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The map label for this gene is rfcS2 [H]

Identifier: 159041324

GI number: 159041324

Start: 781191

End: 782147

Strand: Reverse

Name: rfcS2 [H]

Synonym: Cmaq_0750

Alternate gene names: 159041324

Gene position: 782147-781191 (Counterclockwise)

Preceding gene: 159041327

Following gene: 159041323

Centisome position: 37.65

GC content: 43.36

Gene sequence:

>957_bases
GTGGAGCGTTTTAGGCCTGTTAGGCTTGTGGATTTGGTTGATCAGGAGGGTGTTAAGGTTGGTTTGATGGAGTTTGTTAG
GCGTGGTGACTTGCCTCACCTACTCTTCTATGGTCCTCCAGGTGTTGGTAAGACTACTGCTGCCTTGGCTTTGGCTAGGG
AGCTTTACGGTGATTCATGGCGTAGTAGTGTTCTTGAGCTTAATGCCAGTGATGAGAGGGGTATTGATGTTATTAGGGAG
AAGGTTAAGGAATTCGCTAGAACAATACCCACCGGCCCAGTACCGTTTAAACTAGTAATCCTTGATGAGGCTGATAACAT
GACTAGTGATGCCCAGCAAGCCTTAAGGAGGATTATGGAGATGTACGCATCAACAACAAGATTCATACTACTAGCCAACT
ACATAAGCGGAATAATAGAACCAATACAATCCAGGTGCGCAATATTCAGATTCAACCCACTACCAAAGGAGGCAGTAATA
GAGAGGCTTAGGCAAATAGCCAAGGAAACTGGAGTAGAGGTAACTGAGGATGGGTTAGAGGCAATATGGGAGGTGAGCCA
AGGAGACATGAGAAAAGCAATAAACACACTACAAACAACAACCACAACAAACAAAAAAGTGGATGAAAACGCCGTTTATC
AACTCTTCGGCGGCATTAATCCTCAGGAGGTTAGGGATTTAATATATGAGGCGTTGAATGGTGACTTTGGAAAGGCTATA
ATGGGTCTTAAATCAATGATAAGGGATAGAGGTGTTGACCCAATTTACATAATTAGGCTACTGCATAGGGAGGTCACTTC
AACAGTCTCTAAGATTAATGCACCCGAGTACCTGAAACCGAGGATAATATACAGTATTGCTATGCGTCATCATGCAATAC
TAAGGGGGAGTGATGACTTAACGCAGGTTATTGGTTTACTCGCAGAAATAAGGTTAATGCTTAAGTCATCAGGCTAA

Upstream 100 bases:

>100_bases
ATTGAAGGTAAGTTACTTGTCCTTAAGATTAATAAGGTGCTTGGAATGGGAAGGTTTATAATTCAATAAATCCCTAGTAA
ATGTGCCTGAGGAGCCTTGG

Downstream 100 bases:

>100_bases
CTGGGTAATGCACCATTAGTGGAAATTAGCTTAATCATTATTCATAATATGAATACAGCATTATGATTGAATGATAATGA
TTATTGTGAAAAACTGAGTA

Product: ATPase central domain-containing protein

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit 2; Clamp loader small subunit 2 [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MERFRPVRLVDLVDQEGVKVGLMEFVRRGDLPHLLFYGPPGVGKTTAALALARELYGDSWRSSVLELNASDERGIDVIRE
KVKEFARTIPTGPVPFKLVILDEADNMTSDAQQALRRIMEMYASTTRFILLANYISGIIEPIQSRCAIFRFNPLPKEAVI
ERLRQIAKETGVEVTEDGLEAIWEVSQGDMRKAINTLQTTTTTNKKVDENAVYQLFGGINPQEVRDLIYEALNGDFGKAI
MGLKSMIRDRGVDPIYIIRLLHREVTSTVSKINAPEYLKPRIIYSIAMRHHAILRGSDDLTQVIGLLAEIRLMLKSSG

Sequences:

