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The map label for this gene is gspE-2 [C]

Identifier: 15899403

GI number: 15899403

Start: 2440385

End: 2441932

Strand: Reverse

Name: gspE-2 [C]

Synonym: SSO2680

Alternate gene names: 15899403

Gene position: 2441932-2440385 (Counterclockwise)

Preceding gene: 15899404

Following gene: 15899402

Centisome position: 81.61

GC content: 35.4

Gene sequence:

>1548_bases
ATGGTACTTATTGGTAAAAAGAAATCACACAATAAATTTGAAGAATTATCCTATTATCTTCAATCACTTGATGAATCTGG
AATAATATCCCTTAAAGCTAGCCTAGATAACCGAAATATCGTAGAGCATTATACACTAAATAGCTTTGACGTTGAAATTT
ACATTGCCGAAAAGGATGGAATTGGCTTCTACTTAGTAAATGAACCACAACTTGATCAGAGAGAAAACAAAATCTTATTA
GCAATACTCGATGGCTTGATATACTCCGCATCAACCGCCGTATCGAACAAACAAATTGACTTAGAAAAGCTACAAGAAGA
TGTATTAAAAATTTCAGCCAAATTAGGAGTAATAGGCGACGTGAAGAGAAACTTAAAGAAGTATATGTACTATATCACTA
GAGAAATAAAATATTCGATATTACAAGCACCAATGAGTGATCCTTTCGTCGAAGAGATAGAACTAGTATCTCCGACTACA
CCAATTTCAGTAGTACATAGTAGACATAGTGAATGGCCTAGATTGGAGACTAACATCGTTTTAGGGAGCGAATTAAAGGT
AAGGAGATTAGTAGAGAGGCTGGCATCGCTAGGTGGGAGAAGTGTAAGTACAGCTACACCACTACAAGATTTCATGTTAC
CGGAGGGACATAGGGTCGCTGTGAGTTACGGTGAAGAAATAAGTAGGGGAACGACATTTAATATAAGAAAATTCCCTGAA
AAACCTCTAACGATAATTGATTTGATATATAAGTATGGGACATTAAGCGATTTAATGGCAGTTTACTTGTGGATAATTGC
AGAAGCTAAGTTATTTACATTTCTCGTAGGCCCTACTGGTAGTGGAAAGACTACGGCATTGAACGCATTATTGATGCTAC
TTAATCCGTTAGCGAAATATTTAACTATTGAGGATACACCAGAACTAAAATTGCCACATAAATATTGGATACAATTCTAT
ACTAGGCCATCAAACTATGAAGGAAGTAAGGATATCAGTTATTATGAGCTAGTCAGACTTTCCTTAAGATACAGACCAGA
TTACATTATAGTGGGGGAAGTTAGAGGTAAGGAAATAGAATGGTTAGTTCAAGCTGTCGCCAGTGGTCATGGTGGACTTA
CAACCTTTCACGGATCTAATCATACTGACCTAATAACAAGAATTAGCGGATTATTAGGTCCAGAGCTCTCATTACAATTC
AAACAACTAATTTCAGTAATCGCAATCATAAAAAGAATTGAAACTGAGGATAGAAAGATGAATAGGAAAATGATATCTAT
CGTCGAAAATGATGGAAATGGATTTAGGGAAGTATTTAGATATGACTATGAAAGAAAGAACTTTGTTCCAGATAATTTAA
AAGAAATTACTAGTATACAGCTAGAAAGATCTAGAGAACTATTAGGCTGGAGTAGGGATAAGTTGTATAGGGAGATTGAA
AACAGATTACTACTAATAAGAGGGCTTGCCGAAAAGGGAGTTTATGACTATGACACATTAGCAAGAGAGTTAGTCAAGTA
TTATACAAATGGTGATAAGAGTGACTGA

Upstream 100 bases:

>100_bases
TGTTAAGTACGATAATGGGCAAACCTATGCTACATCAGTGGAAGTTATCGACCAATAAGTAACAATAAAGTATTTTATTT
TTATTTTTTTAAATAGGTAT

Downstream 100 bases:

>100_bases
GAGAAGAGTTAAATTAGCTAGTATAATCTCATACGATTTCTTATTTCTAGTTTTACTATCAGGCTTACTAAACTTAATTA
TTACAATATTTAGAGAACGT

Product: Type II secretion system protein, virB homolog (gspE-2)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MVLIGKKKSHNKFEELSYYLQSLDESGIISLKASLDNRNIVEHYTLNSFDVEIYIAEKDGIGFYLVNEPQLDQRENKILL
AILDGLIYSASTAVSNKQIDLEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSILQAPMSDPFVEEIELVSPTT
PISVVHSRHSEWPRLETNIVLGSELKVRRLVERLASLGGRSVSTATPLQDFMLPEGHRVAVSYGEEISRGTTFNIRKFPE
KPLTIIDLIYKYGTLSDLMAVYLWIIAEAKLFTFLVGPTGSGKTTALNALLMLLNPLAKYLTIEDTPELKLPHKYWIQFY
TRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHTDLITRISGLLGPELSLQF
KQLISVIAIIKRIETEDRKMNRKMISIVENDGNGFREVFRYDYERKNFVPDNLKEITSIQLERSRELLGWSRDKLYREIE
NRLLLIRGLAEKGVYDYDTLARELVKYYTNGDKSD

