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The map label for this gene is pfkA3 [H]

Identifier: 159041220

GI number: 159041220

Start: 678307

End: 679308

Strand: Reverse

Name: pfkA3 [H]

Synonym: Cmaq_0642

Alternate gene names: 159041220

Gene position: 679308-678307 (Counterclockwise)

Preceding gene: 159041223

Following gene: 159041216

Centisome position: 32.7

GC content: 42.61

Gene sequence:

>1002_bases
ATGAGGATTGGAATACTAACCAGCGGTGGGGATGCCCCTGGGTTAAATATTGCAGTGTACTCTTTCATTAAGCTGGCTGA
GAGAAAACATGAGTTATTCGCTGTATTCCATGGTTGGGAGGGGTTTATTGATGGTTCCATTAGGAGAATTGAATCCACTG
ACCTACTACCTTACTTATCCATGGGTGGTACTGCCCTTAAGACATCTAGAAGGAATGTGTTTGATAATGAGGATGACGTC
GAGAAGCTTGTTGAAGCCGTTAATAATTATGGCCTTGACTGTATAGTGGCTATTGGGGGTGATGGATCATTAAAGGCATC
CTATAAGGCCACTGAGTTGGATATAAAGGTTATTCATATTCCTAAGACTATTGATAATGATGTTTTTGGTACTGATTTTA
CTATTGGTTTTGATTCTGCTGTTAATGCTGCTGTTAATGTTACTGAGTCCTTTAAAACCACCTTAATCTCCCATGAGAGG
ATTGGTGTAGTGGAGGTTATGGGTAGGGAGGCTGGATGGGTAGCATTACACACTGGACTAGCAACAATGGCAGACGCAAC
ACTAATACCGGAGTACCCATTAACCATGGACTTCATAGTTAATAAACTAATTGAGGCCTATAAGATAAAGCACTACGCAT
TAGTTGTGACGGCTGAAGGCATTAATGTTAGTGACGGTATTATCACCGCCAATAAGCACCCCACGCCTAATGGAATTGGG
TATAGGTTGGCTGAGTTGATTGAGAAGACGACTGGAATAGAGACTAGGGCTGTTTCACCAGGTCACTCCATAAGGGGTGT
TCAACCGTCTGCGTTTGACCGGGTTCTAGCCGTTAACTACGCTGCTAAGGCCTATGAAGCCGTGGAGAATGAGTTATGTA
ACGTTATGGTTAATTACAGTGAGTCAAGGTATGGTTACGTACCGATACATGAGGTTGTTGGTAAGAATAAGCTTGTTAGC
GGTGGGTGGCTTAGGCTTTATGAGGATTTTTGGGTTAATTAA

Upstream 100 bases:

>100_bases
ACAAGGGTTAAATTAGCTTAACCCAATAACTACCTATGAGTCGCTTAATTACTACTAGAGATTACTGGGTAATTACCATG
AAACACTGGGGTGAGGAACC

Downstream 100 bases:

>100_bases
TCATTGATCCTCCTCAATTACCTTGAAGCCCATTAGTTTAGCCATTTTCTTAAGCTCATCCCTATGATCACCTAGTAAAG
GTACCCTATGCCAACCTGCA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRIGILTSGGDAPGLNIAVYSFIKLAERKHELFAVFHGWEGFIDGSIRRIESTDLLPYLSMGGTALKTSRRNVFDNEDDV
EKLVEAVNNYGLDCIVAIGGDGSLKASYKATELDIKVIHIPKTIDNDVFGTDFTIGFDSAVNAAVNVTESFKTTLISHER
IGVVEVMGREAGWVALHTGLATMADATLIPEYPLTMDFIVNKLIEAYKIKHYALVVTAEGINVSDGIITANKHPTPNGIG
YRLAELIEKTTGIETRAVSPGHSIRGVQPSAFDRVLAVNYAAKAYEAVENELCNVMVNYSESRYGYVPIHEVVGKNKLVS
GGWLRLYEDFWVN

Sequences:

>Translated_333_residues
MRIGILTSGGDAPGLNIAVYSFIKLAERKHELFAVFHGWEGFIDGSIRRIESTDLLPYLSMGGTALKTSRRNVFDNEDDV
EKLVEAVNNYGLDCIVAIGGDGSLKASYKATELDIKVIHIPKTIDNDVFGTDFTIGFDSAVNAAVNVTESFKTTLISHER
IGVVEVMGREAGWVALHTGLATMADATLIPEYPLTMDFIVNKLIEAYKIKHYALVVTAEGINVSDGIITANKHPTPNGIG
YRLAELIEKTTGIETRAVSPGHSIRGVQPSAFDRVLAVNYAAKAYEAVENELCNVMVNYSESRYGYVPIHEVVGKNKLVS
GGWLRLYEDFWVN
>Mature_333_residues
MRIGILTSGGDAPGLNIAVYSFIKLAERKHELFAVFHGWEGFIDGSIRRIESTDLLPYLSMGGTALKTSRRNVFDNEDDV
EKLVEAVNNYGLDCIVAIGGDGSLKASYKATELDIKVIHIPKTIDNDVFGTDFTIGFDSAVNAAVNVTESFKTTLISHER
IGVVEVMGREAGWVALHTGLATMADATLIPEYPLTMDFIVNKLIEAYKIKHYALVVTAEGINVSDGIITANKHPTPNGIG
YRLAELIEKTTGIETRAVSPGHSIRGVQPSAFDRVLAVNYAAKAYEAVENELCNVMVNYSESRYGYVPIHEVVGKNKLVS
GGWLRLYEDFWVN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=32.0512820512821, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI48762920, Length=352, Percent_Identity=29.8295454545455, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI266453768, Length=352, Percent_Identity=30.3977272727273, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI266453748, Length=352, Percent_Identity=30.3977272727273, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4505749, Length=352, Percent_Identity=30.3977272727273, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI266453619, Length=352, Percent_Identity=30.3977272727273, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790350, Length=287, Percent_Identity=34.4947735191638, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25147584, Length=360, Percent_Identity=30.2777777777778, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558788, Length=318, Percent_Identity=28.6163522012579, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI72003601, Length=300, Percent_Identity=25.6666666666667, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321679, Length=349, Percent_Identity=30.0859598853868, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323861, Length=353, Percent_Identity=30.3116147308782, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652337, Length=352, Percent_Identity=29.2613636363636, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17647809, Length=365, Percent_Identity=28.7671232876712, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28573326, Length=352, Percent_Identity=29.5454545454545, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36477; Mature: 36477

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGILTSGGDAPGLNIAVYSFIKLAERKHELFAVFHGWEGFIDGSIRRIESTDLLPYLS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEECCCEEEECCCCCCHHHH
MGGTALKTSRRNVFDNEDDVEKLVEAVNNYGLDCIVAIGGDGSLKASYKATELDIKVIHI
CCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEEEEEEEEE
PKTIDNDVFGTDFTIGFDSAVNAAVNVTESFKTTLISHERIGVVEVMGREAGWVALHTGL
CCCCCCCCCCCEEEEECCCHHHHHEEEHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCH
ATMADATLIPEYPLTMDFIVNKLIEAYKIKHYALVVTAEGINVSDGIITANKHPTPNGIG
HHHHCCEECCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHH
YRLAELIEKTTGIETRAVSPGHSIRGVQPSAFDRVLAVNYAAKAYEAVENELCNVMVNYS
HHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHEEEHHHHHHHHHHHHHHHHEEEEECC
ESRYGYVPIHEVVGKNKLVSGGWLRLYEDFWVN
CCCCCCEEHHHHHCCCCEECCCHHEEHHHHCCC
>Mature Secondary Structure
MRIGILTSGGDAPGLNIAVYSFIKLAERKHELFAVFHGWEGFIDGSIRRIESTDLLPYLS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCEECCCEEEECCCCCCHHHH
MGGTALKTSRRNVFDNEDDVEKLVEAVNNYGLDCIVAIGGDGSLKASYKATELDIKVIHI
CCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEEEEEEEEE
PKTIDNDVFGTDFTIGFDSAVNAAVNVTESFKTTLISHERIGVVEVMGREAGWVALHTGL
CCCCCCCCCCCEEEEECCCHHHHHEEEHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCH
ATMADATLIPEYPLTMDFIVNKLIEAYKIKHYALVVTAEGINVSDGIITANKHPTPNGIG
HHHHCCEECCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHH
YRLAELIEKTTGIETRAVSPGHSIRGVQPSAFDRVLAVNYAAKAYEAVENELCNVMVNYS
HHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHEEEHHHHHHHHHHHHHHHHEEEEECC
ESRYGYVPIHEVVGKNKLVSGGWLRLYEDFWVN
CCCCCCEEHHHHHCCCCEECCCHHEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]