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The map label for this gene is tpiA

Identifier: 15899321

GI number: 15899321

Start: 2358713

End: 2359396

Strand: Direct

Name: tpiA

Synonym: SSO2592

Alternate gene names: 15899321

Gene position: 2358713-2359396 (Clockwise)

Preceding gene: 15899319

Following gene: 15899323

Centisome position: 78.83

GC content: 37.43

Gene sequence:

>684_bases
GTGAAACCTCCTATTATTATAATAAACTTTAAGGCATACGAGAACTCGTTTGGTGATAAAGCAGTAAATTTAGGTAAAAA
AATAGAAAAAATAAGTAAAGAGTACTCAGTTGAAATCATATTGTCAACACCAGCCACCATGATATATAGAATGTCTCAGG
AAGTAGATTTACCAATTTATGCTGAACATGTAGACCCAGTACCTTTAGGAGCATTTACTGGAGCGATATTACCAGAAATG
GTAAAAGATGCAGGTGCTAAGGGTACTTTAATAAATCACAGTGAGAGACGTCTAAGAGCTGATGAAATAGATGATGTGCT
AAAAAGGACCAAAAAATTAGGTTTAAAGAGTATATTATGTGTTGACAGATATGAATTGGTATATCCCTTTAGTTTGCTGA
AACCTGACGCAATTTTAATAGAGCCCCCAGAATTGATAGGAACTGGTGTCTCAGTTTCGAAGGCTAAACCTGAGGTAATA
ACTAGAGCTGTAGATGAGATAAGAAAGTCCGAGGGAATATATCTAATAGCAGGGGCTGGAATTACTACTGGTGAGGATGT
TTATAAGGCGTTGAAGCTTGGAGCTCATGGAATAGGTGTAGCAAGTGCTGTTATGAAGGCTAAAGAGCCTGAAAAGGTTG
TTGAGGACTTTATTACAAGTGCATTGAGGGCGATCTCTTCGTAG

Upstream 100 bases:

>100_bases
TTTTTATCTCGTTGTCTATGAGTATTGCTACTGCAGTCTTTCTTCTTACTGCCAGATCGATTCCACAATACATATTTATT
TCCCTTATTATCTTCTTATT

Downstream 100 bases:

>100_bases
CTATTTTCAATAGGTATATGTAAAGTTCTGGAAAATTATTTTTACAAATGTCCTTTATTTCATTGTTCTCAAATCTAATT
AACGAGAAATATACGTAAAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIYAEHVDPVPLGAFTGAILPEM
VKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILCVDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVI
TRAVDEIRKSEGIYLIAGAGITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS

Sequences:

>Translated_227_residues
MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIYAEHVDPVPLGAFTGAILPEM
VKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILCVDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVI
TRAVDEIRKSEGIYLIAGAGITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS
>Mature_227_residues
MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIYAEHVDPVPLGAFTGAILPEM
VKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILCVDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVI
TRAVDEIRKSEGIYLIAGAGITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SULSO (Q97VM8)

Other databases:

- EMBL:   AE006641
- PIR:   E90432
- RefSeq:   NP_343926.1
- ProteinModelPortal:   Q97VM8
- SMR:   Q97VM8
- GeneID:   1454043
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2592
- NMPDR:   fig|273057.1.peg.2349
- HOGENOM:   HBG297256
- OMA:   VEPPELI
- ProtClustDB:   PRK04302
- BioCyc:   SSOL273057:SSO2592-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_A
- InterPro:   IPR013785
- InterPro:   IPR008867
- InterPro:   IPR000652
- InterPro:   IPR022891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF05690 ThiG; PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 24736; Mature: 24736

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 141-141 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIY
CCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCEE
AEHVDPVPLGAFTGAILPEMVKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILC
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCHHHHHH
VDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVITRAVDEIRKSEGIYLIAGAG
HHHHHHHCCHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
ITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIY
CCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHCCCCCCEE
AEHVDPVPLGAFTGAILPEMVKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILC
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCHHHHHH
VDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVITRAVDEIRKSEGIYLIAGAG
HHHHHHHCCHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCC
ITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726