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The map label for this gene is adh-8 [C]

Identifier: 15899235

GI number: 15899235

Start: 2264548

End: 2265558

Strand: Direct

Name: adh-8 [C]

Synonym: SSO2494

Alternate gene names: 15899235

Gene position: 2264548-2265558 (Clockwise)

Preceding gene: 15899234

Following gene: 15899244

Centisome position: 75.68

GC content: 36.8

Gene sequence:

>1011_bases
ATGAAAGCTGTTATTCTTCCAGCTCATAAACAAGGTTATAGAATTGAGGAAGTACCAGATCCTAAACCGGGTAAGGATGA
AGCCATTATTAAGGTTAACAAGGCTGCACTGTGTTATAGGGACTTATTGCAAATAAAAGGATTCTACCCCAGATCTAAAT
ACCCGTTAATTTTAGGTCATGAAGTTGTGGGAACTGTAGAGGAAGTTGGAGAAGACGTTAAGGATTTTAAGAAAGGGGAT
AGAGTTACGTCAATGCTTTTTGTCCCTGACTGGTCATGTGAATATTGTAAAAGTGGAGAAGAAGTTTATTGTAAGAATAG
GGTTGTATACGCTCAAGAATTAGATGGATTTTTCGCAGAAAAAGCTAAAGTTAAGGCAAGCAGTCTAGTTAAAGTACCTG
AGAGTGTTTCCGATGAGGGAGCTGTTATAGTACCATGTGTTGCTGCAATGGTTTATAGGGGTTTGAAAAAAGCTGGAATA
AAAAAAGGAGAAATTGTATTGGTTACTGGAGCAAGTGGTGGTGTAGGTATTCATGCTATTCAAGTAGCTAAGGCTTTAGG
GGCTAAGGTAATAGGAGTTACTAGTGGGGAAAGCAAGGCTAAGATAGTTTCTAAATATGCTGACTACGTAATTGTAGGTG
AGAAGTTCTCAGAAGAGGCTAAAAAGATAGGTGACGTTTCCATTGTAATTGAAAATGTGGGTCCCTATACGCTAGAAGAA
AGTATGAAGAGTCTGAGGAGTGGCGGTAAGATTATACAGATCGGTAATTTAGATCCTTCTGTAACATTTAATTTAAGGCT
GGGCTATATTATTCTTAAAGACATAAGTTTAATGGGGCATATAGGCGCAAATAAAAATGATATAATAAAAACTCTAAACC
TTGTTAAGGAGGGTAAAATAAAACCTGTTATTGGAAGTGAGGTTAGCTTAGAGAATTTTGGAAAAGCTCTTGACTTGCTT
AATGACAGTAAAAATAGGTACGGAAAAATATTGATCTCAAACTCTTTCTAG

Upstream 100 bases:

>100_bases
GCGCATTTTATGATAAAAGTTAATAATCTTTTTTATTTTAATTTTTGTTTTAAGGCATTTATTAAAAACATTTAAAAAGA
GTCAATAACAAATTAAGACT

Downstream 100 bases:

>100_bases
AACTATTGCCCCACCTTGACCTCCACCAACACACAGCGTTGCAACACCGTAATCCTTTCCTTCTATTATTAATTGCCTAG
CCAAGGTACCCACTAGTCTA

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: Acryloyl-CoA reductase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGHEVVGTVEEVGEDVKDFKKGD
RVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAEKAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGI
KKGEIVLVTGASGGVGIHAIQVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEE
SMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKIKPVIGSEVSLENFGKALDLL
NDSKNRYGKILISNSF

Sequences:

>Translated_336_residues
MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGHEVVGTVEEVGEDVKDFKKGD
RVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAEKAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGI
KKGEIVLVTGASGGVGIHAIQVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEE
SMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKIKPVIGSEVSLENFGKALDLL
NDSKNRYGKILISNSF
>Mature_336_residues
MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGHEVVGTVEEVGEDVKDFKKGD
RVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAEKAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGI
KKGEIVLVTGASGGVGIHAIQVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEE
SMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKIKPVIGSEVSLENFGKALDLL
NDSKNRYGKILISNSF

