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The map label for this gene is ilvB-5 [H]

Identifier: 15899670

GI number: 15899670

Start: 2704945

End: 2706603

Strand: Reverse

Name: ilvB-5 [H]

Synonym: SSO2962

Alternate gene names: 15899670

Gene position: 2706603-2704945 (Counterclockwise)

Preceding gene: 15899671

Following gene: 15899669

Centisome position: 90.45

GC content: 36.89

Gene sequence:

>1659_bases
ATGGCAAAAACAGTAGCAGATATAATAGTTGAGGCATTAGTTAAGGCTGGCATTAAAAGAATATACGGAATACCTGGAGA
TTCAATTGATCCATTGATGGACGCCATAAGAAGAAATAAAGAAATTAAATATATACAAGTTAGGCATGAAGAGGGAGCAG
CATTTGCTGCATCAGTCGAATCTAAACTCACATCTAACGTTACTGCTTGTATGGGTACGTCTGGTCCTGGCTCAATCCAC
CTATTAAATGGGTTATATGACGCTAAAATGGACCACGCATCGGTAGTAGCTCTTACTGGGCAGATTGAAAGCGATATGAT
TGGTCATGACTACTTTCAAGAAGTAAATATGATAAAACTATTTGATGACGTTGCGGTATTTAATCAAATAATAATAAATC
CGAAAAATGCCGGCTATTTAATTTGGAGAGCGGTATATGAGGCTAAAAGTAAGAAGGGTGTAGCTCATCTTAACCTACCC
GTGGATATTTTAAGAATGGAAGGGGAGGAATACGAAGGCGAATTCTATTATCCTAAAATAAGCTATGATTATCCAGACTT
AGACAAGGCTATAGAGCTCATAGATAAAAGCAAATCTCCAGTGATAATGATTGGTGGAGGTGCAAGAGATAAGTCAAAGA
TAGTAAACGATTTTAGTGAGAAGATTGGGGCGCCTATAATTTACGCTCTTAACGGTAAGGGAATTTTATCTGATTACGAT
GATAAAGTTATGGGTGGGATAGGTTTACTAGGGACAAAACCATCAGTAGACGCATTAAATAAGACTGACTTACTTATACT
TTTAGGAACGTCTTTTCCTTACGTGAATTTCTTACCTAAGGATGTGGATGTTATTCAAGTTGATAATAATCCAGCAAATA
TAGGCAAAAGAATAAAAGTATCTTTACCTATTGTCAGTGATGTAGGAGAATTCCTATTAAAAATAACGCCGAAAATTAAA
GAAAAGGATGACAAGTTTTATCTAAAAATGAAAGATTCAAAGGAAAAATGGTTTAAGGAGCTAAGTAAGGCAGAAAATGA
TAGCTCTAAGCCAATGAAGCCTCAAAGAGTGGCTTTTGAATTATCAAAAGTTTTCACCGAAGGAGTAGTCGTAGTGGATA
CTGGAAATGTGACCATGTGGGGGGCTAGGAATTTTAGAGCATCTGGCAAGCAAAAATTTGTGTTTTCAGCATGGTTAGGA
TCAATGGGTATAGGAATACCCGGTGCAGTAGGTGCCTCATTTGTATCTGATGAGGTTTTAGCGTTAGTAGGTGATGGAGG
CTTTGCTATGACGATGATGGAGCTTATAACTGCAAAGAAATACTCAAGGCCAATAAAGGTAGTAATATTTAACAATTCTA
AATTAGGTATGATTAAGTTTGAACAAGAGGTTATGGGATATCCAGAGTGGGGTGTAGAATTATACAATCCAGATTTTTCT
AGATTGGCTGAATCCATCGGAGTAAGAAGTGTAACCGTCGAGGATCCAAATGAACTCTCATCAGCTATTAAGGAGATGAA
GGAAAGCGAAGAACCCTTTGTTCTTAATACGGTTGTTGATCCTAATGAAAGACCAATGCCACCTAAACTTACTTTTACTC
AAGCTAAAAATTACATATTATCTGTATTTAGGGAAAAATTGGAGCCCAGTGAAGAGTGA

Upstream 100 bases:

>100_bases
AATTTACTTTATATTATGAAAAATTTAGTTTATTGATTATCATTCTCATTTTCTAGGTTATAAATATAAGATTTTTAGCT
ATATTATAATAAGATAAGGT

Downstream 100 bases:

>100_bases
TAAGTAGTGAGAGTTGTAATAGTTGGCGGAGGATTTGCTGGTATCGCAGCTAAAAGTAAATATCCTAATGCAATTTTAAT
AGATAGAAGCGAATATTTTT

Product: pyruvate dehydrogenase

Products: acetate; CO2; ferrocytochrome b1

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MAKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVESKLTSNVTACMGTSGPGSIH
LLNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKLFDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLP
VDILRMEGEEYEGEFYYPKISYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGILSDYD
DKVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGKRIKVSLPIVSDVGEFLLKITPKIK
EKDDKFYLKMKDSKEKWFKELSKAENDSSKPMKPQRVAFELSKVFTEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLG
SMGIGIPGAVGASFVSDEVLALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEWGVELYNPDFS
RLAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPKLTFTQAKNYILSVFREKLEPSEE

Sequences:

>Translated_552_residues
MAKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVESKLTSNVTACMGTSGPGSIH
LLNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKLFDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLP
VDILRMEGEEYEGEFYYPKISYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGILSDYD
DKVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGKRIKVSLPIVSDVGEFLLKITPKIK
EKDDKFYLKMKDSKEKWFKELSKAENDSSKPMKPQRVAFELSKVFTEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLG
SMGIGIPGAVGASFVSDEVLALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEWGVELYNPDFS
RLAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPKLTFTQAKNYILSVFREKLEPSEE
>Mature_551_residues
AKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVESKLTSNVTACMGTSGPGSIHL
LNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKLFDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLPV
DILRMEGEEYEGEFYYPKISYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGILSDYDD
KVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGKRIKVSLPIVSDVGEFLLKITPKIKE
KDDKFYLKMKDSKEKWFKELSKAENDSSKPMKPQRVAFELSKVFTEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLGS
MGIGIPGAVGASFVSDEVLALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEWGVELYNPDFSR
LAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPKLTFTQAKNYILSVFREKLEPSEE

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=24.2857142857143, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=33.9928057553957, Blast_Score=324, Evalue=8e-90,
Organism=Escherichia coli, GI87081685, Length=555, Percent_Identity=27.5675675675676, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1790104, Length=543, Percent_Identity=26.3351749539595, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=26.3063063063063, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=25.8652094717668, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=25.8347978910369, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=25.8347978910369, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=589, Percent_Identity=19.8641765704584, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=26.4285714285714, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=23.7076648841355, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=24.2530755711775, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2

Molecular weight: Translated: 61094; Mature: 60963

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVE
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHH
SKLTSNVTACMGTSGPGSIHLLNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKL
HHHHCCCCEEECCCCCCCEEEECCHHHCCCCCCEEEEEECCCCCCCCCHHHHHHCCHHHH
FDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLPVDILRMEGEEYEGEFYYPKI
HHHHHHHHHEEECCCCCCEEEEHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCEEEEEE
SYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGILSDYD
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
DKVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGKRIKV
CHHHCCEEECCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEECCCCCCCCCEEEE
SLPIVSDVGEFLLKITPKIKEKDDKFYLKMKDSKEKWFKELSKAENDSSKPMKPQRVAFE
EECCHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHH
LSKVFTEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLGSMGIGIPGAVGASFVSDEVL
HHHHHHCCEEEEECCCEEEEECCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHCCCEE
ALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEWGVELYNPDFS
EEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCEEECCCHH
RLAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPKLTFTQAKNYIL
HHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHH
SVFREKLEPSEE
HHHHHHCCCCCC
>Mature Secondary Structure 
AKTVADIIVEALVKAGIKRIYGIPGDSIDPLMDAIRRNKEIKYIQVRHEEGAAFAASVE
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHH
SKLTSNVTACMGTSGPGSIHLLNGLYDAKMDHASVVALTGQIESDMIGHDYFQEVNMIKL
HHHHCCCCEEECCCCCCCEEEECCHHHCCCCCCEEEEEECCCCCCCCCHHHHHHCCHHHH
FDDVAVFNQIIINPKNAGYLIWRAVYEAKSKKGVAHLNLPVDILRMEGEEYEGEFYYPKI
HHHHHHHHHEEECCCCCCEEEEHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCEEEEEE
SYDYPDLDKAIELIDKSKSPVIMIGGGARDKSKIVNDFSEKIGAPIIYALNGKGILSDYD
CCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
DKVMGGIGLLGTKPSVDALNKTDLLILLGTSFPYVNFLPKDVDVIQVDNNPANIGKRIKV
CHHHCCEEECCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEEECCCCCCCCCEEEE
SLPIVSDVGEFLLKITPKIKEKDDKFYLKMKDSKEKWFKELSKAENDSSKPMKPQRVAFE
EECCHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHH
LSKVFTEGVVVVDTGNVTMWGARNFRASGKQKFVFSAWLGSMGIGIPGAVGASFVSDEVL
HHHHHHCCEEEEECCCEEEEECCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHCCCEE
ALVGDGGFAMTMMELITAKKYSRPIKVVIFNNSKLGMIKFEQEVMGYPEWGVELYNPDFS
EEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCEEECCCHH
RLAESIGVRSVTVEDPNELSSAIKEMKESEEPFVLNTVVDPNERPMPPKLTFTQAKNYIL
HHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHH
SVFREKLEPSEE
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: pyruvate; ferricytochrome b1; H2O

Specific reaction: pyruvate + ferricytochrome b1 + H2O = acetate + CO2 + ferrocytochrome b1

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]