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The map label for this gene is fen

Identifier: 159040632

GI number: 159040632

Start: 54915

End: 55967

Strand: Reverse

Name: fen

Synonym: Cmaq_0040

Alternate gene names: 159040632

Gene position: 55967-54915 (Counterclockwise)

Preceding gene: 159040633

Following gene: 159040631

Centisome position: 2.69

GC content: 45.68

Gene sequence:

>1053_bases
GTGGGTGTTACTGAGCTTGGTAAGCTTATTCCAGATAACTTGAGGAGGAGGGTGAGTCTTGAGCAGCTTAACGGTAAGTT
AATAGCGTTAGATGCGTATAATGCTCTTTATCAATTCCTAGCATCAATAAGGCAACCCGATGGTACACCGCTCATGGATT
CCCAAGGCAGGGTCACCAGTCACTTAAGTGGATTACTCTACAGGACAATTAACCTGCTTGAGTATGGTATTAAACCAGTC
TATGTGTTTGATGGTAAGCCCCCTGAATTGAAGCTTATTGAGATTGAGAAGAGGAGGAGGGTTAGGGAGAAGGCTGTGGA
GGATTGGATTAAGGCTGTTGAGGAGGGTAAGAAGAGTGAGGCTAGGAAGTATGCGCAGAGGGCATTATTCATAACGAGTG
ATATGGTTGATGAGGCTAAAAGACTCCTAGACTCCATGGGTGTACCCTGGGTTCAGGCTCCAAGCGAGGGGGAGGCTCAA
GCGGCGTATATGGCTAGTAAGGGTATCGTGTGGGCTGCCGGCAGCCAGGATTACGACTCATTCTTATTCGGTGCCCCTAG
GCTTGTTAGGAACTTAACTATTTCAGGTAGAAGAAAGTTACCGGGTAGGGATGAGTACGTTGAGGTTACACCGGAGTTAA
TAGAGTTGAATGATGTTCTTAAGGCGCTTAGGTTAAGGGATAGGGGTCAATTAATAGACTTAGCCATACTACTGGGTACT
GATTATAATCCTGAAGGAATACCTGGTATTGGTCCACAGAGGGCTCTTAGGTTGATTCAGGAATATGGTAGCCTAGATAA
ACTCATGAATACCGTGCTTAAGAACGCTCAATTCCCCGTGGACCCCTTTAAAATAAGGGAATTCTTCCTCAACCCACCAG
TGACCCAGGAGGTTAATGTTAAGTTTAAGGAACCTAATGAGGATGAGGTAGTTAGGCTACTTGTGGAGGAGCATGACTTC
AGCCAAGACAGGGTTAAGAATGCATTAGAAAGATTAAGAAAAAGCATGGGTAAGGCTAAGGGATCCACGACCCTCGACTC
ATTCTTCGGTTGA

Upstream 100 bases:

>100_bases
CCAATGCTAGTGGTTGCAGGGGTTTTGCCTTAACTAGAGGGTAATGTGTGGTTGAAGTAATGGGAAGATAAGTTAATACG
CTGGGGCTAGATTAGGCTGT

Downstream 100 bases:

>100_bases
TTAAAATGACCAAGCAGAGAAGGGTAGATTGCGTATACTTGCTTAAACTAGTGAAACTACTTGAGGACCCATTCTCGGGC
TACTACAGTGATGGGTACTT

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGVTELGKLIPDNLRRRVSLEQLNGKLIALDAYNALYQFLASIRQPDGTPLMDSQGRVTSHLSGLLYRTINLLEYGIKPV
YVFDGKPPELKLIEIEKRRRVREKAVEDWIKAVEEGKKSEARKYAQRALFITSDMVDEAKRLLDSMGVPWVQAPSEGEAQ
AAYMASKGIVWAAGSQDYDSFLFGAPRLVRNLTISGRRKLPGRDEYVEVTPELIELNDVLKALRLRDRGQLIDLAILLGT
DYNPEGIPGIGPQRALRLIQEYGSLDKLMNTVLKNAQFPVDPFKIREFFLNPPVTQEVNVKFKEPNEDEVVRLLVEEHDF
SQDRVKNALERLRKSMGKAKGSTTLDSFFG

Sequences:

>Translated_350_residues
MGVTELGKLIPDNLRRRVSLEQLNGKLIALDAYNALYQFLASIRQPDGTPLMDSQGRVTSHLSGLLYRTINLLEYGIKPV
YVFDGKPPELKLIEIEKRRRVREKAVEDWIKAVEEGKKSEARKYAQRALFITSDMVDEAKRLLDSMGVPWVQAPSEGEAQ
AAYMASKGIVWAAGSQDYDSFLFGAPRLVRNLTISGRRKLPGRDEYVEVTPELIELNDVLKALRLRDRGQLIDLAILLGT
DYNPEGIPGIGPQRALRLIQEYGSLDKLMNTVLKNAQFPVDPFKIREFFLNPPVTQEVNVKFKEPNEDEVVRLLVEEHDF
SQDRVKNALERLRKSMGKAKGSTTLDSFFG
>Mature_349_residues
GVTELGKLIPDNLRRRVSLEQLNGKLIALDAYNALYQFLASIRQPDGTPLMDSQGRVTSHLSGLLYRTINLLEYGIKPVY
VFDGKPPELKLIEIEKRRRVREKAVEDWIKAVEEGKKSEARKYAQRALFITSDMVDEAKRLLDSMGVPWVQAPSEGEAQA
AYMASKGIVWAAGSQDYDSFLFGAPRLVRNLTISGRRKLPGRDEYVEVTPELIELNDVLKALRLRDRGQLIDLAILLGTD
YNPEGIPGIGPQRALRLIQEYGSLDKLMNTVLKNAQFPVDPFKIREFFLNPPVTQEVNVKFKEPNEDEVVRLLVEEHDFS
QDRVKNALERLRKSMGKAKGSTTLDSFFG

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=360, Percent_Identity=39.7222222222222, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI194018535, Length=266, Percent_Identity=32.3308270676692, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018531, Length=266, Percent_Identity=32.3308270676692, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI284055276, Length=265, Percent_Identity=26.7924528301887, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI284172361, Length=265, Percent_Identity=26.7924528301887, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI284055278, Length=265, Percent_Identity=26.7924528301887, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51988900, Length=159, Percent_Identity=31.4465408805031, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=358, Percent_Identity=37.9888268156425, Blast_Score=237, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6322736, Length=362, Percent_Identity=38.6740331491713, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324607, Length=274, Percent_Identity=25.1824817518248, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321697, Length=148, Percent_Identity=35.1351351351351, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320469, Length=260, Percent_Identity=24.2307692307692, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17647423, Length=361, Percent_Identity=39.0581717451524, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24658219, Length=266, Percent_Identity=31.5789473684211, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17137168, Length=264, Percent_Identity=26.1363636363636, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI78706850, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78706852, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78706854, Length=138, Percent_Identity=29.7101449275362, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_CALMQ (A8M9L3)

Other databases:

- EMBL:   CP000852
- RefSeq:   YP_001539884.1
- ProteinModelPortal:   A8M9L3
- SMR:   A8M9L3
- GeneID:   5710318
- GenomeReviews:   CP000852_GR
- KEGG:   cma:Cmaq_0040
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- ProtClustDB:   PRK03980
- BioCyc:   CMAQ397948:CMAQ_0040-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 39604; Mature: 39473

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTELGKLIPDNLRRRVSLEQLNGKLIALDAYNALYQFLASIRQPDGTPLMDSQGRVTS
CCHHHHHHHCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
HLSGLLYRTINLLEYGIKPVYVFDGKPPELKLIEIEKRRRVREKAVEDWIKAVEEGKKSE
HHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
ARKYAQRALFITSDMVDEAKRLLDSMGVPWVQAPSEGEAQAAYMASKGIVWAAGSQDYDS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCEEEECCCCCHHH
FLFGAPRLVRNLTISGRRKLPGRDEYVEVTPELIELNDVLKALRLRDRGQLIDLAILLGT
HHHCCHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
DYNPEGIPGIGPQRALRLIQEYGSLDKLMNTVLKNAQFPVDPFKIREFFLNPPVTQEVNV
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEE
KFKEPNEDEVVRLLVEEHDFSQDRVKNALERLRKSMGKAKGSTTLDSFFG
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure 
GVTELGKLIPDNLRRRVSLEQLNGKLIALDAYNALYQFLASIRQPDGTPLMDSQGRVTS
CHHHHHHHCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
HLSGLLYRTINLLEYGIKPVYVFDGKPPELKLIEIEKRRRVREKAVEDWIKAVEEGKKSE
HHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
ARKYAQRALFITSDMVDEAKRLLDSMGVPWVQAPSEGEAQAAYMASKGIVWAAGSQDYDS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCEEEECCCCCHHH
FLFGAPRLVRNLTISGRRKLPGRDEYVEVTPELIELNDVLKALRLRDRGQLIDLAILLGT
HHHCCHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
DYNPEGIPGIGPQRALRLIQEYGSLDKLMNTVLKNAQFPVDPFKIREFFLNPPVTQEVNV
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEE
KFKEPNEDEVVRLLVEEHDFSQDRVKNALERLRKSMGKAKGSTTLDSFFG
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA