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The map label for this gene is thrS

Identifier: 159041076

GI number: 159041076

Start: 528200

End: 530041

Strand: Reverse

Name: thrS

Synonym: Cmaq_0493

Alternate gene names: 159041076

Gene position: 530041-528200 (Counterclockwise)

Preceding gene: 159041078

Following gene: 159041074

Centisome position: 25.51

GC content: 47.18

Gene sequence:

>1842_bases
ATGAGACTGCTCTTAATACATGCAAGGTCCTTTTCCTTCGAGGTTAGGGATAAGGCGGTTGAGAACCCTGAGCCATTGAC
GGAGGAGTTGAAGAGGGGTAGTGCTGATAATACCTTAGTTGTATTCACTACGGTTGAGGAGAATGATAATGATTCACCAA
GCTTCCTGGAGAGGGTTGCTGATGATGTACTTGATGTTGCAGGTAAGGTTAAGGCATCCTCAGTCTTCCTATACCCATAC
GCCCACCTAAGCCCCAACCTGGCGCCGCCGCCTAAGGCCATAACCATACTTAACGCCCTCTATGAGACTCTTAAGGGTAA
GGCCAGTATACCTGTCTACAGGGCTCCCTTCGGTTGGTATAAGGCCTTTAACATAAGTTGCCTAGGTCACCCATTATCAG
AGTTGAGTAGAACCATTACACCACAGGAGTCTGCCCCACAGAGGAAGCCCTACACTAGGGATTACTACGTTATTGTTTCA
CCGGATGGTTCAATCCATGACCCAGCCAGTTACAGTTTCAGTGAGAGGGATGCTGACTTGAAGGTTCTTGTTGATAAGGA
GGTTTTCAAGAGGGAGCTTGAGGGTAAGGAGCAGCCTAGGTACATTGACTACTGCCTTAAATTCGGCTTCGAGTGGGAGC
CTTTATCGGATGTGGGTCATATGAGGTATGGGCCCTACGCCACTGTTATGCTTGAGCTCCTTGATGATTACTCATACCAA
GTGGCTAAGTCACTGGGCATACCCGTCTTCAAGGTTAAGGGAACCAACATGTTTAGGCTCTCGGATAAGGCGATTAGTGA
GCATGCTGGGTTATTCGGGGAGAGGATGTACATTACCGAGTCTGATGAGGAGCTTGTCATGAGGTACGCCGCATGCTTCC
AGCAATTCGCAATGATTAAGGACTGGGTCTTAAGCTACAGGAACCTACCCTTGGGCATGCTTGAGATTGCTGATAGTTAC
AGGTATGAGCAGCCTGGTGAAACAGTGTTATGCTTTAGGCTTAGACGCTTCTACATGCCTGACCTACACATATTCGTTAA
GGATCTTAAGGAGGCCATGGATGTTGGTTTAAGGCTTCACGCCAAGATCTTTGAGGAGATTAGGAGGATTGGGAGGGATT
ACGTAAGCCTTTACAATGTTAGTAAGGAGTTCTTCGACCAGAATAAGGATTACCTAGTGGAGTTGGCTAGGAGGGAGGGT
AAGCCAATACTGGTTAGGGTCCTTGAGGGGGCGAAGTACTATTGGGTGCTTAACGTTGAGTACCATATTATTGATGAGCT
TAAGAGGCCTAGGGAGATAGCCACATTCCAATTCGACATTGGTAACGCCCAGAGGTTTGGAATCAAGTACAGGGATGAGG
GCAATAACATTAAGTACCCGGTAATAATACATACAGCCATATTAGGCAGTATTGAGAGATTCATATTCGCCCTACTGGAC
ACAGCGGCAATTAATGAGGCTAATGGTAAGGTACCTGCTTTACCAACTTGGATCACCCCAGTTCAAGTCAGGGTTATACC
AGTGTCCAGTGGTTATAATGAGGGGGCCATTGAGTTGGCTAGGAGAATTGAGGAGGCTGGCTTCAGGGTTGAGGTTGATG
ATAGGGATGAGACCGTTGGTAGGAAGATTAGGGATGCTGAAACCCTCTGGGTACCCTACATTGTAGTCTTCGGTGAACGT
GAGGCTAAGACTGGTTCATTAAGCGTTAGGGTTAGGGGTGTGGGTCAGGTTAGTATGAGTATTGAGGAATTACTGAATAG
GCTAAATGAGGAAACCAAGGGTTACCCTAGGAAACCCTTAACAGCACCAATGCTGCTGAGCATAAGGCCACCTTTACCTT
AA

Upstream 100 bases:

>100_bases
AGGGTTTTTAAGGAGCATTAGCTCATAATCCTGGTTTAAGCCAACCATTAAGTGGTGGAACGCACATTTAATTTAAAAAG
TAATCCACGGGGGGTTTGTT

Downstream 100 bases:

