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The map label for this gene is prf1 [H]

Identifier: 159040850

GI number: 159040850

Start: 301962

End: 303134

Strand: Reverse

Name: prf1 [H]

Synonym: Cmaq_0264

Alternate gene names: 159040850

Gene position: 303134-301962 (Counterclockwise)

Preceding gene: 159040852

Following gene: 159040848

Centisome position: 14.59

GC content: 43.56

Gene sequence:

>1173_bases
TTGTTAAAGGTTGATGGTGTCCTAGGTGGACGCACTGGAATAACAGATGGGGAGGAGTTGAAGATAATGATTCAATTACT
GAAGAAATACAGGGGTTATGCAACAACCTTAATATCACTCTACATTAATGGGGATAGGCCAATACCAGATGTCTTAAACA
TGCTTAGGAATGAGTGGTCGGTTGCAAGTAACATTAAGGATAAGACAACTAGGACTCATGTTCAAGATGCCTTGGAGAGG
ATAATTAACGCATTAAAGGGTACGGCTAAAGCACCACCCAATGGTTTAGCGGTATTCGGAGGCTTCCACATGGAGAAGCC
GGGTAGCTATAAGTGGGTCATGTACGCTATAATACCCCCATATAAGATAAGTACGTATAAGTACGTCTGCGATACTCGCT
TCCACACTGAGCTCCTTGAGGATATGATATCGGCATCATCATCAGTGTTCGGGATAATAGTTATTGAGAGGGGGGAGGCT
GTCATAGCGTTACTTAAGGGTAGTTACTGGGAGATAGTGGATAAGGTCGAGTTCTTCGTACCCAATAAGCATCATGCAGG
TGGTCAATCAGCCTTAAGGTTTAAGAGACAGACTGAACACCTGGCTGAGACATTCTATAAGCTCGTTGCCGAGGATGCTA
ATAAGATACTGCTTCAAATACCGAACCTTAAGGGTATTATTGTAGCTGGCCCAGGGCCAACTAAGGAGGACTTCCTTAAG
GAGGGTGGCTTAGATAGTAGACTCATGGATAAGATAATTGCAATAGTACCCGCCTGCTGTGCAGATATTAGTGGTGTATT
GGAGGCTATTAAGGCCTCTGAGGATAAGCTTAAGGAGACTGAGTATGTTAAGGCTAAGAAACTGATGGAGGAGGTAATGT
ATATTGCCGTTAAGAAGCCTGACTACATAGTCTATGGTAGGGATAACGTGTTGAACGCCATTGAGAAGGGTATTGCCAAG
GTTGTGTTAATAAGTGAGGATATTGGTGAGGATGAGATCTTTAAATTAACAACAATGCTTAGGGGTAAGAAAATAGACCT
AGTAGTCATACCGAATAGCGTTGAGGAGAGCCTAACCCTAAGCAAAACCTTCGGCGGATACGTGGCCATATTGGCTACCC
CATCATGGATAATTGAGGAGTGGGAAAACGCCAGTCAAAGCTTAACTCAGTAG

Upstream 100 bases:

>100_bases
TTAACCCCACGTTACTTACCGTCTAATTAGGCTTAATAAATCATTCGATCAGTAACCTGTAGGTGTTTTTACATGCGGGG
AAAAGGTTTATAAAGCATTA

Downstream 100 bases:

>100_bases
ACTTAACTTTAAGCCACACCGAGTTGCTTATCTAATGCAACCTTATACCTAATCCACCATTCAGTGTAATCACTCTTACT
CTCAAATACCCACTCCTTGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLKVDGVLGGRTGITDGEELKIMIQLLKKYRGYATTLISLYINGDRPIPDVLNMLRNEWSVASNIKDKTTRTHVQDALER
IINALKGTAKAPPNGLAVFGGFHMEKPGSYKWVMYAIIPPYKISTYKYVCDTRFHTELLEDMISASSSVFGIIVIERGEA
VIALLKGSYWEIVDKVEFFVPNKHHAGGQSALRFKRQTEHLAETFYKLVAEDANKILLQIPNLKGIIVAGPGPTKEDFLK
EGGLDSRLMDKIIAIVPACCADISGVLEAIKASEDKLKETEYVKAKKLMEEVMYIAVKKPDYIVYGRDNVLNAIEKGIAK
VVLISEDIGEDEIFKLTTMLRGKKIDLVVIPNSVEESLTLSKTFGGYVAILATPSWIIEEWENASQSLTQ

Sequences:

>Translated_390_residues
MLKVDGVLGGRTGITDGEELKIMIQLLKKYRGYATTLISLYINGDRPIPDVLNMLRNEWSVASNIKDKTTRTHVQDALER
IINALKGTAKAPPNGLAVFGGFHMEKPGSYKWVMYAIIPPYKISTYKYVCDTRFHTELLEDMISASSSVFGIIVIERGEA
VIALLKGSYWEIVDKVEFFVPNKHHAGGQSALRFKRQTEHLAETFYKLVAEDANKILLQIPNLKGIIVAGPGPTKEDFLK
EGGLDSRLMDKIIAIVPACCADISGVLEAIKASEDKLKETEYVKAKKLMEEVMYIAVKKPDYIVYGRDNVLNAIEKGIAK
VVLISEDIGEDEIFKLTTMLRGKKIDLVVIPNSVEESLTLSKTFGGYVAILATPSWIIEEWENASQSLTQ
>Mature_390_residues
MLKVDGVLGGRTGITDGEELKIMIQLLKKYRGYATTLISLYINGDRPIPDVLNMLRNEWSVASNIKDKTTRTHVQDALER
IINALKGTAKAPPNGLAVFGGFHMEKPGSYKWVMYAIIPPYKISTYKYVCDTRFHTELLEDMISASSSVFGIIVIERGEA
VIALLKGSYWEIVDKVEFFVPNKHHAGGQSALRFKRQTEHLAETFYKLVAEDANKILLQIPNLKGIIVAGPGPTKEDFLK
EGGLDSRLMDKIIAIVPACCADISGVLEAIKASEDKLKETEYVKAKKLMEEVMYIAVKKPDYIVYGRDNVLNAIEKGIAK
VVLISEDIGEDEIFKLTTMLRGKKIDLVVIPNSVEESLTLSKTFGGYVAILATPSWIIEEWENASQSLTQ

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=312, Percent_Identity=31.7307692307692, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI72000368, Length=314, Percent_Identity=31.2101910828025, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319619, Length=312, Percent_Identity=33.974358974359, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28574632, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21356433, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24667392, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24667388, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24667380, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24667384, Length=312, Percent_Identity=31.4102564102564, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 43389; Mature: 43389

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVDGVLGGRTGITDGEELKIMIQLLKKYRGYATTLISLYINGDRPIPDVLNMLRNEWS
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHHH
VASNIKDKTTRTHVQDALERIINALKGTAKAPPNGLAVFGGFHMEKPGSYKWVMYAIIPP
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECEECCCCCCCEEEEEEEECC
YKISTYKYVCDTRFHTELLEDMISASSSVFGIIVIERGEAVIALLKGSYWEIVDKVEFFV
EEECEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCHHHHHHHHHHCC
PNKHHAGGQSALRFKRQTEHLAETFYKLVAEDANKILLQIPNLKGIIVAGPGPTKEDFLK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHH
EGGLDSRLMDKIIAIVPACCADISGVLEAIKASEDKLKETEYVKAKKLMEEVMYIAVKKP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCC
DYIVYGRDNVLNAIEKGIAKVVLISEDIGEDEIFKLTTMLRGKKIDLVVIPNSVEESLTL
CEEEECCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
SKTFGGYVAILATPSWIIEEWENASQSLTQ
HHHCCCEEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKVDGVLGGRTGITDGEELKIMIQLLKKYRGYATTLISLYINGDRPIPDVLNMLRNEWS
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHHH
VASNIKDKTTRTHVQDALERIINALKGTAKAPPNGLAVFGGFHMEKPGSYKWVMYAIIPP
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECEECCCCCCCEEEEEEEECC
YKISTYKYVCDTRFHTELLEDMISASSSVFGIIVIERGEAVIALLKGSYWEIVDKVEFFV
EEECEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCHHHHHHHHHHCC
PNKHHAGGQSALRFKRQTEHLAETFYKLVAEDANKILLQIPNLKGIIVAGPGPTKEDFLK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHH
EGGLDSRLMDKIIAIVPACCADISGVLEAIKASEDKLKETEYVKAKKLMEEVMYIAVKKP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCC
DYIVYGRDNVLNAIEKGIAKVVLISEDIGEDEIFKLTTMLRGKKIDLVVIPNSVEESLTL
CEEEECCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
SKTFGGYVAILATPSWIIEEWENASQSLTQ
HHHCCCEEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]