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the acryloyl- CoA dependent NADPH oxidation and formation of propionyl-CoA. Inactive towards 3-hydroxypropionyl-CoA, NADH and crotonyl-CoA [H]

COG id: COG1064

COG function: function code R; Zn-dependent alcohol dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=338, Percent_Identity=29.585798816568, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI194239674, Length=328, Percent_Identity=28.0487804878049, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI13236495, Length=328, Percent_Identity=28.0487804878049, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI262073058, Length=215, Percent_Identity=31.1627906976744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI71743840, Length=215, Percent_Identity=31.1627906976744, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194239676, Length=187, Percent_Identity=33.1550802139037, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI47519420, Length=236, Percent_Identity=30.0847457627119, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI156523966, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4501939, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4501929, Length=212, Percent_Identity=30.6603773584906, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790045, Length=348, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87081918, Length=284, Percent_Identity=27.8169014084507, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787863, Length=350, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI226510992, Length=287, Percent_Identity=28.2229965156794, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788075, Length=341, Percent_Identity=24.9266862170088, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786825, Length=220, Percent_Identity=31.3636363636364, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790485, Length=346, Percent_Identity=27.7456647398844, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788407, Length=328, Percent_Identity=24.390243902439, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082125, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788073, Length=264, Percent_Identity=28.7878787878788, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786518, Length=270, Percent_Identity=25.5555555555556, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082401, Length=311, Percent_Identity=24.7588424437299, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789651, Length=290, Percent_Identity=27.9310344827586, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988145, Length=192, Percent_Identity=35.4166666666667, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17562582, Length=313, Percent_Identity=25.5591054313099, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17562584, Length=330, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17562876, Length=260, Percent_Identity=30.7692307692308, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17562878, Length=253, Percent_Identity=29.6442687747036, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536829, Length=315, Percent_Identity=27.9365079365079, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25146526, Length=224, Percent_Identity=30.8035714285714, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=226, Percent_Identity=30.5309734513274, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17565904, Length=191, Percent_Identity=31.413612565445, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=337, Percent_Identity=29.3768545994065, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319621, Length=303, Percent_Identity=30.3630363036304, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323961, Length=315, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324486, Length=318, Percent_Identity=28.9308176100629, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322619, Length=332, Percent_Identity=29.8192771084337, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=332, Percent_Identity=29.8192771084337, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=227, Percent_Identity=29.0748898678414, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319520, Length=255, Percent_Identity=28.6274509803922, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319949, Length=298, Percent_Identity=26.8456375838926, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323099, Length=326, Percent_Identity=27.3006134969325, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319258, Length=211, Percent_Identity=29.8578199052133, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323980, Length=307, Percent_Identity=27.3615635179153, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17737897, Length=187, Percent_Identity=32.0855614973262, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137530, Length=190, Percent_Identity=31.0526315789474, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737895, Length=235, Percent_Identity=29.3617021276596, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.3.1.84 [H]

Molecular weight: Translated: 36468; Mature: 36468

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00059 ADH_ZINC ; PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGH
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEEECH
EVVGTVEEVGEDVKDFKKGDRVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAE
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCEEEECCEEEEHHHHCCHHHH
KAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGIKKGEIVLVTGASGGVGIHAI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
QVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEE
HHHHHHCCEEEEEECCCHHHHHHHHHCCEEEECCHHHHHHHHCCCEEEEEECCCCHHHHH
SMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKI
HHHHHHCCCCEEEECCCCCCEEEEEEEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHCCCC
KPVIGSEVSLENFGKALDLLNDSKNRYGKILISNSF
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGH
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEEECH
EVVGTVEEVGEDVKDFKKGDRVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAE
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCEEEECCEEEEHHHHCCHHHH
KAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGIKKGEIVLVTGASGGVGIHAI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
QVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEE
HHHHHHCCEEEEEECCCHHHHHHHHHCCEEEECCHHHHHHHHCCCEEEEEECCCCHHHHH
SMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKI
HHHHHHCCCCEEEECCCCCCEEEEEEEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHCCCC
KPVIGSEVSLENFGKALDLLNDSKNRYGKILISNSF
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]