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The map label for this gene is proP [H]

Identifier: 15899263

GI number: 15899263

Start: 2297189

End: 2298469

Strand: Reverse

Name: proP [H]

Synonym: SSO2528

Alternate gene names: 15899263

Gene position: 2298469-2297189 (Counterclockwise)

Preceding gene: 15899264

Following gene: 15899262

Centisome position: 76.81

GC content: 37.08

Gene sequence:

>1281_bases
ATGAAAGATGAAACTAAAGTTTCCATAGCTGCCATGATAGGTATCGCCTTCGAGTTTTATGATTTTTTAATATTTGGTTT
CGTATCGAGTATTCTGGCTAGCTTGTTCTTTCCATCAACCAACAAGATAGTATCTTTGTTAGATACATTAGCAGTTTTCG
CTACGGGATTTGCAGGTAGACCGTTGGGCGCTATAGTATTCGGCCACCTTGGTGACAAGATAGGAAGGAAATATACCCTA
ATTGTAACTATGTCGTTAATGGGGTTATCATCCTTGTTTACTGGTTTATTGCCAAGCTATGCAGTATTGGGAATCTTAGC
TCCTACGTTATTAACAGTTTTAAGGCTTCTCCAAGGTTTTTCTTTAGGAGGCGAATTCGGTGGTGGTATAACATTATCTG
CCGAGTTTGCTAAACCCACTAATAGAGCTTTTTATATAGGAATTGCTCAGATGGCTCAAGGAGTAGGACCCTTAATGGCT
ACTGGATTAATATTCATTTTTAGTAGTATTATGTCTCCTCCTGCTTTTGCCTCTACTGGTTGGAGAATACTTTTCATAAT
TGGAGCATTTATTGCTGTAATTGGAGTAATTATTAGATTAAAAATTTCAGAATCACCAGTTTTCAAGAACGTTAGGGAAA
TGGGCCAGATATCAAAATTCCCCCTTGCTGAAGCATTCAGACTCTACTGGAAGAGAATATTATTAGGTTTAGGATTTATT
ATAGGTGGAACTACGTTAACTTATGCTACTAGTGTTTTCGCGGCTTCTTATTTAGAAACTGTAATAGGAGTACCAGCGAA
GACTGTTTCATTAGCGTTAACAATAGGATATATTGTGGAAGCTATATGTATACTTGCATTTTCACTACTGGCTGATAAGA
TAGGGAGGAAACCAATGATGATAACTACCGCAGTCGGTCTATTAATTCTTGTGTATCCGTACTTCTACTTAATTTCTACC
GGTCAGTTCTCATTAATATTATTAGCTCAAATTTTATACTCAACAATAGGCTCATTTTCAACTGCAGCTTATGCTGCTGC
CTTAACTGAACTGTTTCCAACTAAAGTTAGATATACGGCATTATCCTTCGACTATCATGTAGGAGTTGCAGTTTTTGGAG
GTACAACACCATTTATTGCAAGTTATTTAATATACGCTACAGGTTATAAGCTAGCTCCGGTATATTGGGGTATAGCAGGG
ATGGTAATAACATTAATAGCCTATTTGTTATATAAGGAGACTAAGGGGACAATATTTGAAGGGCAGGAAAGAGTAAGGTG
A

Upstream 100 bases:

>100_bases
TAATTGTGAGTAAATTTTAATTTATTAAGTATTTTGCTCATGTTGCCATCTAACTTAAAATGAGAAAAAAATTTAAGAGA
GTTAAAGATGATGAAAAGTT

Downstream 100 bases:

>100_bases
TAGAATATTGGATTTAGGAATAAAGAATGGTAACGTATTTAATGGTAAAGGAGTTGTGGGAAAGGTTAATATTTACGTAA
AGGATGGTAAAATTGTAGAG

Product: metabolite transport protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGFSLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMA
TGLIFIFSSIMSPPAFASTGWRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFI
IGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMMITTAVGLLILVYPYFYLIST
GQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MVITLIAYLLYKETKGTIFEGQERVR

Sequences:

>Translated_426_residues
MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGFSLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMA
TGLIFIFSSIMSPPAFASTGWRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFI
IGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMMITTAVGLLILVYPYFYLIST
GQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MVITLIAYLLYKETKGTIFEGQERVR
>Mature_426_residues
MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGFSLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMA
TGLIFIFSSIMSPPAFASTGWRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFI
IGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMMITTAVGLLILVYPYFYLIST
GQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MVITLIAYLLYKETKGTIFEGQERVR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=31.2354312354312, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=35.5450236966825, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=31.5294117647059, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=29.3706293706294, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45936; Mature: 45936

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
PLGAIVFGHLGDKIGRKYTLIVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGF
CHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMATGLIFIFSSIMSPPAFASTG
CCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
WRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFI
HHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMM
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ITTAVGLLILVYPYFYLISTGQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEE
LSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAGMVITLIAYLLYKETKGTIFE
EEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GQERVR
CHHHCC
>Mature Secondary Structure
MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
PLGAIVFGHLGDKIGRKYTLIVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGF
CHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMATGLIFIFSSIMSPPAFASTG
CCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
WRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFI
HHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMM
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ITTAVGLLILVYPYFYLISTGQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHEEEE
LSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAGMVITLIAYLLYKETKGTIFE
EEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GQERVR
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]