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The map label for this gene is fadE12 [H]

Identifier: 15920245

GI number: 15920245

Start: 88099

End: 89268

Strand: Direct

Name: fadE12 [H]

Synonym: ST0066

Alternate gene names: 15920245

Gene position: 88099-89268 (Clockwise)

Preceding gene: 15920243

Following gene: 15920248

Centisome position: 3.27

GC content: 34.27

Gene sequence:

>1170_bases
GTGTTACTTTCGCCTAAAGATGAAGAAGAGAAGCTAATCCTCTCAACTGTAGATGAGTTAATGAAGAAATATGATGAGAT
ATATTGGCTAAATAAAGACCAAAGAAGAGAATTTCCAGTGGAGTTTTTTAATGATTTCATGAGTTTAGGTTTGGGATCAA
TACTAATACCTATAGAATATGGAGGGGCTGGTAAAGGTATTAGGTTAGCTTGCTTAATCCTTTACCTAATTAATGTAAGA
GGAGGTAACTCATATTTCGTTCATGGACAATATTACACTACAGCATTACTTGTAAGACATTCAAATAAAAAGTTAAAGGA
TAAATATTTTAAAGATATAGGTAAAGGCGAGAAGGTATTATCATTAGCATTAACCGAGCCAGATGTAGGATCTGATACTA
CTAGAATGAAGACTATTGCAGAAAAAGTCGGAGATAAGTATATTGTAAATGGCCATAAGATTTTCATTTCTAGGTTGAAA
TACACTGATTTTATAATATTAGTGGCTAGGACAACGCCTTATGAGAGGGTTGAAAAGAAGACTGATGGGATTACTCTATT
TCTAGTTGATTTGAGAGAAGGAAGAAAGGGAATTGAAATGAGAGAGATTAGAACTATGTCAAATACCGATGCATATGAAG
TTTTCATTGATAATTTAGAAATACCTGAGGAAAATGTTATAGGAGAAGTAGGTAAGGGATTTTACCATTTACTAGATTTG
TTAAATACTGAAAGATTTATGATAGCCGCTGAATTAATAGGTAATGCTGAATGGTTTATAAATAAGGCTGTGGATTATGC
TAAAAATAGGGTAGTTTTCGGTAAGCCAATAGGCAGTTTTAAAGGCGTTCAATTTCCAATAGCCAAGGTATATGCTGAGT
TAAAAGCACTTGTAAGTTATTTCAATGAAGGATTAAGTTTAGCAGAGGAGGGAAAGGACACTAAATTAATTGGAAACTAT
GCCAATATTTCAAAATATCTAGCCGCAGAAATAGCCTGGGAAGCAGGGAATGTGACAATGGACATTTATGGAGGTTACGG
TTATGCTGTGGAAACAGGCATAGAGAGGAAGTTTAGAGAGACTAGGCTATATAAGGTAGCCCCAGTATCTCAAAATTTAA
TCTTAGCCTATATTGCTCACCATATGTTAGGACTTCCGAGATCATACTAA

Upstream 100 bases:

>100_bases
ATGTGTTTTTAGTTAATATTAAGGTTATATTTTCTTCGAAATTACTAGAAAAACTGTTTTTAGGAAAACTTTATAATAAA
ACTTTATTAATTATATTATT

Downstream 100 bases:

>100_bases
CGGGAGTAGGATAGCAAGTTGAAACAAGCTTAAAAATTACTTCCAATTTAAGATTTAACCTACTTAGGAAAAATAACTTT
TAATTAGAGGTCTTACGAAA

Product: acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLLSPKDEEEKLILSTVDELMKKYDEIYWLNKDQRREFPVEFFNDFMSLGLGSILIPIEYGGAGKGIRLACLILYLINVR
GGNSYFVHGQYYTTALLVRHSNKKLKDKYFKDIGKGEKVLSLALTEPDVGSDTTRMKTIAEKVGDKYIVNGHKIFISRLK
YTDFIILVARTTPYERVEKKTDGITLFLVDLREGRKGIEMREIRTMSNTDAYEVFIDNLEIPEENVIGEVGKGFYHLLDL
LNTERFMIAAELIGNAEWFINKAVDYAKNRVVFGKPIGSFKGVQFPIAKVYAELKALVSYFNEGLSLAEEGKDTKLIGNY
ANISKYLAAEIAWEAGNVTMDIYGGYGYAVETGIERKFRETRLYKVAPVSQNLILAYIAHHMLGLPRSY

Sequences:

>Translated_389_residues
MLLSPKDEEEKLILSTVDELMKKYDEIYWLNKDQRREFPVEFFNDFMSLGLGSILIPIEYGGAGKGIRLACLILYLINVR
GGNSYFVHGQYYTTALLVRHSNKKLKDKYFKDIGKGEKVLSLALTEPDVGSDTTRMKTIAEKVGDKYIVNGHKIFISRLK
YTDFIILVARTTPYERVEKKTDGITLFLVDLREGRKGIEMREIRTMSNTDAYEVFIDNLEIPEENVIGEVGKGFYHLLDL
LNTERFMIAAELIGNAEWFINKAVDYAKNRVVFGKPIGSFKGVQFPIAKVYAELKALVSYFNEGLSLAEEGKDTKLIGNY
ANISKYLAAEIAWEAGNVTMDIYGGYGYAVETGIERKFRETRLYKVAPVSQNLILAYIAHHMLGLPRSY
>Mature_389_residues
MLLSPKDEEEKLILSTVDELMKKYDEIYWLNKDQRREFPVEFFNDFMSLGLGSILIPIEYGGAGKGIRLACLILYLINVR
GGNSYFVHGQYYTTALLVRHSNKKLKDKYFKDIGKGEKVLSLALTEPDVGSDTTRMKTIAEKVGDKYIVNGHKIFISRLK
YTDFIILVARTTPYERVEKKTDGITLFLVDLREGRKGIEMREIRTMSNTDAYEVFIDNLEIPEENVIGEVGKGFYHLLDL
LNTERFMIAAELIGNAEWFINKAVDYAKNRVVFGKPIGSFKGVQFPIAKVYAELKALVSYFNEGLSLAEEGKDTKLIGNY
ANISKYLAAEIAWEAGNVTMDIYGGYGYAVETGIERKFRETRLYKVAPVSQNLILAYIAHHMLGLPRSY

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958414, Length=358, Percent_Identity=29.8882681564246, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226958412, Length=372, Percent_Identity=28.494623655914, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4501859, Length=380, Percent_Identity=28.6842105263158, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4557235, Length=393, Percent_Identity=28.498727735369, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI76496475, Length=393, Percent_Identity=28.498727735369, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4501857, Length=273, Percent_Identity=29.3040293040293, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI187960098, Length=341, Percent_Identity=27.8592375366569, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4557231, Length=341, Percent_Identity=27.8592375366569, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI7656849, Length=361, Percent_Identity=27.1468144044321, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI21361497, Length=355, Percent_Identity=27.6056338028169, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4557233, Length=392, Percent_Identity=26.7857142857143, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI7669494, Length=204, Percent_Identity=28.4313725490196, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4503943, Length=204, Percent_Identity=28.4313725490196, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786223, Length=382, Percent_Identity=26.7015706806283, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87081958, Length=375, Percent_Identity=26.9333333333333, Blast_Score=137, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71985184, Length=389, Percent_Identity=28.2776349614396, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17538396, Length=344, Percent_Identity=29.6511627906977, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17534899, Length=343, Percent_Identity=28.8629737609329, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17569725, Length=361, Percent_Identity=28.2548476454294, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17508101, Length=386, Percent_Identity=27.4611398963731, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551932, Length=347, Percent_Identity=25.6484149855908, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17570075, Length=361, Percent_Identity=27.1468144044321, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71985192, Length=308, Percent_Identity=27.9220779220779, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17506239, Length=340, Percent_Identity=24.4117647058824, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71990804, Length=273, Percent_Identity=30.03663003663, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17505929, Length=325, Percent_Identity=24.9230769230769, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17533517, Length=358, Percent_Identity=24.5810055865922, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982178, Length=269, Percent_Identity=29.368029739777, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32563615, Length=252, Percent_Identity=25, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17534353, Length=258, Percent_Identity=27.1317829457364, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531909, Length=252, Percent_Identity=25.7936507936508, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24666513, Length=377, Percent_Identity=27.8514588859416, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281363737, Length=390, Percent_Identity=28.4615384615385, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355753, Length=329, Percent_Identity=28.8753799392097, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24660351, Length=340, Percent_Identity=26.4705882352941, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356377, Length=344, Percent_Identity=27.906976744186, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646207, Length=386, Percent_Identity=26.9430051813472, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920834, Length=199, Percent_Identity=28.643216080402, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 44258; Mature: 44258

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSPKDEEEKLILSTVDELMKKYDEIYWLNKDQRREFPVEFFNDFMSLGLGSILIPIEY
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEE
GGAGKGIRLACLILYLINVRGGNSYFVHGQYYTTALLVRHSNKKLKDKYFKDIGKGEKVL
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHCCCCCEEE
SLALTEPDVGSDTTRMKTIAEKVGDKYIVNGHKIFISRLKYTDFIILVARTTPYERVEKK
EEEECCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEHCCCCEEEEEEEECCCHHHHHHC
TDGITLFLVDLREGRKGIEMREIRTMSNTDAYEVFIDNLEIPEENVIGEVGKGFYHLLDL
CCCEEEEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LNTERFMIAAELIGNAEWFINKAVDYAKNRVVFGKPIGSFKGVQFPIAKVYAELKALVSY
HCCCHHEEHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
FNEGLSLAEEGKDTKLIGNYANISKYLAAEIAWEAGNVTMDIYGGYGYAVETGIERKFRE
HHHCCCHHHCCCCCEEECCHHHHHHHHHHHHEEECCCEEEEEECCCCEEEHHHHHHHHHH
TRLYKVAPVSQNLILAYIAHHMLGLPRSY
HHCEEECCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLSPKDEEEKLILSTVDELMKKYDEIYWLNKDQRREFPVEFFNDFMSLGLGSILIPIEY
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEE
GGAGKGIRLACLILYLINVRGGNSYFVHGQYYTTALLVRHSNKKLKDKYFKDIGKGEKVL
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHCCCCCEEE
SLALTEPDVGSDTTRMKTIAEKVGDKYIVNGHKIFISRLKYTDFIILVARTTPYERVEKK
EEEECCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEHCCCCEEEEEEEECCCHHHHHHC
TDGITLFLVDLREGRKGIEMREIRTMSNTDAYEVFIDNLEIPEENVIGEVGKGFYHLLDL
CCCEEEEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LNTERFMIAAELIGNAEWFINKAVDYAKNRVVFGKPIGSFKGVQFPIAKVYAELKALVSY
HCCCHHEEHHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
FNEGLSLAEEGKDTKLIGNYANISKYLAAEIAWEAGNVTMDIYGGYGYAVETGIERKFRE
HHHCCCHHHCCCCCEEECCHHHHHHHHHHHHEEECCCEEEEEECCCCEEEHHHHHHHHHH
TRLYKVAPVSQNLILAYIAHHMLGLPRSY
HHCEEECCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]