>100_bases
GCCATTATTCTATGTATATTCCTGTTGTTTGTATTTTGTGTTTTATGCTTTCCCTCTCCTCCTCTGGGTATTGGTTTATG
TAGTTTATTATTCTTGCCTT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MRLLLIHARSFSFEVRDKAVENPEPLTEELKRGSADNTLVVFTTVEENDNDSPSFLERVADDVLDVAGKVKASSVFLYPY
AHLSPNLAPPPKAITILNALYETLKGKASIPVYRAPFGWYKAFNISCLGHPLSELSRTITPQESAPQRKPYTRDYYVIVS
PDGSIHDPASYSFSERDADLKVLVDKEVFKRELEGKEQPRYIDYCLKFGFEWEPLSDVGHMRYGPYATVMLELLDDYSYQ
VAKSLGIPVFKVKGTNMFRLSDKAISEHAGLFGERMYITESDEELVMRYAACFQQFAMIKDWVLSYRNLPLGMLEIADSY
RYEQPGETVLCFRLRRFYMPDLHIFVKDLKEAMDVGLRLHAKIFEEIRRIGRDYVSLYNVSKEFFDQNKDYLVELARREG
KPILVRVLEGAKYYWVLNVEYHIIDELKRPREIATFQFDIGNAQRFGIKYRDEGNNIKYPVIIHTAILGSIERFIFALLD
TAAINEANGKVPALPTWITPVQVRVIPVSSGYNEGAIELARRIEEAGFRVEVDDRDETVGRKIRDAETLWVPYIVVFGER
EAKTGSLSVRVRGVGQVSMSIEELLNRLNEETKGYPRKPLTAPMLLSIRPPLP

Sequences:

>Translated_613_residues
MRLLLIHARSFSFEVRDKAVENPEPLTEELKRGSADNTLVVFTTVEENDNDSPSFLERVADDVLDVAGKVKASSVFLYPY
AHLSPNLAPPPKAITILNALYETLKGKASIPVYRAPFGWYKAFNISCLGHPLSELSRTITPQESAPQRKPYTRDYYVIVS
PDGSIHDPASYSFSERDADLKVLVDKEVFKRELEGKEQPRYIDYCLKFGFEWEPLSDVGHMRYGPYATVMLELLDDYSYQ
VAKSLGIPVFKVKGTNMFRLSDKAISEHAGLFGERMYITESDEELVMRYAACFQQFAMIKDWVLSYRNLPLGMLEIADSY
RYEQPGETVLCFRLRRFYMPDLHIFVKDLKEAMDVGLRLHAKIFEEIRRIGRDYVSLYNVSKEFFDQNKDYLVELARREG
KPILVRVLEGAKYYWVLNVEYHIIDELKRPREIATFQFDIGNAQRFGIKYRDEGNNIKYPVIIHTAILGSIERFIFALLD
TAAINEANGKVPALPTWITPVQVRVIPVSSGYNEGAIELARRIEEAGFRVEVDDRDETVGRKIRDAETLWVPYIVVFGER
EAKTGSLSVRVRGVGQVSMSIEELLNRLNEETKGYPRKPLTAPMLLSIRPPLP
>Mature_613_residues
MRLLLIHARSFSFEVRDKAVENPEPLTEELKRGSADNTLVVFTTVEENDNDSPSFLERVADDVLDVAGKVKASSVFLYPY
AHLSPNLAPPPKAITILNALYETLKGKASIPVYRAPFGWYKAFNISCLGHPLSELSRTITPQESAPQRKPYTRDYYVIVS
PDGSIHDPASYSFSERDADLKVLVDKEVFKRELEGKEQPRYIDYCLKFGFEWEPLSDVGHMRYGPYATVMLELLDDYSYQ
VAKSLGIPVFKVKGTNMFRLSDKAISEHAGLFGERMYITESDEELVMRYAACFQQFAMIKDWVLSYRNLPLGMLEIADSY
RYEQPGETVLCFRLRRFYMPDLHIFVKDLKEAMDVGLRLHAKIFEEIRRIGRDYVSLYNVSKEFFDQNKDYLVELARREG
KPILVRVLEGAKYYWVLNVEYHIIDELKRPREIATFQFDIGNAQRFGIKYRDEGNNIKYPVIIHTAILGSIERFIFALLD
TAAINEANGKVPALPTWITPVQVRVIPVSSGYNEGAIELARRIEEAGFRVEVDDRDETVGRKIRDAETLWVPYIVVFGER
EAKTGSLSVRVRGVGQVSMSIEELLNRLNEETKGYPRKPLTAPMLLSIRPPLP

