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The map label for this gene is fen

Identifier: 15920393

GI number: 15920393

Start: 233947

End: 234861

Strand: Direct

Name: fen

Synonym: ST0210

Alternate gene names: 15920393

Gene position: 233947-234861 (Clockwise)

Preceding gene: 15920392

Following gene: 15920394

Centisome position: 8.68

GC content: 32.9

Gene sequence:

>915_bases
ATGGATAGTCAAGGAAGAGTAACTAGTCATCTTAATGGATTATTTTATAGGACTATAAGTATTCTGGAGGAGGGAATTAT
ACCCATTTATGTTTTTGATGGAAAACCACCAGAACAAAAAGCTCAAGAATTAGAACGAAGAAAAAAAGTAAAAGAAGAAG
CTGAAAAGAAGTTAGAGCAAGCCAAAACAGAAGGAAGTATAAAGACTAGTGAGTTAAAAAAATATGCTCAAATGAGTATT
AGACTTACTAACGAAATGGCTGAAGAAAGCAAGGAATTACTGAAAGCTATGGGAATTCCAGTAGTTCAAGCACCTTCAGA
AGGTGAAGCAGAGGCAGCATATATAAATATTTTAGGCTTAAGTTGGGCAACTGCAAGCCAGGACTATGACTCCCTTTTAT
TTGGTGCTAAAAGGTTAATAAGAAATCTTACTTTAAGTGGTAAAAGGAAACTTCCGGGAAAAGATGTATATGTAGAGATT
AAACCAGAACTAATAGAACTAGATACTTTATTAAAAAAACTGGGTTTAACTAGAGAACAATTAATAGACATTGGCATTAT
AGTTGGAACAGATTATAACCCAGATGGAATTAAAGGCTATGGCGTTAAAACCGCATATAGAATTATTAAAAAATATGGTA
GTTTAGAAAAAGCAATAGAAAAAGGAGAAATACCAAAAATTAAAGTCAATTTTAACGTCGAAGAGATAAGAAGTCTATTC
CTAAAACCACAAGTAGTAGAGCCTAAGGAAAATCTTGAATTAGTAGATTGTGATAGTAATAAAATATTAGATATCCTAGT
TAAAACGCACGATTTTAACGAGGAAAGAGTAAAAAACGGGATAGAGAGATTAGAAAAAGCAAAGAGAGAAGCAAAAGGAG
CTTCTCGTCAGACTGGACTTGATCAATGGTTTTGA

Upstream 100 bases:

>100_bases
ATCTTTCGCTGAACTTAAAGGAAAGAAAATAAGTATTGATGCATATAATGCCCTTTATCAATTTTTGGCTGCGATAAGAC
AACCAGATGGAACACCATTA

Downstream 100 bases:

>100_bases
TTAACTAAATTTTCATGTACATAGCTTTTAAATTAAATAATTTTATAATGATATTTGTGAACACTCACACTTTCATTCCA
TTATACCTTAATCTGATTGA

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDSQGRVTSHLNGLFYRTISILEEGIIPIYVFDGKPPEQKAQELERRKKVKEEAEKKLEQAKTEGSIKTSELKKYAQMSI
RLTNEMAEESKELLKAMGIPVVQAPSEGEAEAAYINILGLSWATASQDYDSLLFGAKRLIRNLTLSGKRKLPGKDVYVEI
KPELIELDTLLKKLGLTREQLIDIGIIVGTDYNPDGIKGYGVKTAYRIIKKYGSLEKAIEKGEIPKIKVNFNVEEIRSLF
LKPQVVEPKENLELVDCDSNKILDILVKTHDFNEERVKNGIERLEKAKREAKGASRQTGLDQWF

Sequences:

>Translated_304_residues
MDSQGRVTSHLNGLFYRTISILEEGIIPIYVFDGKPPEQKAQELERRKKVKEEAEKKLEQAKTEGSIKTSELKKYAQMSI
RLTNEMAEESKELLKAMGIPVVQAPSEGEAEAAYINILGLSWATASQDYDSLLFGAKRLIRNLTLSGKRKLPGKDVYVEI
KPELIELDTLLKKLGLTREQLIDIGIIVGTDYNPDGIKGYGVKTAYRIIKKYGSLEKAIEKGEIPKIKVNFNVEEIRSLF
LKPQVVEPKENLELVDCDSNKILDILVKTHDFNEERVKNGIERLEKAKREAKGASRQTGLDQWF
>Mature_304_residues
MDSQGRVTSHLNGLFYRTISILEEGIIPIYVFDGKPPEQKAQELERRKKVKEEAEKKLEQAKTEGSIKTSELKKYAQMSI
RLTNEMAEESKELLKAMGIPVVQAPSEGEAEAAYINILGLSWATASQDYDSLLFGAKRLIRNLTLSGKRKLPGKDVYVEI
KPELIELDTLLKKLGLTREQLIDIGIIVGTDYNPDGIKGYGVKTAYRIIKKYGSLEKAIEKGEIPKIKVNFNVEEIRSLF
LKPQVVEPKENLELVDCDSNKILDILVKTHDFNEERVKNGIERLEKAKREAKGASRQTGLDQWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=307, Percent_Identity=36.1563517915309, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI194018535, Length=201, Percent_Identity=30.3482587064677, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI194018531, Length=201, Percent_Identity=30.3482587064677, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI51988900, Length=185, Percent_Identity=30.8108108108108, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17510005, Length=304, Percent_Identity=34.2105263157895, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17555016, Length=206, Percent_Identity=26.2135922330097, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322736, Length=293, Percent_Identity=39.2491467576792, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320469, Length=191, Percent_Identity=31.413612565445, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324607, Length=222, Percent_Identity=27.4774774774775, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321697, Length=137, Percent_Identity=32.1167883211679, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17647423, Length=304, Percent_Identity=39.8026315789474, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24658219, Length=207, Percent_Identity=28.9855072463768, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI78706854, Length=143, Percent_Identity=30.0699300699301, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI78706850, Length=143, Percent_Identity=30.0699300699301, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI78706852, Length=143, Percent_Identity=30.0699300699301, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_SULTO (Q976H6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376062.1
- ProteinModelPortal:   Q976H6
- SMR:   Q976H6
- GeneID:   1458101
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0210
- NMPDR:   fig|273063.1.peg.230
- HOGENOM:   HBG316651
- ProtClustDB:   PRK03980
- BioCyc:   STOK273063:ST0210-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 34393; Mature: 34393

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQGRVTSHLNGLFYRTISILEEGIIPIYVFDGKPPEQKAQELERRKKVKEEAEKKLEQ
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKTEGSIKTSELKKYAQMSIRLTNEMAEESKELLKAMGIPVVQAPSEGEAEAAYINILGL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEC
SWATASQDYDSLLFGAKRLIRNLTLSGKRKLPGKDVYVEIKPELIELDTLLKKLGLTREQ
CHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
LIDIGIIVGTDYNPDGIKGYGVKTAYRIIKKYGSLEKAIEKGEIPKIKVNFNVEEIRSLF
HHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
LKPQVVEPKENLELVDCDSNKILDILVKTHDFNEERVKNGIERLEKAKREAKGASRQTGL
CCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCC
DQWF
CCCC
>Mature Secondary Structure
MDSQGRVTSHLNGLFYRTISILEEGIIPIYVFDGKPPEQKAQELERRKKVKEEAEKKLEQ
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKTEGSIKTSELKKYAQMSIRLTNEMAEESKELLKAMGIPVVQAPSEGEAEAAYINILGL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEEEEC
SWATASQDYDSLLFGAKRLIRNLTLSGKRKLPGKDVYVEIKPELIELDTLLKKLGLTREQ
CHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
LIDIGIIVGTDYNPDGIKGYGVKTAYRIIKKYGSLEKAIEKGEIPKIKVNFNVEEIRSLF
HHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
LKPQVVEPKENLELVDCDSNKILDILVKTHDFNEERVKNGIERLEKAKREAKGASRQTGL
CCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCC
DQWF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479