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The map label for this gene is thiI [H]

Identifier: 159040606

GI number: 159040606

Start: 26412

End: 27662

Strand: Reverse

Name: thiI [H]

Synonym: Cmaq_0014

Alternate gene names: 159040606

Gene position: 27662-26412 (Counterclockwise)

Preceding gene: 159040607

Following gene: 159040604

Centisome position: 1.33

GC content: 42.85

Gene sequence:

>1251_bases
ATGAACCTTAACCCAATTGTACTCGTTAGGTATGGGGAAATAGGAGTGAAGAGTAATAGGGTTAGGGTTAGGTTGGAGAA
CCTGCTCACTAAGAATATTCAGGAAGCCTTAAGGAGAGGAGGTGTATTAAACTATAGTATTAGTAAGACTAGGGGTAGGA
TTCTGATTAACGTGCCTAAGGAGAATTTAATTAATACAGCCCTAATGGCCGCGAGGGTTTTTGGCGTTGTATCAACATCA
CCAGCCTACTCGCTTAACTTCAGTAGCATTAATGATATAGTTATTGCGGCCTATGAACTATGGCGTGGTAAGGTGAATGG
GAGGAAGTTCGCGGTCAGGGTTAGTAGGACTGGTAATCACCCATTCACATCAATTGACGTGGCTAAGCGTGTTGGTGCAG
TACTCTACCCATTCTCCAATGGTGTTGACTTAGATAACCCGGAGGTGGAGTTGTATGTGGAGATTAGGGATAATTACGCC
TTCCTATTCGATGAGGTAATAGATGGGCCAGGTGGCTTACCTTTAGGTTCACAGGGAGGTAAGGTACTTGCCCTAGTCTC
CGGTGGCTTTGACTCACCTGTGGCTTATTGGTTAATGAGTAGGAGGGGGGCCTTGGTTGATGCATTGTTCTGCAGTCTAG
CCCCACCAGTGGATGTTATTGGGTTAATTAGGGTTATTAGGTATCTTTACGAGAATTGGGTATTTGGGTACGACCCATTA
ATCATGATCGCTGACTGCACTCAATTAGTTAACGCCATGAGGAGTTCAGTTAACAGCCACTTAATGAACACTGTCTTTAA
GAAATTCCTATACAGGTTAGCTGAATCAATAGCTGTGGAGGGTGGTTACATGGGTATAGTTACCGGTGAATCCCTAGGTC
AAGTTAGTAGTCAAACCTTAAGTAACCTGTACTCAGCATCAGCGGGTATTAATGTGCCTATATTCAGGCCCTTAATAGGT
ATGGATAAGGATGATATAATTAAGTTAGCTAAGAGGATAGGTACGTATGAGGAATCAGTGAAAATGATTGAACCCTGCTC
AGTGTTCTCTAGGAAGCCGAGGACTAGATCAACACCAAGTAGGCTTGACGAGGAATTAAGCAGGGTAATTAACCTATTAG
GCGTAATTAAGGCAAGTATTATTAAGGTAAAGGCCAGTGAATTAAGCAGTATTAATGAAAGGTCACTGTTAAGTAACTGG
AGAATCAACATTATGGGCACTGATGCAGGTGGTTTAGGTTCATGTAAGTGA

Upstream 100 bases:

>100_bases
GGCTTGTGCTCAAGTACATTAGAGGTAAGTCGAATTTAACCAACAGTATAAGTCAGGTTCATGGATAAGAAATATTAATA
CCACAGGAGCCTTGGGTCTA

Downstream 100 bases:

>100_bases
AGCATACTGAATAAGGTGTTTAACCCAAGGTTTAATTAACTACGTAGCTTACAATGAAGTTGAGTAACTGTGATATCTTA
TATCTACTAAGATACCAGAG

Product: thiamine biosynthesis/tRNA modification protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNLNPIVLVRYGEIGVKSNRVRVRLENLLTKNIQEALRRGGVLNYSISKTRGRILINVPKENLINTALMAARVFGVVSTS
PAYSLNFSSINDIVIAAYELWRGKVNGRKFAVRVSRTGNHPFTSIDVAKRVGAVLYPFSNGVDLDNPEVELYVEIRDNYA
FLFDEVIDGPGGLPLGSQGGKVLALVSGGFDSPVAYWLMSRRGALVDALFCSLAPPVDVIGLIRVIRYLYENWVFGYDPL
IMIADCTQLVNAMRSSVNSHLMNTVFKKFLYRLAESIAVEGGYMGIVTGESLGQVSSQTLSNLYSASAGINVPIFRPLIG
MDKDDIIKLAKRIGTYEESVKMIEPCSVFSRKPRTRSTPSRLDEELSRVINLLGVIKASIIKVKASELSSINERSLLSNW
RINIMGTDAGGLGSCK

Sequences:

>Translated_416_residues
MNLNPIVLVRYGEIGVKSNRVRVRLENLLTKNIQEALRRGGVLNYSISKTRGRILINVPKENLINTALMAARVFGVVSTS
PAYSLNFSSINDIVIAAYELWRGKVNGRKFAVRVSRTGNHPFTSIDVAKRVGAVLYPFSNGVDLDNPEVELYVEIRDNYA
FLFDEVIDGPGGLPLGSQGGKVLALVSGGFDSPVAYWLMSRRGALVDALFCSLAPPVDVIGLIRVIRYLYENWVFGYDPL
IMIADCTQLVNAMRSSVNSHLMNTVFKKFLYRLAESIAVEGGYMGIVTGESLGQVSSQTLSNLYSASAGINVPIFRPLIG
MDKDDIIKLAKRIGTYEESVKMIEPCSVFSRKPRTRSTPSRLDEELSRVINLLGVIKASIIKVKASELSSINERSLLSNW
RINIMGTDAGGLGSCK
>Mature_416_residues
MNLNPIVLVRYGEIGVKSNRVRVRLENLLTKNIQEALRRGGVLNYSISKTRGRILINVPKENLINTALMAARVFGVVSTS
PAYSLNFSSINDIVIAAYELWRGKVNGRKFAVRVSRTGNHPFTSIDVAKRVGAVLYPFSNGVDLDNPEVELYVEIRDNYA
FLFDEVIDGPGGLPLGSQGGKVLALVSGGFDSPVAYWLMSRRGALVDALFCSLAPPVDVIGLIRVIRYLYENWVFGYDPL
IMIADCTQLVNAMRSSVNSHLMNTVFKKFLYRLAESIAVEGGYMGIVTGESLGQVSSQTLSNLYSASAGINVPIFRPLIG
MDKDDIIKLAKRIGTYEESVKMIEPCSVFSRKPRTRSTPSRLDEELSRVINLLGVIKASIIKVKASELSSINERSLLSNW
RINIMGTDAGGLGSCK

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=360, Percent_Identity=33.6111111111111, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF00581 Rhodanese; PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45736; Mature: 45736

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNPIVLVRYGEIGVKSNRVRVRLENLLTKNIQEALRRGGVLNYSISKTRGRILINVPK
CCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCH
ENLINTALMAARVFGVVSTSPAYSLNFSSINDIVIAAYELWRGKVNGRKFAVRVSRTGNH
HHHHHHHHHHHHHHHHEECCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCC
PFTSIDVAKRVGAVLYPFSNGVDLDNPEVELYVEIRDNYAFLFDEVIDGPGGLPLGSQGG
CCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCC
KVLALVSGGFDSPVAYWLMSRRGALVDALFCSLAPPVDVIGLIRVIRYLYENWVFGYDPL
EEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
IMIADCTQLVNAMRSSVNSHLMNTVFKKFLYRLAESIAVEGGYMGIVTGESLGQVSSQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHH
SNLYSASAGINVPIFRPLIGMDKDDIIKLAKRIGTYEESVKMIEPCSVFSRKPRTRSTPS
HHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCCHH
RLDEELSRVINLLGVIKASIIKVKASELSSINERSLLSNWRINIMGTDAGGLGSCK
HHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHHHHHCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNLNPIVLVRYGEIGVKSNRVRVRLENLLTKNIQEALRRGGVLNYSISKTRGRILINVPK
CCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCH
ENLINTALMAARVFGVVSTSPAYSLNFSSINDIVIAAYELWRGKVNGRKFAVRVSRTGNH
HHHHHHHHHHHHHHHHEECCCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCC
PFTSIDVAKRVGAVLYPFSNGVDLDNPEVELYVEIRDNYAFLFDEVIDGPGGLPLGSQGG
CCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCC
KVLALVSGGFDSPVAYWLMSRRGALVDALFCSLAPPVDVIGLIRVIRYLYENWVFGYDPL
EEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCH
IMIADCTQLVNAMRSSVNSHLMNTVFKKFLYRLAESIAVEGGYMGIVTGESLGQVSSQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHH
SNLYSASAGINVPIFRPLIGMDKDDIIKLAKRIGTYEESVKMIEPCSVFSRKPRTRSTPS
HHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCCHH
RLDEELSRVINLLGVIKASIIKVKASELSSINERSLLSNWRINIMGTDAGGLGSCK
HHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCHHHHHHCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]