Sequences:

>Translated_515_residues
MVLIGKKKSHNKFEELSYYLQSLDESGIISLKASLDNRNIVEHYTLNSFDVEIYIAEKDGIGFYLVNEPQLDQRENKILL
AILDGLIYSASTAVSNKQIDLEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSILQAPMSDPFVEEIELVSPTT
PISVVHSRHSEWPRLETNIVLGSELKVRRLVERLASLGGRSVSTATPLQDFMLPEGHRVAVSYGEEISRGTTFNIRKFPE
KPLTIIDLIYKYGTLSDLMAVYLWIIAEAKLFTFLVGPTGSGKTTALNALLMLLNPLAKYLTIEDTPELKLPHKYWIQFY
TRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHTDLITRISGLLGPELSLQF
KQLISVIAIIKRIETEDRKMNRKMISIVENDGNGFREVFRYDYERKNFVPDNLKEITSIQLERSRELLGWSRDKLYREIE
NRLLLIRGLAEKGVYDYDTLARELVKYYTNGDKSD
>Mature_515_residues
MVLIGKKKSHNKFEELSYYLQSLDESGIISLKASLDNRNIVEHYTLNSFDVEIYIAEKDGIGFYLVNEPQLDQRENKILL
AILDGLIYSASTAVSNKQIDLEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSILQAPMSDPFVEEIELVSPTT
PISVVHSRHSEWPRLETNIVLGSELKVRRLVERLASLGGRSVSTATPLQDFMLPEGHRVAVSYGEEISRGTTFNIRKFPE
KPLTIIDLIYKYGTLSDLMAVYLWIIAEAKLFTFLVGPTGSGKTTALNALLMLLNPLAKYLTIEDTPELKLPHKYWIQFY
TRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHTDLITRISGLLGPELSLQF
KQLISVIAIIKRIETEDRKMNRKMISIVENDGNGFREVFRYDYERKNFVPDNLKEITSIQLERSRELLGWSRDKLYREIE
NRLLLIRGLAEKGVYDYDTLARELVKYYTNGDKSD

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=150, Percent_Identity=35.3333333333333, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59090; Mature: 59090

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLIGKKKSHNKFEELSYYLQSLDESGIISLKASLDNRNIVEHYTLNSFDVEIYIAEKDG
CEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCEEEEEEEEECCC
IGFYLVNEPQLDQRENKILLAILDGLIYSASTAVSNKQIDLEKLQEDVLKISAKLGVIGD
CEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHH
VKRNLKKYMYYITREIKYSILQAPMSDPFVEEIELVSPTTPISVVHSRHSEWPRLETNIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
LGSELKVRRLVERLASLGGRSVSTATPLQDFMLPEGHRVAVSYGEEISRGTTFNIRKFPE
ECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHCCCCEEEECCCCC
KPLTIIDLIYKYGTLSDLMAVYLWIIAEAKLFTFLVGPTGSGKTTALNALLMLLNPLAKY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LTIEDTPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIE
EEECCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEECCCEEEEECCCCCHHH
WLVQAVASGHGGLTTFHGSNHTDLITRISGLLGPELSLQFKQLISVIAIIKRIETEDRKM
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRKMISIVENDGNGFREVFRYDYERKNFVPDNLKEITSIQLERSRELLGWSRDKLYREIE
HHHEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NRLLLIRGLAEKGVYDYDTLARELVKYYTNGDKSD
HHEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVLIGKKKSHNKFEELSYYLQSLDESGIISLKASLDNRNIVEHYTLNSFDVEIYIAEKDG
CEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCEEEEEEEEECCC
IGFYLVNEPQLDQRENKILLAILDGLIYSASTAVSNKQIDLEKLQEDVLKISAKLGVIGD
CEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHH
VKRNLKKYMYYITREIKYSILQAPMSDPFVEEIELVSPTTPISVVHSRHSEWPRLETNIV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
LGSELKVRRLVERLASLGGRSVSTATPLQDFMLPEGHRVAVSYGEEISRGTTFNIRKFPE
ECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCHHCCCCEEEECCCCC
KPLTIIDLIYKYGTLSDLMAVYLWIIAEAKLFTFLVGPTGSGKTTALNALLMLLNPLAKY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LTIEDTPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIE
EEECCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEECCCEEEEECCCCCHHH
WLVQAVASGHGGLTTFHGSNHTDLITRISGLLGPELSLQFKQLISVIAIIKRIETEDRKM
HHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRKMISIVENDGNGFREVFRYDYERKNFVPDNLKEITSIQLERSRELLGWSRDKLYREIE
HHHEEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NRLLLIRGLAEKGVYDYDTLARELVKYYTNGDKSD
HHEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]