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The map label for this gene is nikA [H]

Identifier: 15899749

GI number: 15899749

Start: 2794177

End: 2796309

Strand: Reverse

Name: nikA [H]

Synonym: SSO3043

Alternate gene names: 15899749

Gene position: 2796309-2794177 (Counterclockwise)

Preceding gene: 15899750

Following gene: 15899746

Centisome position: 93.45

GC content: 38.82

Gene sequence:

>2133_bases
ATGAAAAGGTATAAGATAATTTCTACAATAATTACAGTATTAATGGTAATAAGCATAGGGATATTTGCAATGCCAATACT
ATCACAGTCAACATCAGTACAACCAGAAGGAAGCATGGTAATTATGCCTAGTCCGGGTATAGTATGGCAAGATAACTTTA
ATCCATGGAACGCTCCAGCCTTACCCGGAGGTCTAATTTGGCTTATTTACGAACCATTAGCCCTAGTTAATTCGCTAAGT
GGTCAAATTATTCCATGGTTAGCCACCAATTGGACATTTACTACCGGTTATGTTAACTTGCCAAATGGTACTAAGGTACA
AACATTGGCATTAATATTATACTTAAGGCATGACGTATACTTCACTGACGGCACACCATTTAACGCTACAGCAGTATGGT
ACACATTAGCGTTAGAACAAGCATACCCACAATTGGGATATTCAGCTAATCAAATAGCTAACATGACAATAATAAACCCA
TATGAGCTAGAAATAGTATTTAAACCTGGAGTAACGCCAATGGTATTGTATACTATTTTGGAGCAATGGCCTGTGGACCC
AGCACAATGGGGTAAAATATTCCCAGTAGAGCAATTACCAAATGGAACATATGTAGGACTAAACAAGACTGGAAATCCAT
TCACATACCCTAATACAAATCCAATAGGTACTGGACCTTATATGCTATATAGTTTCAGCCCGCAAGAAATAGTACTAGTG
GCCAACCCACACTATTGGATGCCGGGAGAACCCAGAATAAAATACTTACTCTATCCATCATATCCTAGTAATGTTCAAGC
AGATACTGCATTAAATAATGGGCAAATAACTTGGGCTGGACTATTCGAACCAAATATACAACAGCAATTTGTGGCAAAAG
ATCCTCTACACTATCATTACTATTTTGCTCCTGGATTTCCAACAATGTTGACACTCAATAACCTAAAATGGCCTTTATCC
GATCCAGTTTTAAGACAAGCAATAAGCCTTGCGATCAATAGAACAGCAATATACTATTTGGGCGAATATGGATACGAACC
GCCAGCAACTACGCCTCTTCCATTACCTCCACAACAGCTACAATATATAAATTCGTCGGTGTTGCAACTTGCTGAGCAAT
TCGCTCCACCTCAAGGTAATGTTACTGCTGCATTACAGTTGCTTGAATCCCACGGCTATAAATTAGTTAATGGCCAACTA
ATTACACCAAATGGTACTGCAGTACCAGCAATGACCATCATTACGGTAGCCGGATGGACTGATTGGGACGCCGATTTAAC
TTTAATTGCACAAGATTTGAAGCAAATAGGCATAACTGTGGAAGTACAAACACCACCGTTTACAACATGGTATAATTACA
TGGAAACTGGCAATTACTGGATGGGACTAATGTGGGATTTAATTAGCGGTCCTACTCCTATACAACCACTTTCTGGGTAT
TTGTATGGTTATTGGAATTCCCCCGGCAACATAACACCGATAGGAAATAGCACATATTATAACATAGAAAGGTTTAATTT
ATCAATTATCCATCCAACGTTCGAACAACTTGTGAACTGGGCAAATGGTAACTTCAGCATAAACGATAATGCCTATTATA
GTATAGTAAATAAGCTAGCTGCTCTATGGATAAAATATATGCCTTCCATTGCTCTAGTATACGGCGCAGAGTGGTATGAG
TATGTTAACAATACTGTTACTGGTTGGCCAACTCAGCAAAACTATTATTGGCCGGCTCCCCCATGGACCAGTCTTCCCTC
AACTCCTTTACCTGTTGTTTTAGCCTTACATTTGGTTAATCAGTCTGTTCCTGAGCCTTGGTGGTATTATACTTCACAAG
TTCCTTCAAGTTGGTATACTTCCAATGACCCATTCGTGTATCAAACAACCACAACTACTACAACTAGCACTACCACAACT
ACATCAACTACTACAACTAGCACTACCACAAGTACTGTTACTACCACAAGTGTTTCAACTACCACAAGTGTATCCACATC
CGTAAGTACCACCACAGCTACTGTTACTACTACTGTAACTTCATCATCCAATACTACACTATATGCCATAATAGCAGTAG
TAGTTATAATCATAGTAATAATAGGAGTAATACTAGGATTAAGAAGGAGATAA

Upstream 100 bases:

>100_bases
TATCACTAATTAGGTTGCTAGAAAATTGTGATTTTAATTACTGAGAAAAGTTTAAATATAGCCTAATATACCATTATAAA
CAGATTAATGGTGTATAGGT

Downstream 100 bases:

>100_bases
AATAAAATAATAATGAAAAGAAGAAATTTTTCTTTTTTGCCTTTTTATTCCCATACTATTTTGACTTTTATTTCCCCCGG
AAGCTTTTCCCTAAGACTAC

Product: ABC transporter, binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MKRYKIISTIITVLMVISIGIFAMPILSQSTSVQPEGSMVIMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQIIPWLATNWTFTTGYVNLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEIVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYVGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQLQYINSSVLQLAEQFAPPQGNVTAALQLLESHGYKLVNGQL
ITPNGTAVPAMTIITVAGWTDWDADLTLIAQDLKQIGITVEVQTPPFTTWYNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYYNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTSTTTT
TSTTTTSTTTSTVTTTSVSTTTSVSTSVSTTTATVTTTVTSSSNTTLYAIIAVVVIIIVIIGVILGLRRR

Sequences:

>Translated_710_residues
MKRYKIISTIITVLMVISIGIFAMPILSQSTSVQPEGSMVIMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQIIPWLATNWTFTTGYVNLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEIVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYVGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQLQYINSSVLQLAEQFAPPQGNVTAALQLLESHGYKLVNGQL
ITPNGTAVPAMTIITVAGWTDWDADLTLIAQDLKQIGITVEVQTPPFTTWYNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYYNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTSTTTT
TSTTTTSTTTSTVTTTSVSTTTSVSTSVSTTTATVTTTVTSSSNTTLYAIIAVVVIIIVIIGVILGLRRR
>Mature_710_residues
MKRYKIISTIITVLMVISIGIFAMPILSQSTSVQPEGSMVIMPSPGIVWQDNFNPWNAPALPGGLIWLIYEPLALVNSLS
GQIIPWLATNWTFTTGYVNLPNGTKVQTLALILYLRHDVYFTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINP
YELEIVFKPGVTPMVLYTILEQWPVDPAQWGKIFPVEQLPNGTYVGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHYYFAPGFPTMLTLNNLKWPLS
DPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQLQYINSSVLQLAEQFAPPQGNVTAALQLLESHGYKLVNGQL
ITPNGTAVPAMTIITVAGWTDWDADLTLIAQDLKQIGITVEVQTPPFTTWYNYMETGNYWMGLMWDLISGPTPIQPLSGY
LYGYWNSPGNITPIGNSTYYNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLAALWIKYMPSIALVYGAEWYE
YVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVNQSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTSTTTT
TSTTTTSTTTSTVTTTSVSTTTSVSTSVSTTTATVTTTVTSSSNTTLYAIIAVVVIIIVIIGVILGLRRR

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=406, Percent_Identity=27.3399014778325, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787052, Length=340, Percent_Identity=25.2941176470588, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 79134; Mature: 79134

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYKIISTIITVLMVISIGIFAMPILSQSTSVQPEGSMVIMPSPGIVWQDNFNPWNAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCC
LPGGLIWLIYEPLALVNSLSGQIIPWLATNWTFTTGYVNLPNGTKVQTLALILYLRHDVY
CCCCEEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCEEE
FTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINPYELEIVFKPGVTPMVLYTIL
EECCCCCCCCEEEEEEHHHHHHHHCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHH
EQWPVDPAQWGKIFPVEQLPNGTYVGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
HHCCCCHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHY
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEE
YFAPGFPTMLTLNNLKWPLSDPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQL
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHH
QYINSSVLQLAEQFAPPQGNVTAALQLLESHGYKLVNGQLITPNGTAVPAMTIITVAGWT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEEEEECCC
DWDADLTLIAQDLKQIGITVEVQTPPFTTWYNYMETGNYWMGLMWDLISGPTPIQPLSGY
CCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
LYGYWNSPGNITPIGNSTYYNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLA
EEEEECCCCCEEECCCCEEEEEEEEEEEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ALWIKYMPSIALVYGAEWYEYVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVN
HHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
QSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTSTTTTTSTTTTSTTTSTVTTTSVST
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEECEECCCEEECCEEECCCEEEEEEEEEEEC
TTSVSTSVSTTTATVTTTVTSSSNTTLYAIIAVVVIIIVIIGVILGLRRR
CCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRYKIISTIITVLMVISIGIFAMPILSQSTSVQPEGSMVIMPSPGIVWQDNFNPWNAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCC
LPGGLIWLIYEPLALVNSLSGQIIPWLATNWTFTTGYVNLPNGTKVQTLALILYLRHDVY
CCCCEEEEEHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCEEE
FTDGTPFNATAVWYTLALEQAYPQLGYSANQIANMTIINPYELEIVFKPGVTPMVLYTIL
EECCCCCCCCEEEEEEHHHHHHHHCCCCHHHEEEEEEECCEEEEEEECCCCCHHHHHHHH
EQWPVDPAQWGKIFPVEQLPNGTYVGLNKTGNPFTYPNTNPIGTGPYMLYSFSPQEIVLV
HHCCCCHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
ANPHYWMPGEPRIKYLLYPSYPSNVQADTALNNGQITWAGLFEPNIQQQFVAKDPLHYHY
ECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCEEEE
YFAPGFPTMLTLNNLKWPLSDPVLRQAISLAINRTAIYYLGEYGYEPPATTPLPLPPQQL
EECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHH
QYINSSVLQLAEQFAPPQGNVTAALQLLESHGYKLVNGQLITPNGTAVPAMTIITVAGWT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCEEECCCCCCCCEEEEEEEECCC
DWDADLTLIAQDLKQIGITVEVQTPPFTTWYNYMETGNYWMGLMWDLISGPTPIQPLSGY
CCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
LYGYWNSPGNITPIGNSTYYNIERFNLSIIHPTFEQLVNWANGNFSINDNAYYSIVNKLA
EEEEECCCCCEEECCCCEEEEEEEEEEEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ALWIKYMPSIALVYGAEWYEYVNNTVTGWPTQQNYYWPAPPWTSLPSTPLPVVLALHLVN
HHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
QSVPEPWWYYTSQVPSSWYTSNDPFVYQTTTTTTTSTTTTTSTTTTSTTTSTVTTTSVST
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEECEECCCEEECCEEECCCEEEEEEEEEEEC
TTSVSTSVSTTTATVTTTVTSSSNTTLYAIIAVVVIIIVIIGVILGLRRR
CCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]