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI20070344, Length=303, Percent_Identity=27.3927392739274, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI38202255, Length=346, Percent_Identity=28.9017341040462, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38570111, Length=349, Percent_Identity=27.2206303724928, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788013, Length=388, Percent_Identity=29.8969072164948, Blast_Score=145, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71984184, Length=328, Percent_Identity=27.1341463414634, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71984192, Length=328, Percent_Identity=27.1341463414634, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322112, Length=464, Percent_Identity=25.8620689655172, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322654, Length=308, Percent_Identity=25, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24583839, Length=346, Percent_Identity=28.9017341040462, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583842, Length=346, Percent_Identity=28.9017341040462, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574141, Length=346, Percent_Identity=28.9017341040462, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_CALMQ (A8MBZ5)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540328.1
- ProteinModelPortal:   A8MBZ5
- GeneID:   5709874
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0493
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- BioCyc:   CMAQ397948:CMAQ_0493-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 70256; Mature: 70256

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLLIHARSFSFEVRDKAVENPEPLTEELKRGSADNTLVVFTTVEENDNDSPSFLERVA
CEEEEEEECCCCEEEHHHCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHH
DDVLDVAGKVKASSVFLYPYAHLSPNLAPPPKAITILNALYETLKGKASIPVYRAPFGWY
HHHHHHHCCCEECEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCH
KAFNISCLGHPLSELSRTITPQESAPQRKPYTRDYYVIVSPDGSIHDPASYSFSERDADL
HEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
KVLVDKEVFKRELEGKEQPRYIDYCLKFGFEWEPLSDVGHMRYGPYATVMLELLDDYSYQ
EEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAKSLGIPVFKVKGTNMFRLSDKAISEHAGLFGERMYITESDEELVMRYAACFQQFAMIK
HHHHCCCCEEEECCCEEEEECHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
DWVLSYRNLPLGMLEIADSYRYEQPGETVLCFRLRRFYMPDLHIFVKDLKEAMDVGLRLH
HHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHH
AKIFEEIRRIGRDYVSLYNVSKEFFDQNKDYLVELARREGKPILVRVLEGAKYYWVLNVE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCEEEEEEEEE
YHIIDELKRPREIATFQFDIGNAQRFGIKYRDEGNNIKYPVIIHTAILGSIERFIFALLD
HHHHHHHCCCCEEEEEEEECCCCEEECEEEECCCCCEEEEHEEHHHHHHHHHHHHHHHHH
TAAINEANGKVPALPTWITPVQVRVIPVSSGYNEGAIELARRIEEAGFRVEVDDRDETVG
HHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHC
RKIRDAETLWVPYIVVFGEREAKTGSLSVRVRGVGQVSMSIEELLNRLNEETKGYPRKPL
CHHHCCCEEEEEEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
TAPMLLSIRPPLP
CCCEEEEECCCCC
>Mature Secondary Structure
MRLLLIHARSFSFEVRDKAVENPEPLTEELKRGSADNTLVVFTTVEENDNDSPSFLERVA
CEEEEEEECCCCEEEHHHCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHH
DDVLDVAGKVKASSVFLYPYAHLSPNLAPPPKAITILNALYETLKGKASIPVYRAPFGWY
HHHHHHHCCCEECEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCH
KAFNISCLGHPLSELSRTITPQESAPQRKPYTRDYYVIVSPDGSIHDPASYSFSERDADL
HEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCE
KVLVDKEVFKRELEGKEQPRYIDYCLKFGFEWEPLSDVGHMRYGPYATVMLELLDDYSYQ
EEEECHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAKSLGIPVFKVKGTNMFRLSDKAISEHAGLFGERMYITESDEELVMRYAACFQQFAMIK
HHHHCCCCEEEECCCEEEEECHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
DWVLSYRNLPLGMLEIADSYRYEQPGETVLCFRLRRFYMPDLHIFVKDLKEAMDVGLRLH
HHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHH
AKIFEEIRRIGRDYVSLYNVSKEFFDQNKDYLVELARREGKPILVRVLEGAKYYWVLNVE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCEEEEEEEEE
YHIIDELKRPREIATFQFDIGNAQRFGIKYRDEGNNIKYPVIIHTAILGSIERFIFALLD
HHHHHHHCCCCEEEEEEEECCCCEEECEEEECCCCCEEEEHEEHHHHHHHHHHHHHHHHH
TAAINEANGKVPALPTWITPVQVRVIPVSSGYNEGAIELARRIEEAGFRVEVDDRDETVG
HHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHC
RKIRDAETLWVPYIVVFGEREAKTGSLSVRVRGVGQVSMSIEELLNRLNEETKGYPRKPL
CHHHCCCEEEEEEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCC
TAPMLLSIRPPLP
CCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA