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The map label for this gene is rtcA

Identifier: 159040881

GI number: 159040881

Start: 332791

End: 333855

Strand: Reverse

Name: rtcA

Synonym: Cmaq_0295

Alternate gene names: 159040881

Gene position: 333855-332791 (Counterclockwise)

Preceding gene: 159040882

Following gene: 159040880

Centisome position: 16.07

GC content: 47.79

Gene sequence:

>1065_bases
ATGATAACCATAGACGGCTCAATGGGTGAGGGAGGCGGACAGATACTGAGGACTGCCTTAGCCCTATCCATAATCACTGG
TAAACCCTTCAGGATAATTAACATTAGGGCTAGGAGAAGTAATCCAGGGTTGCAGCCCCAGCACCTGGCCAGTGTCATGG
CTGCTGCAAGGATCAGTAACGCTAAGGTTGATGGAGCATATAAGGGGTCCTTAAGCCTAACCTTCGAACCAGGGGATGTT
AAATGCGGTGACTACAGTATTGATATTGGAACTGCAGGGAGCATAAGCCTAGTGCTCCAAACCCTACTACCAGTATTAGC
CGTAGTGAACTGTGGTGAAGTCACCCTTGACATCACCGGTGGTACAGATGTACCTAAGGCCCCTCCCATAGATTACGTAC
GCTTCGTACTGGCTCACAACCTATCCCTAATGGGTGTGGGGATTAAAGTGGAATTAATTAGGAGGGGTCATTACCCAAGG
GGTGGCGGTAAGGTTAAGGTTACCGTTAAGCCAGCTGGTAGACTTAAGCCAATCAACATAACTGAGCTTGGTGAATTAAA
AGGGATCTGGGGGTTATCACATGCAGTTAGGTTACCAAGCCATGTAGCGGTTAGGCAGGCTAAGGCCGCTGAGGATTATT
TAAGTAAACTAGGCCTAAAACCCAGCATTAGCCTCGAGTACTATGAGCAGGGTAAGGATCCCCACCTAGGGCCTGGTTCA
GGAATAACACTATGGGCTGAGTCCACTAATGGGCAGAGGATTGGAGCTGACTCACTGGGTGAACGCGGTAAACCAGCGGA
GGATGTTGGTAGGGAGGCTGCAGAGGCCCTTGCGGCAGTAATTAATGCGGGGGCGGCCTTCGATGACCACATGGGTGATA
TGCTTATCCCATTCCTAGCCCTAGCTGAAGGCAGATCAGAGTACACTGTGGTAAACTTAACATCACACCTATCAACGAAT
ATAAGTATTGTTAAACTATTCCTAAATGCCAATATAGAAACCATGAACTATAATAAGAAAGTTAAAGTAACCATTAACCC
AATTACCGCGCCAAGGAAGCCTTAA

Upstream 100 bases:

>100_bases
AGCAATATTAGGGTGCCCTTAAATAAACTTAGGCTTAATTAAAGTGATGAACACTTCAGTACTTAGGGTTTATTAACCAA
GCCCAGTGGGTATTATGGGC

Downstream 100 bases:

>100_bases
ACCATCATAAATACACGTCAAGTAATGCGTAAAATTTACCTACAGCAGGATAGTAGGGAATTTAATAATAAGTAATCCTG
CATTCCTGGTTTAATGTTGT

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MITIDGSMGEGGGQILRTALALSIITGKPFRIINIRARRSNPGLQPQHLASVMAAARISNAKVDGAYKGSLSLTFEPGDV
KCGDYSIDIGTAGSISLVLQTLLPVLAVVNCGEVTLDITGGTDVPKAPPIDYVRFVLAHNLSLMGVGIKVELIRRGHYPR
GGGKVKVTVKPAGRLKPINITELGELKGIWGLSHAVRLPSHVAVRQAKAAEDYLSKLGLKPSISLEYYEQGKDPHLGPGS
GITLWAESTNGQRIGADSLGERGKPAEDVGREAAEALAAVINAGAAFDDHMGDMLIPFLALAEGRSEYTVVNLTSHLSTN
ISIVKLFLNANIETMNYNKKVKVTINPITAPRKP

Sequences:

>Translated_354_residues
MITIDGSMGEGGGQILRTALALSIITGKPFRIINIRARRSNPGLQPQHLASVMAAARISNAKVDGAYKGSLSLTFEPGDV
KCGDYSIDIGTAGSISLVLQTLLPVLAVVNCGEVTLDITGGTDVPKAPPIDYVRFVLAHNLSLMGVGIKVELIRRGHYPR
GGGKVKVTVKPAGRLKPINITELGELKGIWGLSHAVRLPSHVAVRQAKAAEDYLSKLGLKPSISLEYYEQGKDPHLGPGS
GITLWAESTNGQRIGADSLGERGKPAEDVGREAAEALAAVINAGAAFDDHMGDMLIPFLALAEGRSEYTVVNLTSHLSTN
ISIVKLFLNANIETMNYNKKVKVTINPITAPRKP
>Mature_354_residues
MITIDGSMGEGGGQILRTALALSIITGKPFRIINIRARRSNPGLQPQHLASVMAAARISNAKVDGAYKGSLSLTFEPGDV
KCGDYSIDIGTAGSISLVLQTLLPVLAVVNCGEVTLDITGGTDVPKAPPIDYVRFVLAHNLSLMGVGIKVELIRRGHYPR
GGGKVKVTVKPAGRLKPINITELGELKGIWGLSHAVRLPSHVAVRQAKAAEDYLSKLGLKPSISLEYYEQGKDPHLGPGS
GITLWAESTNGQRIGADSLGERGKPAEDVGREAAEALAAVINAGAAFDDHMGDMLIPFLALAEGRSEYTVVNLTSHLSTN
ISIVKLFLNANIETMNYNKKVKVTINPITAPRKP

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=333, Percent_Identity=38.1381381381381, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI195927044, Length=346, Percent_Identity=36.7052023121387, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI157426877, Length=313, Percent_Identity=28.4345047923323, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI48994937, Length=348, Percent_Identity=39.367816091954, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32564710, Length=348, Percent_Identity=26.4367816091954, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324564, Length=297, Percent_Identity=23.5690235690236, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI18543269, Length=326, Percent_Identity=35.5828220858896, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI18860065, Length=334, Percent_Identity=25.4491017964072, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_CALMQ (A8MAV7)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001540133.1
- ProteinModelPortal:   A8MAV7
- SMR:   A8MAV7
- GeneID:   5708793
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0295
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- ProtClustDB:   PRK04204
- BioCyc:   CMAQ397948:CMAQ_0295-MONOMER
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 37551; Mature: 37551

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 316-316 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIDGSMGEGGGQILRTALALSIITGKPFRIINIRARRSNPGLQPQHLASVMAAARISN
CEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCC
AKVDGAYKGSLSLTFEPGDVKCGDYSIDIGTAGSISLVLQTLLPVLAVVNCGEVTLDITG
CCCCCEEECEEEEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
GTDVPKAPPIDYVRFVLAHNLSLMGVGIKVELIRRGHYPRGGGKVKVTVKPAGRLKPINI
CCCCCCCCCHHHHHHHHHCCCEEEEECEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCC
TELGELKGIWGLSHAVRLPSHVAVRQAKAAEDYLSKLGLKPSISLEYYEQGKDPHLGPGS
HHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCC
GITLWAESTNGQRIGADSLGERGKPAEDVGREAAEALAAVINAGAAFDDHMGDMLIPFLA
CEEEEEECCCCCEECCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAEGRSEYTVVNLTSHLSTNISIVKLFLNANIETMNYNKKVKVTINPITAPRKP
HHCCCCCEEEEEEHHHCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MITIDGSMGEGGGQILRTALALSIITGKPFRIINIRARRSNPGLQPQHLASVMAAARISN
CEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCC
AKVDGAYKGSLSLTFEPGDVKCGDYSIDIGTAGSISLVLQTLLPVLAVVNCGEVTLDITG
CCCCCEEECEEEEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEC
GTDVPKAPPIDYVRFVLAHNLSLMGVGIKVELIRRGHYPRGGGKVKVTVKPAGRLKPINI
CCCCCCCCCHHHHHHHHHCCCEEEEECEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCC
TELGELKGIWGLSHAVRLPSHVAVRQAKAAEDYLSKLGLKPSISLEYYEQGKDPHLGPGS
HHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCC
GITLWAESTNGQRIGADSLGERGKPAEDVGREAAEALAAVINAGAAFDDHMGDMLIPFLA
CEEEEEECCCCCEECCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAEGRSEYTVVNLTSHLSTNISIVKLFLNANIETMNYNKKVKVTINPITAPRKP
HHCCCCCEEEEEEHHHCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA