Click here to switch to the map view.

The map label for this gene is tuF-2 [H]

Identifier: 15899178

GI number: 15899178

Start: 2206949

End: 2208520

Strand: Direct

Name: tuF-2 [H]

Synonym: SSO2429

Alternate gene names: 15899178

Gene position: 2206949-2208520 (Clockwise)

Preceding gene: 15899176

Following gene: 15899180

Centisome position: 73.76

GC content: 37.85

Gene sequence:

>1572_bases
ATGAAATTACCCCGAGAGAATGATGAAGGAAAAGTAGAATATAAGCTTATTCTTTCAAGTGTAACCCCGGATCGTCTTCA
AGAATTAGCTACTCAAATGAAGTATAGGTTAGAGGAAGGTGATGGTGAAGCATTTTACGTTATAGGTGTAAGTGATGAAG
GCGAAATAATAGGGTTATCTAAGGGTCAGCTAGAAGAGAGCATAGCTACGCTGAATATGATCACAAGGTTAGTAAATGCT
AAAATTGTATATAGAAGGGATGTCGAAGTAAGGAGGGATAAATACGTAGCTGAACTTCTAGTTAGGAGATATAAGGAGAA
CCTCCCAGTGGAAGTTAATGTTGCGGTAATGGGTCATGTAAATGCGGGGAAAAGTACTGTTACAGGTGCGTTGGTTCTAG
GAAGACTAGATGATGGAAATGGAGGATTAAGAACAGCTATTGCAAGGCATCTTCATGAAGTCCTATCAGGGAGGACATCT
TCCATTACTTTGAGAGTAATTGGATTTGATGATAGTGGTAAGATAGTAAATTGGCAATTAAAGGATCCACTTGACGAGGC
AGAGACAACGATTAAGAGTACTAAGATAGTGAGGCTTATTGACCTAGGGGGTCACGAAAGATATCTCAGAACCACTCTAA
AAGGGCTATTAGGTTATGAGGTGAATTACGTTATGTTAGTTGTTGGGGCTGATGATGGGTTAAGTATAATGGGCAAAGAG
CATTTAGCCTTAGCTTCAGTATTAAAATTTCCTATATTTGTAGTTATTACTAAAGTAGATAAATATCCGGAAGATAGAAT
AAAAGGCATAGTTAATGATATTAAAAGTGTTCTAAAGATTCCCGGAATTAATAGATTGGCTCTAGAAGTTGAGGATGAGG
ACGATGTAGTAAATTCAATTTTGGCTATAAAGACTAGGCGTGTAGTTCCTATTTTTAAAATATCAAATGTAAGTGGAAAA
GGTTTAGATTTACTTATAAAATTCCTAAACCTATTACCTCCGGAGGCTAAGAATTTAGACGATAGAGGCGCGCCCCTAGT
GTATATCGATGAGATATATAATGTCACAGGGGTTGGCACGGTTGTACTAGGTTCGGTTGTAAGAGGTAAGCTTAACAACA
ATGAGTCCGTTTTAATTGGGCCTAATAAGTTAGGAGAGTTTAAGGAGGTTAAAATAAAAAGCATACAAGTAAATAAGGTA
TTTGTAGATTCCGTTTTAGCTGGGAATATAGCCACGTTTGCTATTCAAGGTATTGAAAAGGAAAGTCTCAGAAAAGGAAT
GGTCATGGTAAAGGGGATACCTAAGGTGGTCAGAAGATTTAAGGCAAGAGTGTTCATACTGCATCATCCAACTACTATTA
GGGAAGGTTATGTTGCGACCCTCCATTCTTATACAATAAGGCAAGCGGTGAAGTTTGAGAAGATCCAGACAGGGTTTTTA
AGAACTGGAGACACCAGTGAAATAGTATTGTATTTCCTATACAGACCAGAATATTTGGAGAAGGGGCAGATTTTCGTATT
TAGAGAAGGTAGAACTAGAGGTGTGGGTATCGTATTGGAACCTATTGGTTAA

Upstream 100 bases:

>100_bases
CTCTTCAAATATTACTTTAGGTTCTTCAGACACTGTCATCCCCTATTAAAATTTATTTAGTTGTTCACAAATTTAATACT
TGTTAAAGTGATTTGATGTA

Downstream 100 bases:

>100_bases
TTTTCCCTACGCTACACTCTATAATCTGCTTTGAGATTTCCTCAGCTTGTGAGCCTATATGCTTCTTTAAACTATTAGCT
AATGGTCCCCCTACTTCATA

Product: elongation factor 1-alpha, putative (tuF-2)