>Translated_318_residues
MERFRPVRLVDLVDQEGVKVGLMEFVRRGDLPHLLFYGPPGVGKTTAALALARELYGDSWRSSVLELNASDERGIDVIRE
KVKEFARTIPTGPVPFKLVILDEADNMTSDAQQALRRIMEMYASTTRFILLANYISGIIEPIQSRCAIFRFNPLPKEAVI
ERLRQIAKETGVEVTEDGLEAIWEVSQGDMRKAINTLQTTTTTNKKVDENAVYQLFGGINPQEVRDLIYEALNGDFGKAI
MGLKSMIRDRGVDPIYIIRLLHREVTSTVSKINAPEYLKPRIIYSIAMRHHAILRGSDDLTQVIGLLAEIRLMLKSSG
>Mature_318_residues
MERFRPVRLVDLVDQEGVKVGLMEFVRRGDLPHLLFYGPPGVGKTTAALALARELYGDSWRSSVLELNASDERGIDVIRE
KVKEFARTIPTGPVPFKLVILDEADNMTSDAQQALRRIMEMYASTTRFILLANYISGIIEPIQSRCAIFRFNPLPKEAVI
ERLRQIAKETGVEVTEDGLEAIWEVSQGDMRKAINTLQTTTTTNKKVDENAVYQLFGGINPQEVRDLIYEALNGDFGKAI
MGLKSMIRDRGVDPIYIIRLLHREVTSTVSKINAPEYLKPRIIYSIAMRHHAILRGSDDLTQVIGLLAEIRLMLKSSG

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506491, Length=324, Percent_Identity=41.9753086419753, Blast_Score=227, Evalue=9e-60,
Organism=Homo sapiens, GI31881687, Length=324, Percent_Identity=41.9753086419753, Blast_Score=227, Evalue=9e-60,
Organism=Homo sapiens, GI194306571, Length=313, Percent_Identity=38.9776357827476, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI194306567, Length=313, Percent_Identity=38.9776357827476, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI6677723, Length=313, Percent_Identity=38.9776357827476, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI31563534, Length=319, Percent_Identity=41.3793103448276, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI28882049, Length=316, Percent_Identity=35.7594936708861, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI194306569, Length=249, Percent_Identity=38.1526104417671, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI108773789, Length=224, Percent_Identity=35.2678571428571, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4506489, Length=224, Percent_Identity=35.2678571428571, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71986063, Length=313, Percent_Identity=41.5335463258786, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17541988, Length=311, Percent_Identity=41.1575562700965, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17554730, Length=216, Percent_Identity=45.8333333333333, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17541990, Length=320, Percent_Identity=32.5, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324478, Length=312, Percent_Identity=40.3846153846154, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324039, Length=263, Percent_Identity=41.8250950570342, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6322528, Length=328, Percent_Identity=36.280487804878, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6319562, Length=242, Percent_Identity=30.5785123966942, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17647857, Length=313, Percent_Identity=43.1309904153355, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921076, Length=310, Percent_Identity=39.6774193548387, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI18859927, Length=327, Percent_Identity=37.0030581039755, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI19921136, Length=300, Percent_Identity=27.6666666666667, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 35786; Mature: 35786

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFRPVRLVDLVDQEGVKVGLMEFVRRGDLPHLLFYGPPGVGKTTAALALARELYGDSW
CCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHH
RSSVLELNASDERGIDVIREKVKEFARTIPTGPVPFKLVILDEADNMTSDAQQALRRIME
HHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
MYASTTRFILLANYISGIIEPIQSRCAIFRFNPLPKEAVIERLRQIAKETGVEVTEDGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
AIWEVSQGDMRKAINTLQTTTTTNKKVDENAVYQLFGGINPQEVRDLIYEALNGDFGKAI
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
MGLKSMIRDRGVDPIYIIRLLHREVTSTVSKINAPEYLKPRIIYSIAMRHHAILRGSDDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
TQVIGLLAEIRLMLKSSG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERFRPVRLVDLVDQEGVKVGLMEFVRRGDLPHLLFYGPPGVGKTTAALALARELYGDSW
CCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHH
RSSVLELNASDERGIDVIREKVKEFARTIPTGPVPFKLVILDEADNMTSDAQQALRRIME
HHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
MYASTTRFILLANYISGIIEPIQSRCAIFRFNPLPKEAVIERLRQIAKETGVEVTEDGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
AIWEVSQGDMRKAINTLQTTTTTNKKVDENAVYQLFGGINPQEVRDLIYEALNGDFGKAI
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
MGLKSMIRDRGVDPIYIIRLLHREVTSTVSKINAPEYLKPRIIYSIAMRHHAILRGSDDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
TQVIGLLAEIRLMLKSSG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA