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The map label for this gene is trpB2 [H]

Identifier: 159041559

GI number: 159041559

Start: 1043722

End: 1045029

Strand: Reverse

Name: trpB2 [H]

Synonym: Cmaq_0990

Alternate gene names: 159041559

Gene position: 1045029-1043722 (Counterclockwise)

Preceding gene: 159041564

Following gene: 159041558

Centisome position: 50.3

GC content: 47.86

Gene sequence:

>1308_bases
TTGGGTAAGTCACTAAGCCAGTATAGGGTAGACCTACCTCCAGATGAAATCCCAGATCACTGGTACAATATACTCGCTGA
TCTTCCTGAACCACTCCCACCGCCTCAGGATCCTGATAATGGGAGGAGACTTGAATTGCTTAAGAAAGTCATACCCTCTG
AGCCCCTAGGCTTCGAATTCTCCACGGAAAGATTCATTAAAATACCTGATGAGGTGCGTGAAAGGTATATCCAGGTTGGT
AGACCAACACCATTGATTAGGGCTAGGAGGTTTGAGGAGTACTTGGATGCCCCAGTTAAAATATACCTTAAAATGGAGGG
TTATACTTACACGGGGAGTCATAAGATTAACTCAGCCTTAGCCTGGGTCTACTATGCGCTTAAGGATGGTGCGAGATTAG
TCACCACTGAGACTGGGGCTGGGCAATGGGGTGCTGCGGTTGCCTTGGCTTCAGCCTTATTTAAGGTTAAGGCACACATA
TTCATGGTTAAGGCAAGCTACAACGCCAAGCCGCTTAGGAGGTTCCAAATGCAGATGTATGGAGCCGACGTTCATGCAAG
CCCCAGTGAATTAACGGAATTCGGAAGGAGGCTACTGAAGGAGAATCCCAATCACCCAGGTAGCCTAGGTGTAGCCATAA
CCGAGGCCGCGGAGTACGCGCTTAATAATGGTGGTAAGTACGTTGTCGGCAGTGTTATAAACACTGACATATTATTTAAG
ACTGTTGCTGGACTTGAGGCTAAGAGGCAGCTTGAGTTAATAGGCGAGGACCCTGATGTAATGATAGGGGTAGTGGGTGG
TGGTAGTAATTGGGGTGGGGCCTTTTACCCATTCATGGGTGATGAACTCAGAAGCGGGAGGGTTAGACGGAGGTATATCG
CCGTAGGCGCCTCTGAGGTGCCTAAGGTTACTAAAGGCGTCTACAAGTATGATGACCCAGACACAGGTAGGGTTCTACCA
CAGTTGAAGATGTACACAATAGGCTCAGACTTCATTCCACCACCCATTTACGCAGGGGGGTTGCGTTATCATGCAGTTGC
CCCAACCTTATCATACTTAATGAGCAAGGGTTACGTGGAGGGGAGGGATTATGACCAGGACACCGTGTTTAAGATGGCTC
AAGTATTCGCACAGGTTGAAGGCTACATACCCGCACCAGAGACTGCGCATACTCTACCAGTAATTAAGGAGATTGCTGAT
GAAGCCAAGAGAACTGGTGAAAGAAAAGTCATATTAGTTAGCTTCAGTGGCCATGGCCTGCTTGACTTAGGTAACTTCGC
TGATGTATTAGGGTTTGGAAAGGCATAA

Upstream 100 bases:

>100_bases
AAGCCTCTTTAAAAATCCTCACCCACTGGTCATTCCAGGCTGTGAATCCTGGGTTTACACTTAAAAACCAAGCCACCTAA
ACCCCATAGGTGGTCACTAT

Downstream 100 bases:

>100_bases
CAATGAAGGTAAACCTTAGTAATAAACCGTTCTTAAACTAATTTAATGCTTCACTTTGTAATATTCATTAAGTTTGTGGC
TAATTTAGTGAAGTCTAAGG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGKSLSQYRVDLPPDEIPDHWYNILADLPEPLPPPQDPDNGRRLELLKKVIPSEPLGFEFSTERFIKIPDEVRERYIQVG
RPTPLIRARRFEEYLDAPVKIYLKMEGYTYTGSHKINSALAWVYYALKDGARLVTTETGAGQWGAAVALASALFKVKAHI
FMVKASYNAKPLRRFQMQMYGADVHASPSELTEFGRRLLKENPNHPGSLGVAITEAAEYALNNGGKYVVGSVINTDILFK
TVAGLEAKRQLELIGEDPDVMIGVVGGGSNWGGAFYPFMGDELRSGRVRRRYIAVGASEVPKVTKGVYKYDDPDTGRVLP
QLKMYTIGSDFIPPPIYAGGLRYHAVAPTLSYLMSKGYVEGRDYDQDTVFKMAQVFAQVEGYIPAPETAHTLPVIKEIAD
EAKRTGERKVILVSFSGHGLLDLGNFADVLGFGKA

