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The map label for this gene is metG

Identifier: 159040724

GI number: 159040724

Start: 160628

End: 162376

Strand: Reverse

Name: metG

Synonym: Cmaq_0136

Alternate gene names: 159040724

Gene position: 162376-160628 (Counterclockwise)

Preceding gene: 159040728

Following gene: 159040723

Centisome position: 7.82

GC content: 43.68

Gene sequence:

>1749_bases
GTGCTTGATAGGCGAATCTTCAGTGACCCCAATGGAAATGAGGTAAGGTGGGTTGTGGGCTCAGCATGGCCATATATTTA
CGCCGTCCCACACTTAGGTAATCTAATAGGTTCACTATTATCCGCTGATGTATTCACAAGGTATCTTAAGTTGAAGGGTT
ATGATGTCGTCTTTGTCACTGGTAGTGATGAGCATGGTACGCCAATTGAGGTTGAGGCCATTAAACTGGGTATTGAACCA
AGGCAATTAACGGATAGGATGCATGAAATCATTGTTAAGTTACTTAAGCTTTGGGGTATTGAACCCGATAACTACACTAG
GACTGAGTCGGAGGTTCATAAGTGGTATGTTAGGGATGCCTTCACTAAAATCTACAATAATGGCTACATATTCACTAAGG
ATGATGAATTACCCTACTGCCCAAGGGATAAGATATTCCTACCTGATAGGTTCGTGATAGGTACATGCCCATACTGCGGT
TACCCCTACGCAAGGGGTGATCAGTGTGAGAACTGCGGTAGGTTGCTTGAACCAAGAATGTTAATTAACCCCAAGTGCGC
GATATGTGGTTCAACACCAGAGTGGAGATTAACCAGGCACTGGTACCTCGACTTAAGGAGGCTTGAGGATAGGATTAGGA
GCTACATTGAGGGGAATAGTGCGCTTCCAGATAATGCGAAGCAAATGAGCCTGGGGATACTTAAGGAGGGTTTAAGGCCT
AGGGCAATAACAAGGGATAATAAGTGGGGTATCCCAGCCCCATTCCCTGGAGCTGAGGGTAAGACTATTTACGTCTGGTT
TGAGGCAGTTTTAGGCTATGTCTCAGCAACCATAGAGTACTTTAGGAGGCTTGGCAGGGAGGATGATTGGAGGAGGTTCT
GGTTTAATAAGGGGACTAGAGTTGTATTCTTCATAGGTAAGGATAACATACCGTTCCACACAATAATATTCCCAGCACTA
CTTATGGCTACGGGGGAGGATTACGTAATGCCTTGGACAACGTCATCAACCGAGTACTTGATTTTCGAAGGCAAGAAGTT
CTCAAAGAGCCAGAGGGTTGGGATTTGGGCTGATGAAGCAATAGCCCTACTCCCAGCGGACTACTGGAGGTTCTACCTCA
TTTACAATAGGCCTGAGCAGAGGGATAGTAACTTCACGTGGGATTCATTCCTAGATGTAGTTAACTCAATTATGAATGAC
ACAGTGGGTAACTTCATACACAGGGTATTGACACTGGCTAAGAGGAGGTGGGGTACTGTACCCGGTGACGTTAAGATGAT
TGAGCAGGACCAGGAGATTTACGGTAAGGTTCTTGAAATCCTCAACACTGTTGAGGAGGATTATGAGAGAATAATGCTAA
AGGATGCGGTATCCCAGTCTATTGAAATAGCAAGGATAGGCAATAAGTACCTTAATGAGAGGCAACCATGGAGATTAAGT
GAAGTGGAATTCAATAGTGCAATATATATGCTCATGAGCATTGTTAAGACATTATCAATAACACTTGCACCAGTAATACC
CTTCAGTATTAATGAGTTATGGAGAATGATGGGTTACAGTAATGGCTTAAGATGGGGGGATGCTAGGAAGCCTATTGAAC
AGGGATTACGGCTAAGTGAACCAAAGCCATTATTCAGGAAAATAAGTAAGGAGGAGTTGAATGTAATGCTTAAGAAGCTT
GATGAAATAAGGGACATTAAGGATAAGGGTAAATACCCGTGGGAGCAAGCCTACTTACCTAATCCTTAA

Upstream 100 bases:

