFQFFNSIILNRLEIMGIDIRYETNIDGLRRLPADIKIDPTLVVVGNIPAVARESLL
CHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHCCCCCEECCEEEEEECCCHHHHHHHH
PLVYEYGENFVNTVKELVPPGMIGPFCLESVVTDQGDIVVFEFSGRIVAGTNLYVNGSPY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCEEEECCEEEECCCCC
SWLYWDEPMSVGRRISREIKLAINSNKLEQVLT
EEEEECCHHHHHHHHHHHEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479