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The map label for this gene is proP [H]

Identifier: 15899647

GI number: 15899647

Start: 2681354

End: 2682670

Strand: Reverse

Name: proP [H]

Synonym: SSO2938

Alternate gene names: 15899647

Gene position: 2682670-2681354 (Counterclockwise)

Preceding gene: 15899648

Following gene: 15899646

Centisome position: 89.65

GC content: 35.84

Gene sequence:

>1317_bases
ATGGTCTCAGAGAAAACTAAGGCTATAATCGCCTCAAGTTTCGGAATGGCACTAGAATGGTATGATTTCTTTATTTATAC
ATTCGTGGCGACTGTTGTTTCAAAATTATTCTTTCCGTCATCTAATCCAATAGCTTCACTGTTAGCCTTCTATTTAACCT
TCTTTATAGGGTTTTTAGGTAGACCATTAGGTGGTATAGTATTTGGATATTTAGGAGACAAAGTAGGAAGAAAGTATTCA
TTAGTCTTTACGATTTTACTTACTGGACTTAGCGTATTTTTTGTTGGACTCTTACCAACATATTATCAAATAGGTCTATT
AGCGCCAATCTTGCTCGCCATACTAAGGTTCCTTACTGGTGTGGCCTTAGGCGGAGAGTGGGGTGGTGCGTTTTCATTAA
CATCTGAATACGTAAATCCAGGTCGGAGGGGGTTATATTCAGGTATTCTTCAAGCTACAGTATCTATTGCCAACTTGCTA
GCGACTGGTATAATATTTGTTATAATTATAATAATGGGGCAAACTGGCTTCGATAACTTTGGATGGAGAATATTGTTCTA
TTCTGGACTACTCATTGCAATAGTGGGATTACTAATAAGGCTAAGAATAGAAGATTCACCAGTATTTAGGAAGTTGAGGG
AAGATCGAAAAATATTGAAAAATCCAATCTCTGAAGCGTTTAGAAAATACTGGAAGTTAATACTCGCAAACTTATTTATA
GTGGGAATCATTAATGGGGCTTGGTATTATACAAATTTCGCATATTCTGTAGGTTACGCTACAACTATTGCCCAACGCTT
TAATATGCCTCATGTCTCTCTTCAAACAATAGACTTTGCTATATTTTTAGTATCGGCAATAGGCATCTTTGCCTCAATAT
TTTTTGGATATCTGTCCGACATTATAGGAAGAAGGACGCAAATAATAATAAATTCCATAATGGGAATAGGACTGGCGTAT
CCTTACTATTATCTATTACTTCAAGGTAATGAATCAGCAGTAATCGGTGCTATATTTCTTGGTGGACTATTAATATACTA
CTTTGCAGGTGCAATAACGCCTGCATTTCTTGTTGAATCGTTTCCACCGATGGTTAGATATACAGCGATATCATTTTCGT
ATCAGGTAGGAGTTGGATTTATAGGAGGTCTTACACCATTTATCTTGACCTATTTAGTTAGTGCAACTAGTAACATATTC
TCTCCGGTTTACTTTACAATACTCACTGGTATAGTAGTTCTGATACTTGCATTACTTCTTAAAGAGACTAAAGGAAGAAT
TTATGAAGGAGAAGAAATACTGAAGCAGGAATATTAA

Upstream 100 bases:

>100_bases
GGTGAGAGCGGAGTAGTTCACATAAGTATGTTGTGCTAGATATGTACTCTATCCCTTTTAAATATATATATATTGAGGTG
CTACTTATAATTGTGGTAAA

Downstream 100 bases:

>100_bases
GGCATAAGTAAATATATCTTTTTTATTATATTACTATAAGCATTATCAAGTTAATCATTTATTCATATTTTTCTGCAATA
AGGTACATTTGATAATTCTT

Product: permease (proline/betaine)

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MVSEKTKAIIASSFGMALEWYDFFIYTFVATVVSKLFFPSSNPIASLLAFYLTFFIGFLGRPLGGIVFGYLGDKVGRKYS
LVFTILLTGLSVFFVGLLPTYYQIGLLAPILLAILRFLTGVALGGEWGGAFSLTSEYVNPGRRGLYSGILQATVSIANLL
ATGIIFVIIIIMGQTGFDNFGWRILFYSGLLIAIVGLLIRLRIEDSPVFRKLREDRKILKNPISEAFRKYWKLILANLFI
VGIINGAWYYTNFAYSVGYATTIAQRFNMPHVSLQTIDFAIFLVSAIGIFASIFFGYLSDIIGRRTQIIINSIMGIGLAY
PYYYLLLQGNESAVIGAIFLGGLLIYYFAGAITPAFLVESFPPMVRYTAISFSYQVGVGFIGGLTPFILTYLVSATSNIF
SPVYFTILTGIVVLILALLLKETKGRIYEGEEILKQEY

Sequences:

>Translated_438_residues
MVSEKTKAIIASSFGMALEWYDFFIYTFVATVVSKLFFPSSNPIASLLAFYLTFFIGFLGRPLGGIVFGYLGDKVGRKYS
LVFTILLTGLSVFFVGLLPTYYQIGLLAPILLAILRFLTGVALGGEWGGAFSLTSEYVNPGRRGLYSGILQATVSIANLL
ATGIIFVIIIIMGQTGFDNFGWRILFYSGLLIAIVGLLIRLRIEDSPVFRKLREDRKILKNPISEAFRKYWKLILANLFI
VGIINGAWYYTNFAYSVGYATTIAQRFNMPHVSLQTIDFAIFLVSAIGIFASIFFGYLSDIIGRRTQIIINSIMGIGLAY
PYYYLLLQGNESAVIGAIFLGGLLIYYFAGAITPAFLVESFPPMVRYTAISFSYQVGVGFIGGLTPFILTYLVSATSNIF
SPVYFTILTGIVVLILALLLKETKGRIYEGEEILKQEY
>Mature_438_residues
MVSEKTKAIIASSFGMALEWYDFFIYTFVATVVSKLFFPSSNPIASLLAFYLTFFIGFLGRPLGGIVFGYLGDKVGRKYS
LVFTILLTGLSVFFVGLLPTYYQIGLLAPILLAILRFLTGVALGGEWGGAFSLTSEYVNPGRRGLYSGILQATVSIANLL
ATGIIFVIIIIMGQTGFDNFGWRILFYSGLLIAIVGLLIRLRIEDSPVFRKLREDRKILKNPISEAFRKYWKLILANLFI
VGIINGAWYYTNFAYSVGYATTIAQRFNMPHVSLQTIDFAIFLVSAIGIFASIFFGYLSDIIGRRTQIIINSIMGIGLAY
PYYYLLLQGNESAVIGAIFLGGLLIYYFAGAITPAFLVESFPPMVRYTAISFSYQVGVGFIGGLTPFILTYLVSATSNIF
SPVYFTILTGIVVLILALLLKETKGRIYEGEEILKQEY

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=458, Percent_Identity=34.2794759825328, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=29.9771167048055, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=33.1775700934579, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788942, Length=421, Percent_Identity=28.0285035629454, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082404, Length=391, Percent_Identity=23.2736572890026, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=24.1071428571429, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322632, Length=388, Percent_Identity=24.7422680412371, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48554; Mature: 48554

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MVSEKTKAIIASSFGMALEWYDFFIYTFVATVVSKLFFPSSNPIASLLAFYLTFFIGFLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
RPLGGIVFGYLGDKVGRKYSLVFTILLTGLSVFFVGLLPTYYQIGLLAPILLAILRFLTG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALGGEWGGAFSLTSEYVNPGRRGLYSGILQATVSIANLLATGIIFVIIIIMGQTGFDNF
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GWRILFYSGLLIAIVGLLIRLRIEDSPVFRKLREDRKILKNPISEAFRKYWKLILANLFI
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIINGAWYYTNFAYSVGYATTIAQRFNMPHVSLQTIDFAIFLVSAIGIFASIFFGYLSD
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGRRTQIIINSIMGIGLAYPYYYLLLQGNESAVIGAIFLGGLLIYYFAGAITPAFLVES
HHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPPMVRYTAISFSYQVGVGFIGGLTPFILTYLVSATSNIFSPVYFTILTGIVVLILALLL
CCCHHHHHHHEEEHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETKGRIYEGEEILKQEY
HHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]