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The map label for this gene is abfD-2 [H]

Identifier: 15899454

GI number: 15899454

Start: 2492589

End: 2494097

Strand: Direct

Name: abfD-2 [H]

Synonym: SSO2738

Alternate gene names: 15899454

Gene position: 2492589-2494097 (Clockwise)

Preceding gene: 15899451

Following gene: 15899457

Centisome position: 83.3

GC content: 39.23

Gene sequence:

>1509_bases
ATGGTTCTAAGAACTGGGGAAGAGTACGTTAATGCAATTAGGCATAGAAATAAGGTTGAAATTTACGTTATGGGGAAGGA
AGTAAAGGATGTAACAGAGCATCCTTTCTTAAAGCCTTCAGTACTTGCATTTAAAGCAACTTATGATGCAGCCCATGAAG
AGGATAGTGAATCTTTAGCTAGAGCATGGAGTTCCTTCATTAATGAAGAGGTAAATAGATTCACCCATATCCATAGATCT
CCAGAGGATTTGGCAGCTAAGGTTAAACTTCTGAGAAAGATCAGTCATAAAACGGGAGCGTGTTTCCAAAGGTGTGTAGG
GTGGGACGCTCTAAATGCAATATACGTTACCTCTAGACTAATGGCGGAAAGGGGTAAAAAGGAGTATTATGAGAGATTTG
TGGAATATTTGAAATATGTACAAAAGAATGATGTAGCGTTAGCTGGAGCCATGACCGATGTTAAAGGTATAAGAACGTTG
AGACCTCATGAGCAACCTCATGCTTATTTAAGAGTAACCCAGGTTACTAAAGACGGTATTTACGTTTCTGGTGCTAAGGC
GAATATAACTGGAATAGGAGCTGCTGAAGAAATTGTGGTTATGCCAACTAGAGCGATGAGTGAGAAAGATATGGATTTTG
CGGTAGCTTTTTCTATACCATTAGATACTGAGGGTATTAAAGTTATAATAGGAAGGCAAGTTAATGATGGGAGAAGGTTA
GAAGAAGGGGAGATAGATGGATTACCCTACCGTACCAATCATGAGGGCTTGGTAATATTTGATAACGTCTTCGTTCCAAT
GGAAAGAGTGTTTATGTTAGGGGATTGGCAGATGAGCGGAATCCTAGTTGAAATTTTCAGCTCTTATCATAGGCAAGGTT
ATGGAGGTTGTAAGTCGGGATTGGGGGATGTGATAATTGGTGCAGCATATAATTTAGCTAAGCAAATTGGAGTCGAAAAA
GCTCCACATATTCAGAATAAGCTAAATGAAATGGTTTTATTGAATGAGAACATGTATGGAAGTGGCATTGCTGCCAGTCT
AGATGCAGTTAAGTTATGTGAAGATGGCTGCTGGTGGGTTAATCCCATGTTTGCAAACGTTACTAAGCAATTAGTCACGA
GATTTCCTTATGAAATTACTAAGTTTTCAACTGATATTGCAGGAGGTATAATAGGTACTGCTCCAAGTGAGTGGGATTTG
AAAAACCCTAAACTTAAACCATATATTGAGAAGTACCTACAAGGTATGATGGACTATAGTACGGAAGATAGGCTAAGAAT
GGTAAGATTATTAGAAAATGTAGGCTTTAGTGTGGCGTTTCTAATTGAATCGGTTCATGGAGCAGGTAGCCCAGAAGCTC
AGAGAATTGCCATAAATAGGGTCTACGACTATGAATATGCTGAGAAGATTGCTAAATACTTGTCGCAAATAGAGAAGAGA
ATTGACTTTAAAGAAAATGCAGAGCCTTGGAGGAAGACAGACACGGAGAAGATAGCTAGTAGTGGCTGA

Upstream 100 bases:

>100_bases
TGTGTTGGTTAGAATTTTGAAATGGTTAGTCATAAAGTTTTTATAATTATATTTATTGGCATATATATAACTTTATAAAC
CAGCTCTGCTACTATATAAT

Downstream 100 bases:

>100_bases
ATCCTTTTTCATGTTAATAGAGATCTTTTTATTTCCATTTTTCTTATTATTTTTTAGTGATTCCAATCCAATAGAGGCGA
TGCACGAAGGGTTGGTTGAT

Product: 4-hydroxybutyryl-CoA dehydratase (abfD-2)

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLARAWSSFINEEVNRFTHIHRS
PEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRLMAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTL
RPHEQPHAYLRVTQVTKDGIYVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRL
EEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSGLGDVIIGAAYNLAKQIGVEK
APHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWVNPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDL
KNPKLKPYIEKYLQGMMDYSTEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKR
IDFKENAEPWRKTDTEKIASSG

Sequences:

>Translated_502_residues
MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLARAWSSFINEEVNRFTHIHRS
PEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRLMAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTL
RPHEQPHAYLRVTQVTKDGIYVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRL
EEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSGLGDVIIGAAYNLAKQIGVEK
APHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWVNPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDL
KNPKLKPYIEKYLQGMMDYSTEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKR
IDFKENAEPWRKTDTEKIASSG
>Mature_502_residues
MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLARAWSSFINEEVNRFTHIHRS
PEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRLMAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTL
RPHEQPHAYLRVTQVTKDGIYVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRL
EEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSGLGDVIIGAAYNLAKQIGVEK
APHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWVNPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDL
KNPKLKPYIEKYLQGMMDYSTEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKR
IDFKENAEPWRKTDTEKIASSG

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 56727; Mature: 56727

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLA
CCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEEHHCCCCCCCHHHHH
RAWSSFINEEVNRFTHIHRSPEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
MAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTLRPHEQPHAYLRVTQVTKDGI
HHHCCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCEEEEEEEEECCCE
YVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRL
EEECCCCCEEECCCCCCEEEECCHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCC
EEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSG
CCCCCCCCCEECCCCCEEEECCCHHCHHHHHEECCCHHHHHHHHHHHHHHHCCCCCHHCC
LGDVIIGAAYNLAKQIGVEKAPHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
NPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDLKNPKLKPYIEKYLQGMMDYS
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCC
TEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKR
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHHHHHHHHH
IDFKENAEPWRKTDTEKIASSG
CCCCCCCCCHHCCCHHHHHCCC
>Mature Secondary Structure
MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLA
CCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEEHHCCCCCCCHHHHH
RAWSSFINEEVNRFTHIHRSPEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
MAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTLRPHEQPHAYLRVTQVTKDGI
HHHCCHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCEEEEEEEEECCCE
YVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRL
EEECCCCCEEECCCCCCEEEECCHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCC
EEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSG
CCCCCCCCCEECCCCCEEEECCCHHCHHHHHEECCCHHHHHHHHHHHHHHHCCCCCHHCC
LGDVIIGAAYNLAKQIGVEKAPHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
NPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDLKNPKLKPYIEKYLQGMMDYS
CHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCC
TEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKR
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHHHHHHHHH
IDFKENAEPWRKTDTEKIASSG
CCCCCCCCCHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]