Click here to switch to the map view.

The map label for this gene is glnA-3 [H]

Identifier: 15899288

GI number: 15899288

Start: 2327353

End: 2328642

Strand: Reverse

Name: glnA-3 [H]

Synonym: SSO2554

Alternate gene names: 15899288

Gene position: 2328642-2327353 (Counterclockwise)

Preceding gene: 15899289

Following gene: 15899287

Centisome position: 77.82

GC content: 36.28

Gene sequence:

>1290_bases
ATGAATGTAGAGAAAGAACTTAGAGAAAAGGGCGTGGAAATTCTAAGATTCACTTGGGTAGGGTTAGATGGGTATATTAG
ATCAAAGGGAGCTTATATTGACCATGTAGATGAATTATTGAAAACGGGTATAGGCTTAACAATGGCAATGATGAGCTTTA
CTCCAATGGATTACATAAGTCCCTATGGCACATTTGGACCACAAGATGAAGATGTATTCCTTACTCCAGACCTTAATACA
CTCTCAGTCTTTCCACCATCAGCCATAGTAATATGCAACCTATATAAGCATGGAAATCCATGGAATTATGATCCTAGAAG
TATATTAATTAAAACAATAGAAAAGGCAAAAGAACAATACGATTTCGACTTCAAATCAGCTTTCGAAATAGAGTTCTATT
TAACAAAGGATAAAAAACCATTGGATGATGCTAGGTGTTTCGATCCTTCTGCATTTTATAACAATCAAATAATCCCAGAA
ATTGCTAAAACGTTAAGGCAAATTAACATAGAACCAATAAGAATAATAAAGGAATATGGACCAGGACAGTATGAGTTTGA
TATAATGCATAAAGACGCGCTAAGGAGTGCGGATGAAGTTGTTATATTTAAGGAAGTCGCAAGACAAGTAGCAAGTAAAC
ATGGTGTAGAAGCGAACTTTATGCCAAAACCATTTAATAAATTGGCTGGATCTGGACTCCATTTAAACATCAGCTTATGG
AAAGATAATCAGAATTTATTCTATAGCCATAATGATAAGTATGGACTTAGCGACTTAGCCTACAATTTCATAGCTGGTTT
GTTGGAACACGCTAAAGCATTAACCGCAATAGCCTCACCAACGATTAACTCCTATAAAAGACTTGTTCCTGGTTCTTGGG
CACCAACTAAAATAACCTATGGATATAACAACAAGTCCGCCATGATAAGAATACCGACACCATATCCCGGAATGTCACAG
TTAGACAGGAGAATAGAATATAGAGTCCCCGATCCCTCCACAAATCCATATCTTCTCTTAACTGCAGTAATAGAAGCTGG
ATTGGATGGAATAGAAAGAGGATTAAAGCCCCCAGAAGCTGTAAATGAAAACGCGTACTATAGAAAAGATATTGATGATA
TTCCGAGAAATCTCAGAGAAGCTCTAAATGAATTGAGAAAGGACACTAAATTGGTGGAGAGGATAGGTAAAGAAATAATT
GGTGAATTCATAAAGGTTAAGTTAGCTGAAGTAGAGGAATATGAAAGCTTAGTAACTGATTGGGAGTACGAGGTGTATAG
ACGATTATAA

Upstream 100 bases:

>100_bases
GGGGAAAAAGTAAAGTGGTATGAAGAACCAGAAGAAGTAAATACTAACTTTACTCAGCAATGATAATTGTTAATAAACTA
GCAAGTTTATTTTATTATTA

Downstream 100 bases:

>100_bases
TAGATTCCCATGCACATTGGTTCTCGGCAAGGGTAATGAGCGAAAAGGAATTTATAATGGCTTCAGCTGAATCTTGGCTC
GAAAACGAAATAAGTACTGA

Product: glutamine synthetase (glutamate ammonia ligase) (GS). (glnA-3)

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLNT
LSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQYDFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPE
IAKTLRQINIEPIRIIKEYGPGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLW
KDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITYGYNNKSAMIRIPTPYPGMSQ
LDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEAVNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEII
GEFIKVKLAEVEEYESLVTDWEYEVYRRL

Sequences:

>Translated_429_residues
MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLNT
LSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQYDFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPE
IAKTLRQINIEPIRIIKEYGPGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLW
KDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITYGYNNKSAMIRIPTPYPGMSQ
LDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEAVNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEII
GEFIKVKLAEVEEYESLVTDWEYEVYRRL
>Mature_429_residues
MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLNT
LSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQYDFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPE
IAKTLRQINIEPIRIIKEYGPGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLW
KDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITYGYNNKSAMIRIPTPYPGMSQ
LDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEAVNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEII
GEFIKVKLAEVEEYESLVTDWEYEVYRRL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=441, Percent_Identity=25.3968253968254, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1790301, Length=419, Percent_Identity=33.4128878281623, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI87081870, Length=346, Percent_Identity=28.0346820809249, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49238; Mature: 49238

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00181 GLNA_ATP ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYIS
CCCHHHHHHCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHCC
PYGTFGPQDEDVFLTPDLNTLSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQY
CCCCCCCCCCCEEECCCCCEEEECCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHC
DFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPEIAKTLRQINIEPIRIIKEYG
CCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCC
PGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLW
CCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEE
KDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITY
ECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEE
GYNNKSAMIRIPTPYPGMSQLDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEA
EECCCEEEEECCCCCCCHHHHHHHHEEECCCCCCCCEEHHHHHHHHCHHHHHHCCCCCHH
VNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEIIGEFIKVKLAEVEEYESLVTD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WEYEVYRRL
HHHHHHHCC
>Mature Secondary Structure
MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYIS
CCCHHHHHHCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHCC
PYGTFGPQDEDVFLTPDLNTLSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQY
CCCCCCCCCCCEEECCCCCEEEECCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHC
DFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPEIAKTLRQINIEPIRIIKEYG
CCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHCC
PGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLW
CCCEEEEEECHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEE
KDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITY
ECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEE
GYNNKSAMIRIPTPYPGMSQLDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEA
EECCCEEEEECCCCCCCHHHHHHHHEEECCCCCCCCEEHHHHHHHHCHHHHHHCCCCCHH
VNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEIIGEFIKVKLAEVEEYESLVTD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WEYEVYRRL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2575777 [H]