>100_bases
AATATCAGTTTAAAAACCGAACTCACCAGTGCTCTAAGTATTGGCTCAGGGTAATGGACTTAAGCAGCCTAATTTATTGT
AATGGAAAAGCTTATTAAGA

Downstream 100 bases:

>100_bases
GTCAAATGCGCATTAACGGTAAATATTTATAACCCAGGTAAACTCAACGCCTGTGTCTAATCAGCAGAATAATGATGAGA
TAGGGCCTGTTGAAGTTAAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MLDRRIFSDPNGNEVRWVVGSAWPYIYAVPHLGNLIGSLLSADVFTRYLKLKGYDVVFVTGSDEHGTPIEVEAIKLGIEP
RQLTDRMHEIIVKLLKLWGIEPDNYTRTESEVHKWYVRDAFTKIYNNGYIFTKDDELPYCPRDKIFLPDRFVIGTCPYCG
YPYARGDQCENCGRLLEPRMLINPKCAICGSTPEWRLTRHWYLDLRRLEDRIRSYIEGNSALPDNAKQMSLGILKEGLRP
RAITRDNKWGIPAPFPGAEGKTIYVWFEAVLGYVSATIEYFRRLGREDDWRRFWFNKGTRVVFFIGKDNIPFHTIIFPAL
LMATGEDYVMPWTTSSTEYLIFEGKKFSKSQRVGIWADEAIALLPADYWRFYLIYNRPEQRDSNFTWDSFLDVVNSIMND
TVGNFIHRVLTLAKRRWGTVPGDVKMIEQDQEIYGKVLEILNTVEEDYERIMLKDAVSQSIEIARIGNKYLNERQPWRLS
EVEFNSAIYMLMSIVKTLSITLAPVIPFSINELWRMMGYSNGLRWGDARKPIEQGLRLSEPKPLFRKISKEELNVMLKKL
DEIRDIKDKGKYPWEQAYLPNP

Sequences:

>Translated_582_residues
MLDRRIFSDPNGNEVRWVVGSAWPYIYAVPHLGNLIGSLLSADVFTRYLKLKGYDVVFVTGSDEHGTPIEVEAIKLGIEP
RQLTDRMHEIIVKLLKLWGIEPDNYTRTESEVHKWYVRDAFTKIYNNGYIFTKDDELPYCPRDKIFLPDRFVIGTCPYCG
YPYARGDQCENCGRLLEPRMLINPKCAICGSTPEWRLTRHWYLDLRRLEDRIRSYIEGNSALPDNAKQMSLGILKEGLRP
RAITRDNKWGIPAPFPGAEGKTIYVWFEAVLGYVSATIEYFRRLGREDDWRRFWFNKGTRVVFFIGKDNIPFHTIIFPAL
LMATGEDYVMPWTTSSTEYLIFEGKKFSKSQRVGIWADEAIALLPADYWRFYLIYNRPEQRDSNFTWDSFLDVVNSIMND
TVGNFIHRVLTLAKRRWGTVPGDVKMIEQDQEIYGKVLEILNTVEEDYERIMLKDAVSQSIEIARIGNKYLNERQPWRLS
EVEFNSAIYMLMSIVKTLSITLAPVIPFSINELWRMMGYSNGLRWGDARKPIEQGLRLSEPKPLFRKISKEELNVMLKKL
DEIRDIKDKGKYPWEQAYLPNP
>Mature_582_residues
MLDRRIFSDPNGNEVRWVVGSAWPYIYAVPHLGNLIGSLLSADVFTRYLKLKGYDVVFVTGSDEHGTPIEVEAIKLGIEP
RQLTDRMHEIIVKLLKLWGIEPDNYTRTESEVHKWYVRDAFTKIYNNGYIFTKDDELPYCPRDKIFLPDRFVIGTCPYCG
YPYARGDQCENCGRLLEPRMLINPKCAICGSTPEWRLTRHWYLDLRRLEDRIRSYIEGNSALPDNAKQMSLGILKEGLRP
RAITRDNKWGIPAPFPGAEGKTIYVWFEAVLGYVSATIEYFRRLGREDDWRRFWFNKGTRVVFFIGKDNIPFHTIIFPAL
LMATGEDYVMPWTTSSTEYLIFEGKKFSKSQRVGIWADEAIALLPADYWRFYLIYNRPEQRDSNFTWDSFLDVVNSIMND
TVGNFIHRVLTLAKRRWGTVPGDVKMIEQDQEIYGKVLEILNTVEEDYERIMLKDAVSQSIEIARIGNKYLNERQPWRLS
EVEFNSAIYMLMSIVKTLSITLAPVIPFSINELWRMMGYSNGLRWGDARKPIEQGLRLSEPKPLFRKISKEELNVMLKKL
DEIRDIKDKGKYPWEQAYLPNP

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=566, Percent_Identity=33.9222614840989, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI24308436, Length=529, Percent_Identity=23.4404536862004, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI1788432, Length=556, Percent_Identity=27.6978417266187, Blast_Score=227, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17540794, Length=585, Percent_Identity=34.5299145299145, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI212645096, Length=507, Percent_Identity=24.8520710059172, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=23.5872235872236, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321704, Length=567, Percent_Identity=35.2733686067019, Blast_Score=357, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6321610, Length=525, Percent_Identity=24.1904761904762, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24655311, Length=591, Percent_Identity=35.3637901861252, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21358483, Length=510, Percent_Identity=22.9411764705882, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CALMQ (A8MA54)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001539976.1
- ProteinModelPortal:   A8MA54
- SMR:   A8MA54
- GeneID:   5709876
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0136
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   CMAQ397948:CMAQ_0136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 67958; Mature: 67958

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRRIFSDPNGNEVRWVVGSAWPYIYAVPHLGNLIGSLLSADVFTRYLKLKGYDVVFVT
CCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GSDEHGTPIEVEAIKLGIEPRQLTDRMHEIIVKLLKLWGIEPDNYTRTESEVHKWYVRDA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FTKIYNNGYIFTKDDELPYCPRDKIFLPDRFVIGTCPYCGYPYARGDQCENCGRLLEPRM
HHHHHCCCEEEEECCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCE
LINPKCAICGSTPEWRLTRHWYLDLRRLEDRIRSYIEGNSALPDNAKQMSLGILKEGLRP
EECCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
RAITRDNKWGIPAPFPGAEGKTIYVWFEAVLGYVSATIEYFRRLGREDDWRRFWFNKGTR
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCE
VVFFIGKDNIPFHTIIFPALLMATGEDYVMPWTTSSTEYLIFEGKKFSKSQRVGIWADEA
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEECCCH
IALLPADYWRFYLIYNRPEQRDSNFTWDSFLDVVNSIMNDTVGNFIHRVLTLAKRRWGTV
HEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDVKMIEQDQEIYGKVLEILNTVEEDYERIMLKDAVSQSIEIARIGNKYLNERQPWRLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCEEC
EVEFNSAIYMLMSIVKTLSITLAPVIPFSINELWRMMGYSNGLRWGDARKPIEQGLRLSE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
PKPLFRKISKEELNVMLKKLDEIRDIKDKGKYPWEQAYLPNP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MLDRRIFSDPNGNEVRWVVGSAWPYIYAVPHLGNLIGSLLSADVFTRYLKLKGYDVVFVT
CCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GSDEHGTPIEVEAIKLGIEPRQLTDRMHEIIVKLLKLWGIEPDNYTRTESEVHKWYVRDA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FTKIYNNGYIFTKDDELPYCPRDKIFLPDRFVIGTCPYCGYPYARGDQCENCGRLLEPRM
HHHHHCCCEEEEECCCCCCCCCCCEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCE
LINPKCAICGSTPEWRLTRHWYLDLRRLEDRIRSYIEGNSALPDNAKQMSLGILKEGLRP
EECCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
RAITRDNKWGIPAPFPGAEGKTIYVWFEAVLGYVSATIEYFRRLGREDDWRRFWFNKGTR
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCE
VVFFIGKDNIPFHTIIFPALLMATGEDYVMPWTTSSTEYLIFEGKKFSKSQRVGIWADEA
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEECCCH
IALLPADYWRFYLIYNRPEQRDSNFTWDSFLDVVNSIMNDTVGNFIHRVLTLAKRRWGTV
HEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDVKMIEQDQEIYGKVLEILNTVEEDYERIMLKDAVSQSIEIARIGNKYLNERQPWRLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHCCCCCCEEC
EVEFNSAIYMLMSIVKTLSITLAPVIPFSINELWRMMGYSNGLRWGDARKPIEQGLRLSE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
PKPLFRKISKEELNVMLKKLDEIRDIKDKGKYPWEQAYLPNP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA