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The map label for this gene is proP [H]

Identifier: 15899850

GI number: 15899850

Start: 2897100

End: 2898353

Strand: Direct

Name: proP [H]

Synonym: SSO3147

Alternate gene names: 15899850

Gene position: 2897100-2898353 (Clockwise)

Preceding gene: 15899847

Following gene: 15899855

Centisome position: 96.82

GC content: 37.4

Gene sequence:

>1254_bases
ATGTTAAACGAGATGAGCTCACTATCAAGCAGGGAAATTAAGTTAATATTCCTAAATTTCATGAGTTATACATTTCTAGT
ATATAATTATTCGATATTGTTAGTATTTAACTCACCATATATCTCTCAAACGTTATTTAAAGGTTCGTACGTACTCTCAC
TATTAGGGGTTTATGGCTTACTATTAATAGACGTAGTTATGAGAGTGCCTGGGGCTTACGTATTGGGACCAATAAGCGAT
AGGTATGGTAGGAAGCTAGTTACCAGAATATCCAGTTTAGGCTCAGCTTTGCCTTTAGTTGCAGTAGCCCTAATACCAGA
TCCCTCTCCCTTGTTATTAATAATACTATATGTGATACAAGGTTTCTTTACGGGAGGATTATCAGCTGGGATAACTGTAG
TAGGTGTCGAGGATTTGCCGGAAAGACATAGGGGTTGGTTTGGAGGATCTGGATTTGCTGTTGGTGGTTCAGCGTACCTT
CTAGCGTCAATAGTGTTTTTCATAATAATTACCGTAATAGGTAGTTATAACTATATTGAAATAGGATGGAGAATAATGTT
CTTGACCTCTCTTCTTATCTTGCCCTTTGGGTTCTTAATGCCTGAGTCCTTAAGGTTTAGGAGAAATAAGGAGAGGGTAA
AATCACCTGCTAAGCTGTTAATTTCGTCATACAAGAGACAATTTATATTGTCATCAACGTTAACGGCTCTTTGGGCTTCG
ATGAATGCGTTAGTTAACGTCATGTTACCTAATTTTCTTTACGCCGTTAATCACTTATCAAAAGTTGAAATAGGATACAT
GTCCTTGATATACAGTGTAATAGCTATCATTTCGGCATTTATGGGAGGTGAATTGAGTGAAAAAATTGGCAGAAAGAAGG
TATCAATGATAGGTGGAATTTTTGGAATTTTGCTCTCACCAGTTTTCATACTAATGTCCCTATCTTCAAGGTCGTTAATT
CCCATTTTTGTTTCAATTCTGGGCTTCGTATCTGTATTTGGAGGTGGTGGGATAATGAGCTACGTAAATGAGAATTTCCC
AACCAAAATTAGAAGCACTGGGGTATCCTTAAGCTGGAATATAGGGTTCTTAGTTGGAAATTTCGTCCCATTACTTCTCA
TAACTGTCCTTTACCTTACTTCAATAACATTATTCCCACTCGTGGAAATGGTAACTATGATCATTCTTGGCGCATTAATA
ATTTCGGTAAGCGTTATCTCGCATGAGACAAAGGGCAATATAGAGAAGGAGTAG

Upstream 100 bases:

>100_bases
AATCGCTAAATACAGTATTTCAAATGGTGAGTACACGGGAATTTTTAATAGACTATATTGTCTGAGGGAAGACCGATATT
ATTTTTTAGCTAAATGTTAA

Downstream 100 bases:

>100_bases
TTTCTTCCAATCCTCCCTAAATTACACCTCAATTCAATTCCTATAATGATGTCTAACAGCAGCGAACTACCCTCACCGGC
AGACCCAAAATGACCCCCTT

Product: permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGLLLIDVVMRVPGAYVLGPISD
RYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQGFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYL
LASIVFFIIITVIGSYNYIEIGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWAS
MNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGIFGILLSPVFILMSLSSRSLI
PIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWNIGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALI
ISVSVISHETKGNIEKE

Sequences:

>Translated_417_residues
MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGLLLIDVVMRVPGAYVLGPISD
RYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQGFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYL
LASIVFFIIITVIGSYNYIEIGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWAS
MNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGIFGILLSPVFILMSLSSRSLI
PIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWNIGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALI
ISVSVISHETKGNIEKE
>Mature_417_residues
MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGLLLIDVVMRVPGAYVLGPISD
RYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQGFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYL
LASIVFFIIITVIGSYNYIEIGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWAS
MNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGIFGILLSPVFILMSLSSRSLI
PIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWNIGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALI
ISVSVISHETKGNIEKE

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=338, Percent_Identity=26.0355029585799, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788292, Length=334, Percent_Identity=27.2455089820359, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45783; Mature: 45783

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGL
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLIDVVMRVPGAYVLGPISDRYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQ
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHH
GFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYLLASIVFFIIITVIGSYNYIE
HHHHCCHHCCEEEEEHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEH
IGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FGILLSPVFILMSLSSRSLIPIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCEEEEE
IGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALIISVSVISHETKGNIEKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGL
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLIDVVMRVPGAYVLGPISDRYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQ
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHH
GFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYLLASIVFFIIITVIGSYNYIE
HHHHCCHHCCEEEEEHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEH
IGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FGILLSPVFILMSLSSRSLIPIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWN
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCEEEEE
IGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALIISVSVISHETKGNIEKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA