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The map label for this gene is purT [H]

Identifier: 159041238

GI number: 159041238

Start: 695752

End: 696951

Strand: Reverse

Name: purT [H]

Synonym: Cmaq_0660

Alternate gene names: 159041238

Gene position: 696951-695752 (Counterclockwise)

Preceding gene: 159041239

Following gene: 159041236

Centisome position: 33.55

GC content: 46.08

Gene sequence:

>1200_bases
ATGCTCGGCACACCATTGCTTGAGGGGGGTAGGAGAATAATGATACTTGGTGGTGGTGAATTGGGTAAGGAAATTGCCAT
TGAGGCTCAACGCATGGGTGTTGAAGTGATTGTTGTTGATAGGTATGACATGGCGCCAGCCATGCATGTAGCCCATAGGA
GTTACGTAATTGATATGCTTAATGGCGATGCCTTAAAGGCTATTGTGAGGAGGGAGAAACCGGATGCAGTGGTTACTGAG
ATTGAGGCAATTAACACAGATGCCTTAATTGACCTTGAGAATGAGGGGTTTAGAATAATCCCGAACGCCAATGCCGTTAA
AATATGCATGAATAGGATTGAATTAAGGCTACTGGCCTCGGAACGGCTTAAATTACCCACAACTAGGTATGCCGTAGCCA
ATGACCCTGGGGAGGCTAGGAGAGCTTGTAGTGATGTTGGTTACCCCTGCCTTATTAAGCCTGAAATGAGCTCCAGCGGC
CATGGTCACGAAGTTGTAGTTAACCCCAGTGATGTTGAATCCAAGTTTAATGAAGCCATTAAGCATGCTAGAGGGAGTAG
TAGGAGGGTTATTGTTGAGGAGTACGTTGACGTGGATAAGGAGTTAACGGTATTAACGTACAGGTACCCGAGTGAAGGCA
GTACGGTGACAGCTGTTATACCACCGATTGAGCATCAGAGGCCTAGGGGTGTGTTTCACTATGTTGAGTCATGGCATCCA
TCAAGTGTTAGTGGTAATATAATTAAGACCGCTGAGGATTATGCAGTGAGGATGGTTAATGAATTAGGAGGCTTAGGTAT
ATTTGGGGTGGAGATTATAATAACTAGGGATGGCAGAGTACTCTTCAGTGAGGTTGCCCCAAGACCCCATGATACTGGTT
TAGTGACGCTTGCCAGTACTGATATTAATGAATTCCAGGTTCACGTAAGGAGCGCATTAGGCCTACCCACTCCTCAGGTT
AAGGTTACTACACCAGCTGCTGCACACGTAATACTGGCTGATAATGAAGCCTGGGCCCCTCGTTACATCGGCATTAATGA
GGCTTTAACCATACCTGGAGTTCAGGTTAGGTTATTTGGTAAACCAAACTCATACAGGGGGAGGAGGATGGGTGTTATTT
TGGCAACGGGCTCAACAGTGGAGGAGGCTATTGAAAAAGTTAGGAAGGCCGCCAGTATCGTGAAAGTAGAGAGTACTTAA

Upstream 100 bases:

>100_bases
CTGAAAGGGCTGCTGAGGATACATTGGTACCGAGGGGTTTCGATAAAGGTGGCTTAAGGCTTTGGGTCAGTTTAAGGGAA
AGCCTCAGGAGGGGATGGTG

Downstream 100 bases:

>100_bases
TTTAAGATGAGGCTAGTAAGATTCCTATTATTGATAGGTATTTTCAACCATGCCTGATTTAAATTTAATCATGACTTAAA
TAAATGATGCTTTCAACGTA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLGTPLLEGGRRIMILGGGELGKEIAIEAQRMGVEVIVVDRYDMAPAMHVAHRSYVIDMLNGDALKAIVRREKPDAVVTE
IEAINTDALIDLENEGFRIIPNANAVKICMNRIELRLLASERLKLPTTRYAVANDPGEARRACSDVGYPCLIKPEMSSSG
HGHEVVVNPSDVESKFNEAIKHARGSSRRVIVEEYVDVDKELTVLTYRYPSEGSTVTAVIPPIEHQRPRGVFHYVESWHP
SSVSGNIIKTAEDYAVRMVNELGGLGIFGVEIIITRDGRVLFSEVAPRPHDTGLVTLASTDINEFQVHVRSALGLPTPQV
KVTTPAAAHVILADNEAWAPRYIGINEALTIPGVQVRLFGKPNSYRGRRMGVILATGSTVEEAIEKVRKAASIVKVEST

Sequences:

>Translated_399_residues
MLGTPLLEGGRRIMILGGGELGKEIAIEAQRMGVEVIVVDRYDMAPAMHVAHRSYVIDMLNGDALKAIVRREKPDAVVTE
IEAINTDALIDLENEGFRIIPNANAVKICMNRIELRLLASERLKLPTTRYAVANDPGEARRACSDVGYPCLIKPEMSSSG
HGHEVVVNPSDVESKFNEAIKHARGSSRRVIVEEYVDVDKELTVLTYRYPSEGSTVTAVIPPIEHQRPRGVFHYVESWHP
SSVSGNIIKTAEDYAVRMVNELGGLGIFGVEIIITRDGRVLFSEVAPRPHDTGLVTLASTDINEFQVHVRSALGLPTPQV
KVTTPAAAHVILADNEAWAPRYIGINEALTIPGVQVRLFGKPNSYRGRRMGVILATGSTVEEAIEKVRKAASIVKVEST
>Mature_399_residues
MLGTPLLEGGRRIMILGGGELGKEIAIEAQRMGVEVIVVDRYDMAPAMHVAHRSYVIDMLNGDALKAIVRREKPDAVVTE
IEAINTDALIDLENEGFRIIPNANAVKICMNRIELRLLASERLKLPTTRYAVANDPGEARRACSDVGYPCLIKPEMSSSG
HGHEVVVNPSDVESKFNEAIKHARGSSRRVIVEEYVDVDKELTVLTYRYPSEGSTVTAVIPPIEHQRPRGVFHYVESWHP
SSVSGNIIKTAEDYAVRMVNELGGLGIFGVEIIITRDGRVLFSEVAPRPHDTGLVTLASTDINEFQVHVRSALGLPTPQV
KVTTPAAAHVILADNEAWAPRYIGINEALTIPGVQVRLFGKPNSYRGRRMGVILATGSTVEEAIEKVRKAASIVKVEST

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=399, Percent_Identity=47.3684210526316, Blast_Score=342, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324702, Length=396, Percent_Identity=25.7575757575758, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43664; Mature: 43664

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTPLLEGGRRIMILGGGELGKEIAIEAQRMGVEVIVVDRYDMAPAMHVAHRSYVIDML
CCCCCCCCCCCEEEEEECCCCCCHHEEEHHHCCEEEEEEECCCCCHHHHHHHHEEEEEEC
NGDALKAIVRREKPDAVVTEIEAINTDALIDLENEGFRIIPNANAVKICMNRIELRLLAS
CCHHHHHHHHCCCCCHHEEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHEEEEEC
ERLKLPTTRYAVANDPGEARRACSDVGYPCLIKPEMSSSGHGHEVVVNPSDVESKFNEAI
CCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHH
KHARGSSRRVIVEEYVDVDKELTVLTYRYPSEGSTVTAVIPPIEHQRPRGVFHYVESWHP
HHCCCCCCEEEEHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCC
SSVSGNIIKTAEDYAVRMVNELGGLGIFGVEIIITRDGRVLFSEVAPRPHDTGLVTLAST
CCCCCCEEECHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEC
DINEFQVHVRSALGLPTPQVKVTTPAAAHVILADNEAWAPRYIGINEALTIPGVQVRLFG
CCHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCEEECCCCEEECCCEEEEEEE
KPNSYRGRRMGVILATGSTVEEAIEKVRKAASIVKVEST
CCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MLGTPLLEGGRRIMILGGGELGKEIAIEAQRMGVEVIVVDRYDMAPAMHVAHRSYVIDML
CCCCCCCCCCCEEEEEECCCCCCHHEEEHHHCCEEEEEEECCCCCHHHHHHHHEEEEEEC
NGDALKAIVRREKPDAVVTEIEAINTDALIDLENEGFRIIPNANAVKICMNRIELRLLAS
CCHHHHHHHHCCCCCHHEEEEEECCCCEEEEECCCCEEEECCCCHHHHHHHHHHEEEEEC
ERLKLPTTRYAVANDPGEARRACSDVGYPCLIKPEMSSSGHGHEVVVNPSDVESKFNEAI
CCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHHH
KHARGSSRRVIVEEYVDVDKELTVLTYRYPSEGSTVTAVIPPIEHQRPRGVFHYVESWHP
HHCCCCCCEEEEHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCC
SSVSGNIIKTAEDYAVRMVNELGGLGIFGVEIIITRDGRVLFSEVAPRPHDTGLVTLAST
CCCCCCEEECHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEECCCCCCCCCCEEEEEEC
DINEFQVHVRSALGLPTPQVKVTTPAAAHVILADNEAWAPRYIGINEALTIPGVQVRLFG
CCHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCEEECCCCEEECCCEEEEEEE
KPNSYRGRRMGVILATGSTVEEAIEKVRKAASIVKVEST
CCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]