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The map label for this gene is abfD-1 [H]

Identifier: 15899242

GI number: 15899242

Start: 2271280

End: 2272671

Strand: Reverse

Name: abfD-1 [H]

Synonym: SSO2503

Alternate gene names: 15899242

Gene position: 2272671-2271280 (Counterclockwise)

Preceding gene: 15899243

Following gene: 15899241

Centisome position: 75.95

GC content: 37.57

Gene sequence:

>1392_bases
ATGAGATCAAAAGAAGATTTCCTAAAGTCCTTAAAAGATGGAAGAAATTTGTATTATAGGGGGAAGTTAGTAGAAGATAT
AACAACACATCAGATCTTAAAGACAGCCGCATTGCACGCAGCTAAGTTATATGAATACGCTGATAGAGTCTATGAGGATA
ATAAAATGGGAAAAATGAGCAAGTTCTTTAAGGTACCTTGGACATCTCAAGATTTGCTAGATAGACATAAACTAATTTAC
GATTTAACGATGTATTGTAATGGGGTATTTAACATTTCACAAGCAATAGGAAGTGATGCGATCTTTGCCCTTATGATCAC
GGCAAAACAAGTTGATAGAAAATACGGAACTGATTACTCAAAACGTGTTGAAAAATATTTTGAGAGAGTTGCTAAAGAAG
ATTTAACGTTAGCCACTGCCCAGACTGACGTTAAGGGAGATCGAAGTAAGAGGCCTTCTGAACAAGTTGATCCAGATATG
TATGTTAGAGTAGTTGATGTGAAAAGCGATGGAATAGTTGTTAGAGGAGCAAAGGCTCATACAACTCAATCTGCGGTATC
TGATGAGATTATTGTCATACCAACCAGAGTAATGAGGGATAGCGATAAAGATTACGCAGTAGCCTTTGCGGTTCCAGCTA
ATACTAAAGGTTTGAAGATGTATATTAGACCAATTGATGAAATTGAGGGCAATTCCTCCTCAGTACTCAGTAGAAAAGAT
TATGAGCTAGAAACATTAACCGTCTTCAACGACGTTTTCGTTCCTTGGGATAGGGTATTTTTATTTAAGGAATACGACTA
CGCTGGAACATTGGCTATGCTATTTGCAACCTTCCATAGGTTTACTGCATTATCGTATAGGTCAGCGACCATGAATCTAT
ATTTGGGAGCATCTAAAGTGGCATCTCAAGTAAATGGCATTGAGAATGAAAAGCATGTGAGAGATGATATAGTTGATATA
ATTCTCTACAAGGAAATTATGAGGAGTAGCGCGATAGCTGCGGCTGTGTATCCAGTAAACATGGAGGGTATAGCTGTGCC
CAACCCGCTTTTTACTAATGTTGGTAAATTATACTCCAATATGCATTTCCATGATGTTGTAAGAGATTTAATTGACATTG
CTGGGGGGATAATAGCTACTATGCCCTCTCAAGAAGATTTGGAAAGTGATGAAGGAAAGAATATTGTTAAATATTTAAGG
GGCTCAGTTGATGGAGAGGAAAGAGCAAAAGTGTTAAAACTAGCTAAGGAATTAGGGGCTAGTACGTTTACTGGCTATTT
GCTAACTGGTATGATACATGCGGAAGGTTCTATGGAAGCTAGCAAAATAGAGCTATTCAGAAGTTATAATTTTAAGGAGG
CCGAGAACTTAGTTAAAAGGGTATTAAGCTAG

Upstream 100 bases:

>100_bases
TTTAGGTCAATCAATTTGCGGTATGTTCCCAACCCATATCATAGAGTTATTTCTTTAAGGTAAAGAAAAAGTTAATAGAC
TAACATGAGTTATATTATTT

Downstream 100 bases:

>100_bases
TCTTTTTTCCTTTTGATTTGATAATAGAAAAACATAAAAAGAATAAATAGGAAAATAAATCTATGGAAATCACGTTCTCT
ACACAGCATAATATACAAGC

Product: 4-hydroxybutyryl-CoA dehydratase (abfD-1)

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMSKFFKVPWTSQDLLDRHKLIY
DLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYSKRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDM
YVRVVDVKSDGIVVRGAKAHTTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKD
YELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKVASQVNGIENEKHVRDDIVDI
ILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSNMHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLR
GSVDGEERAKVLKLAKELGASTFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS

Sequences:

>Translated_463_residues
MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMSKFFKVPWTSQDLLDRHKLIY
DLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYSKRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDM
YVRVVDVKSDGIVVRGAKAHTTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKD
YELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKVASQVNGIENEKHVRDDIVDI
ILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSNMHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLR
GSVDGEERAKVLKLAKELGASTFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS
>Mature_463_residues
MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMSKFFKVPWTSQDLLDRHKLIY
DLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYSKRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDM
YVRVVDVKSDGIVVRGAKAHTTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKD
YELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKVASQVNGIENEKHVRDDIVDI
ILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSNMHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLR
GSVDGEERAKVLKLAKELGASTFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 52338; Mature: 52338

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMS
CCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KFFKVPWTSQDLLDRHKLIYDLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYS
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
KRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDMYVRVVDVKSDGIVVRGAKAH
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
TTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKD
CHHHHCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCCC
YELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKV
CCEEEEEEHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHH
ASQVNGIENEKHVRDDIVDIILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHHHHHHHHHHC
MHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLRGSVDGEERAKVLKLAKELGA
CCHHHHHHHHHHHCCCEEEECCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
STFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS
CHHHHHHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMS
CCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KFFKVPWTSQDLLDRHKLIYDLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYS
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
KRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDMYVRVVDVKSDGIVVRGAKAH
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCC
TTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKD
CHHHHCCCCEEEEEHHHHCCCCCCEEEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCCC
YELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKV
CCEEEEEEHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHH
ASQVNGIENEKHVRDDIVDIILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCHHHHHHHHHHHC
MHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLRGSVDGEERAKVLKLAKELGA
CCHHHHHHHHHHHCCCEEEECCCHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
STFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS
CHHHHHHHHHEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]