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The map label for this gene is cobA [H]

Identifier: 15899183

GI number: 15899183

Start: 2211482

End: 2212210

Strand: Reverse

Name: cobA [H]

Synonym: SSO2435

Alternate gene names: 15899183

Gene position: 2212210-2211482 (Counterclockwise)

Preceding gene: 15899184

Following gene: 15899182

Centisome position: 73.93

GC content: 35.67

Gene sequence:

>729_bases
ATGAGTGGCAAAGTTTATTTAGTAGGAGCTGGTCCGGGAGACCCTGAATTAATTACTGTTAAAGGCTTAAAGATCCTACA
GAGGGCTGATGTTGTGGTTTACGATAGGCTAATCTCTAAAGAGCTTCTAAATTTTTGTAAGATTGAGGCTGAAAAAATAT
ACGTTGGGAAGAATATAGGGGATTACGTAATACAAGATGAGATAAATGAGTTACTAGTAAAGAAGGCGATGGAAAATAAA
GTAGTTGTTAGATTAAAAGGAGGAGACCCATATGTTTTAGGTAGGGGAGAAGAGGAATGTTTATACGTTATATCCAAAGG
TATAGAGTGTGAGGTAATTCCCGGGATAACTAGTGCAATTGCTGTTCCCGCTTATGCAGGAATCCCTGTGACCAGTAGAA
TTTACTCAGCAAGTGGATTTACTATAATATCTGGAACAAAAGCAGAAGATAAATTGATAGATGAAGATTACATACCAAGA
AAAGGTACGCTGGTAATACTTATGGGTCTTAGGAAGATAGAAAATATGGTTAATATTCTAATAAAATTAAGAAATGAAAG
AGAACCAGTCGCTGTAATTGAAAATGGAACTACAGAAAACCAGAGAGTTTTTACTGGGGAGTTAAAGGACCTAGTTAACA
TTGTCAGAAGTAATAATGTAACGTCTCCAGCGGTTATTGTAATTGGCGAAGTAGTTAAATTTAGGGAATACTTATGGAAA
TTTAAGTGA

Upstream 100 bases:

>100_bases
AATTGCTACTAAACTCAGTAATAATACTTGTGTATGGTATAAAATTTCCAGAAGGTATAGTATTCGCTATTTATATTATG
AGAAAGATAGGGGGGATTTA

Downstream 100 bases:

>100_bases
TAAAGATGAAATGCACTGTGTGTGGAGATGAGATAAGACGGAAGCCTTACTCATTCAATTATAAAGGAAATGTATACTAT
TTCTGTAGCCCCATGTGTAT

Product: uroporphyrinogen III methylase (cobA)

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIGDYVIQDEINELLVKKAMENK
VVVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAIAVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPR
KGTLVILMGLRKIENMVNILIKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWK
FK

Sequences:

>Translated_242_residues
MSGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIGDYVIQDEINELLVKKAMENK
VVVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAIAVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPR
KGTLVILMGLRKIENMVNILIKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWK
FK
>Mature_241_residues
SGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIGDYVIQDEINELLVKKAMENKV
VVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAIAVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPRK
GTLVILMGLRKIENMVNILIKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWKF
K

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=44.6280991735537, Blast_Score=193, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=32.7659574468085, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26831; Mature: 26700

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIG
CCCEEEEEECCCCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
DYVIQDEINELLVKKAMENKVVVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAI
CHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHEEHEECCCCEEEECCCCHHHH
AVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPRKGTLVILMGLRKIENMVNIL
HCCCCCCCCHHHHEECCCCEEEEECCCCHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHH
IKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWK
HHHCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHC
FK
CC
>Mature Secondary Structure 
SGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIG
CCEEEEEECCCCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
DYVIQDEINELLVKKAMENKVVVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAI
CHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHEEHEECCCCEEEECCCCHHHH
AVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPRKGTLVILMGLRKIENMVNIL
HCCCCCCCCHHHHEECCCCEEEEECCCCHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHH
IKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWK
HHHCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHC
FK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]