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The map label for this gene is ppsA-2 [H]

Identifier: 15899536

GI number: 15899536

Start: 2581577

End: 2582515

Strand: Reverse

Name: ppsA-2 [H]

Synonym: SSO2820

Alternate gene names: 15899536

Gene position: 2582515-2581577 (Counterclockwise)

Preceding gene: 15899537

Following gene: 15899530

Centisome position: 86.31

GC content: 35.78

Gene sequence:

>939_bases
ATGAACTATACATACTTACTAGACGAAGTTAGTTTGTCAATGGTCTCGATTGTGGGTAGAAAAAGCGCTTATCTAGGCGA
ATTATATAAGATGGGATTTAACATCCCTAAAGGGTTCATAATATCATCAAGAGGAGTAAATGAAGCGATTAAAGATCTTG
ATGACGAAATAAGAGGAATACTATCCTCCGTAAATTTAAACGATACGACGGATCTGGAAAAGAGAAGCGAAATGATAAAA
AGCATGATTATAGCATCGAAATTACCAAACGAAATGGAAAAAGAAATATATGAAAGGTTCTCTCAATTAGGTTCAAAATA
CGTTGCTGTAAGGGCAACAGCTACATCCCCCTTAAGTGGTGCTAGTTTTGCTGGTGAATACGAAACAGACTTATTTGTTA
CTCAGGAAAATCTCATTCCAAGTATTAAGAGAGTTATCGCGTCGTATTTTAATCCCAGAGCGATAGCTTATAGGATCTTA
ACTCATAATGAAGCAGGGATGGCAATTCTTGTCCAAACAATGATAAACCCAGTTAGTGCAGGTACAGCATTCTCAATACA
CCCCATAACCGAAGAACCAGATTACGTAGTCATAGAGTCATCTTTTGGTCTTGGAGAAAGCGTAACTAAAGGTATGGTTA
CTCCAGATCAATACGTAGTAAGTAAGGCTACAAGATCATTAGTAAGTAAAAGAATTTCTGAAAAAGTCATGAAATTAACT
TATGATTTCGCCGAGAAAAAGATAAAGAGCATTGAACTAAGTAAGGAAGAAGCATTGGCTGAGAGCTTAAGTGATAATGA
TGCTATAAGAATTGCAAACATGGCTATAGCCATAGAAAGTATTTTCAAGAGAAATATAAACATTGAGTGGGCAATAGAGG
ACAAAAAAGTGTACTTATTGGAAGTTAGAGGAATAAGGCGATTGTATCCCGAATTTTAA

Upstream 100 bases:

>100_bases
AGTTTAAACATTTACACTCATAGTGAAAGGATATATTAGATTAACTTTTACAACATAAATAATAGGTAGTATTTTAAGCC
TATTTAACAAAATGTAATTT

Downstream 100 bases:

>100_bases
CCATACCTTAATGCTATGCCTAACCCATATCTTGGATACTTCCATAAGATGTTAGAATAGGGACAAGCAATTCTACAAGC
GCCACACTCAACACAATTCT

Product: phosphoenolpyruvate synthase (ppsA-2)

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase; Mja pepA intein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKRSEMIK
SMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAILVQTMINPVSAGTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLT
YDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLLEVRGIRRLYPEF

Sequences:

>Translated_312_residues
MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKRSEMIK
SMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAILVQTMINPVSAGTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLT
YDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLLEVRGIRRLYPEF
>Mature_312_residues
MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKRSEMIK
SMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAILVQTMINPVSAGTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLT
YDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLLEVRGIRRLYPEF

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DOD-type homing endonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=323, Percent_Identity=30.3405572755418, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17564524, Length=304, Percent_Identity=23.3552631578947, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR003587
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 34976; Mature: 34976

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGI
CCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LSSVNLNDTTDLEKRSEMIKSMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSG
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
ASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRILTHNEAGMAILVQTMINPVSA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCHHHHHHHHHCCCCC
GTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLT
CCEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLL
HHHHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
EVRGIRRLYPEF
EECHHHHHCCCC
>Mature Secondary Structure
MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGI
CCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LSSVNLNDTTDLEKRSEMIKSMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSG
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
ASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRILTHNEAGMAILVQTMINPVSA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCHHHHHHHHHCCCCC
GTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLT
CCEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLL
HHHHHHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
EVRGIRRLYPEF
EECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]