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The map label for this gene is cobB

Identifier: 15899220

GI number: 15899220

Start: 2249306

End: 2250049

Strand: Direct

Name: cobB

Synonym: SSO2478

Alternate gene names: 15899220

Gene position: 2249306-2250049 (Clockwise)

Preceding gene: 15899219

Following gene: 15899222

Centisome position: 75.17

GC content: 38.31

Gene sequence:

>744_bases
ATGATATACGAGAAAGTAGCTGAAGAACTTATTTCCTCTTCTTACACCATAGCCTTTACCGGAGCCGGTATCAGTACAGC
TTCTGGTATTCCCGATTTCAGAGGTCCTCAAGGATTATGGAAGAAGTATTCACCAGAGTTAGCTAGTATAGAATATTTTG
AAAAAGATCCTAAAAATTTTTGGGGATTCTACTCATTAAGAATGAGGGGATTATTCGAAGCCCAACCTAATAAGGCACAC
TACTCTTTGGCTGAGTTAGAAAAGATGGGCATAATAAAGGTAATAATAACACAAAATATTGACGGTCTACATCAGAAGGC
TGGTTCTAAGAACGTTATTGAGCTTCACGGAACTATGAGGAGGTCATATTGCGTTTTATGCTTAAGGACTTATGATTCGC
TAAACGTCCTAAGTATGATAGAAAAGGGTAATCTTCCACCTAGGTGTGATTGTGGTGGGATAATAAGGCCAGATGTTGTG
CTTTTTGGGGAACCAGTTAAGAACATTTATGAGGCACTAAGTATTGCCTATGAATCTGATCTCGTAATTTCAATTGGTTC
TTCATTAACTGTATATCCAGCCAATTTAATTCCACAGACCGTAAAAGAAAGAGGTGGTAAGCTAATAATTTTAAATATGG
AGGAGACCCCGTTGGATAGTATTGCGGATTACGTTGTAAGGGAACCAGTGGAAATTTCATTGCCCAAGATACTTGAGAAC
GTGAGACAGAAAATACTTTCGTGA

Upstream 100 bases:

>100_bases
TAAAAGCAATATATCTGCAGAGGATCTAAAAGGATGAAAGTTAAACTCAAACCTTTTTTATTTTAAAATCTTATTTACCT
ACTAGTAGTATATTCTAATT

Downstream 100 bases:

>100_bases
ATCTCAGTAGTGTTTTTTAAGTAAAATTTCTGATATGATTGAAGAGGAAGAGGAATTAAAATTAATAAAACAAATATTTT
TAATAATTAATGAGAATGTG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog; ssSir2

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILEN
VRQKILS

Sequences:

>Translated_247_residues
MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILEN
VRQKILS
>Mature_247_residues
MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILEN
VRQKILS

Specific function: Probably has a role in chromatin modulation by deacetylation of the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI6912664, Length=252, Percent_Identity=34.1269841269841, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI300795542, Length=239, Percent_Identity=34.7280334728033, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI13787215, Length=231, Percent_Identity=35.4978354978355, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=32.2834645669291, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI300797597, Length=242, Percent_Identity=30.9917355371901, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=36.3636363636364, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI6912662, Length=243, Percent_Identity=31.6872427983539, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI63054862, Length=202, Percent_Identity=32.6732673267327, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI6912660, Length=207, Percent_Identity=31.8840579710145, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7706712, Length=235, Percent_Identity=31.063829787234, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775602, Length=199, Percent_Identity=36.1809045226131, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI13775600, Length=199, Percent_Identity=36.1809045226131, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI300797705, Length=190, Percent_Identity=35.2631578947368, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI215982798, Length=181, Percent_Identity=35.9116022099448, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI308199517, Length=210, Percent_Identity=40.4761904761905, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541892, Length=219, Percent_Identity=31.5068493150685, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17567771, Length=239, Percent_Identity=33.0543933054393, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=244, Percent_Identity=30.7377049180328, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=246, Percent_Identity=30.4878048780488, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505510, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325242, Length=247, Percent_Identity=30.3643724696356, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645650, Length=250, Percent_Identity=30.4, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648389, Length=189, Percent_Identity=37.037037037037, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571445, Length=277, Percent_Identity=28.8808664259928, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137536, Length=234, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24650933, Length=236, Percent_Identity=27.9661016949153, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28571443, Length=219, Percent_Identity=28.310502283105, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28571441, Length=219, Percent_Identity=28.310502283105, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_SULSO (Q97VX5)

Other databases:

- EMBL:   AE006641
- PIR:   H90419
- RefSeq:   NP_343825.1
- ProteinModelPortal:   Q97VX5
- SMR:   Q97VX5
- GeneID:   1453942
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2478
- NMPDR:   fig|273057.1.peg.2248
- HOGENOM:   HBG641281
- OMA:   SSYTIAF
- PhylomeDB:   Q97VX5
- ProtClustDB:   PRK00481
- BioCyc:   SSOL273057:SSO2478-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27594; Mature: 27594

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNF
CCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHH
WGFYSLRMRGLFEAQPNKAHYSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCEEEEHHHHH
RSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVVLFGEPVKNIYEALSIAYESD
HHHHHHEEHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
LVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILEN
EEEEECCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
VRQKILS
HHHHHCC
>Mature Secondary Structure
MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNF
CCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHH
WGFYSLRMRGLFEAQPNKAHYSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCEEEEHHHHH
RSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVVLFGEPVKNIYEALSIAYESD
HHHHHHEEHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
LVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILEN
EEEEECCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
VRQKILS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 11935028