Sequences:

>Translated_435_residues
MGKSLSQYRVDLPPDEIPDHWYNILADLPEPLPPPQDPDNGRRLELLKKVIPSEPLGFEFSTERFIKIPDEVRERYIQVG
RPTPLIRARRFEEYLDAPVKIYLKMEGYTYTGSHKINSALAWVYYALKDGARLVTTETGAGQWGAAVALASALFKVKAHI
FMVKASYNAKPLRRFQMQMYGADVHASPSELTEFGRRLLKENPNHPGSLGVAITEAAEYALNNGGKYVVGSVINTDILFK
TVAGLEAKRQLELIGEDPDVMIGVVGGGSNWGGAFYPFMGDELRSGRVRRRYIAVGASEVPKVTKGVYKYDDPDTGRVLP
QLKMYTIGSDFIPPPIYAGGLRYHAVAPTLSYLMSKGYVEGRDYDQDTVFKMAQVFAQVEGYIPAPETAHTLPVIKEIAD
EAKRTGERKVILVSFSGHGLLDLGNFADVLGFGKA
>Mature_434_residues
GKSLSQYRVDLPPDEIPDHWYNILADLPEPLPPPQDPDNGRRLELLKKVIPSEPLGFEFSTERFIKIPDEVRERYIQVGR
PTPLIRARRFEEYLDAPVKIYLKMEGYTYTGSHKINSALAWVYYALKDGARLVTTETGAGQWGAAVALASALFKVKAHIF
MVKASYNAKPLRRFQMQMYGADVHASPSELTEFGRRLLKENPNHPGSLGVAITEAAEYALNNGGKYVVGSVINTDILFKT
VAGLEAKRQLELIGEDPDVMIGVVGGGSNWGGAFYPFMGDELRSGRVRRRYIAVGASEVPKVTKGVYKYDDPDTGRVLPQ
LKMYTIGSDFIPPPIYAGGLRYHAVAPTLSYLMSKGYVEGRDYDQDTVFKMAQVFAQVEGYIPAPETAHTLPVIKEIADE
AKRTGERKVILVSFSGHGLLDLGNFADVLGFGKA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=374, Percent_Identity=28.3422459893048, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=31.25, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 48183; Mature: 48052

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLSQYRVDLPPDEIPDHWYNILADLPEPLPPPQDPDNGRRLELLKKVIPSEPLGFEF
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
STERFIKIPDEVRERYIQVGRPTPLIRARRFEEYLDAPVKIYLKMEGYTYTGSHKINSAL
CCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEECCCHHHHHH
AWVYYALKDGARLVTTETGAGQWGAAVALASALFKVKAHIFMVKASYNAKPLRRFQMQMY
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHH
GADVHASPSELTEFGRRLLKENPNHPGSLGVAITEAAEYALNNGGKYVVGSVINTDILFK
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEHHHHHHHHHH
TVAGLEAKRQLELIGEDPDVMIGVVGGGSNWGGAFYPFMGDELRSGRVRRRYIAVGASEV
HHHCCCHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHCCCCEEEEEEECCHHC
PKVTKGVYKYDDPDTGRVLPQLKMYTIGSDFIPPPIYAGGLRYHAVAPTLSYLMSKGYVE
CHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCEEEEHHHHHHHHHHHCCCCC
GRDYDQDTVFKMAQVFAQVEGYIPAPETAHTLPVIKEIADEAKRTGERKVILVSFSGHGL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LDLGNFADVLGFGKA
EECCHHHHHHCCCCC
>Mature Secondary Structure 
GKSLSQYRVDLPPDEIPDHWYNILADLPEPLPPPQDPDNGRRLELLKKVIPSEPLGFEF
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
STERFIKIPDEVRERYIQVGRPTPLIRARRFEEYLDAPVKIYLKMEGYTYTGSHKINSAL
CCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEECCCHHHHHH
AWVYYALKDGARLVTTETGAGQWGAAVALASALFKVKAHIFMVKASYNAKPLRRFQMQMY
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHH
GADVHASPSELTEFGRRLLKENPNHPGSLGVAITEAAEYALNNGGKYVVGSVINTDILFK
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEHHHHHHHHHH
TVAGLEAKRQLELIGEDPDVMIGVVGGGSNWGGAFYPFMGDELRSGRVRRRYIAVGASEV
HHHCCCHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHCCCCEEEEEEECCHHC
PKVTKGVYKYDDPDTGRVLPQLKMYTIGSDFIPPPIYAGGLRYHAVAPTLSYLMSKGYVE
CHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCEEEEHHHHHHHHHHHCCCCC
GRDYDQDTVFKMAQVFAQVEGYIPAPETAHTLPVIKEIADEAKRTGERKVILVSFSGHGL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
LDLGNFADVLGFGKA
EECCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]