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKLPRENDEGKVEYKLILSSVTPDRLQELATQMKYRLEEGDGEAFYVIGVSDEGEIIGLSKGQLEESIATLNMITRLVNA
KIVYRRDVEVRRDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRVIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASVLKFPIFVVITKVDKYPEDRIKGIVNDIKSVLKIPGINRLALEVEDEDDVVNSILAIKTRRVVPIFKISNVSGK
GLDLLIKFLNLLPPEAKNLDDRGAPLVYIDEIYNVTGVGTVVLGSVVRGKLNNNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFEKIQTGFL
RTGDTSEIVLYFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG

Sequences:

>Translated_523_residues
MKLPRENDEGKVEYKLILSSVTPDRLQELATQMKYRLEEGDGEAFYVIGVSDEGEIIGLSKGQLEESIATLNMITRLVNA
KIVYRRDVEVRRDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRVIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASVLKFPIFVVITKVDKYPEDRIKGIVNDIKSVLKIPGINRLALEVEDEDDVVNSILAIKTRRVVPIFKISNVSGK
GLDLLIKFLNLLPPEAKNLDDRGAPLVYIDEIYNVTGVGTVVLGSVVRGKLNNNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFEKIQTGFL
RTGDTSEIVLYFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
>Mature_523_residues
MKLPRENDEGKVEYKLILSSVTPDRLQELATQMKYRLEEGDGEAFYVIGVSDEGEIIGLSKGQLEESIATLNMITRLVNA
KIVYRRDVEVRRDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRVIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASVLKFPIFVVITKVDKYPEDRIKGIVNDIKSVLKIPGINRLALEVEDEDDVVNSILAIKTRRVVPIFKISNVSGK
GLDLLIKFLNLLPPEAKNLDDRGAPLVYIDEIYNVTGVGTVVLGSVVRGKLNNNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFEKIQTGFL
RTGDTSEIVLYFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5258

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI82546879, Length=508, Percent_Identity=40.5511811023622, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI45593140, Length=531, Percent_Identity=39.7363465160075, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI4503475, Length=402, Percent_Identity=24.6268656716418, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4503471, Length=431, Percent_Identity=24.5939675174014, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI34147630, Length=422, Percent_Identity=25.8293838862559, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI2367247, Length=238, Percent_Identity=26.0504201680672, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17563834, Length=534, Percent_Identity=37.2659176029963, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17565438, Length=533, Percent_Identity=36.7729831144465, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17552884, Length=422, Percent_Identity=25.3554502369668, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17569207, Length=422, Percent_Identity=25.3554502369668, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=426, Percent_Identity=25.1173708920188, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=427, Percent_Identity=23.8875878220141, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922512, Length=528, Percent_Identity=38.2575757575758, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24654852, Length=528, Percent_Identity=38.2575757575758, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221378008, Length=533, Percent_Identity=37.5234521575985, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24644462, Length=533, Percent_Identity=37.5234521575985, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24644464, Length=533, Percent_Identity=37.5234521575985, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24644466, Length=533, Percent_Identity=37.5234521575985, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI28573213, Length=533, Percent_Identity=37.5234521575985, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24652838, Length=430, Percent_Identity=25.1162790697674, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137572, Length=430, Percent_Identity=25.1162790697674, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45553807, Length=412, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI45553816, Length=412, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24651721, Length=412, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17864154, Length=412, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281363316, Length=422, Percent_Identity=24.8815165876777, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17864358, Length=422, Percent_Identity=24.8815165876777, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550900, Length=388, Percent_Identity=26.8041237113402, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 58231; Mature: 58231

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPRENDEGKVEYKLILSSVTPDRLQELATQMKYRLEEGDGEAFYVIGVSDEGEIIGLS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECC
KGQLEESIATLNMITRLVNAKIVYRRDVEVRRDKYVAELLVRRYKENLPVEVNVAVMGHV
CCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTSSITLRVIGFDDSGKIVNWQL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEE
KDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
CCCHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCH
HLALASVLKFPIFVVITKVDKYPEDRIKGIVNDIKSVLKIPGINRLALEVEDEDDVVNSI
HHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LAIKTRRVVPIFKISNVSGKGLDLLIKFLNLLPPEAKNLDDRGAPLVYIDEIYNVTGVGT
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCHHH
VVLGSVVRGKLNNNESVLIGPNKLGEFKEVKIKSIQVNKVFVDSVLAGNIATFAIQGIEK
HHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEEEEHEEEHHHHHCCEEEEEEECCCH
ESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFEKIQTGFL
HHHHCCEEEECCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
RTGDTSEIVLYFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
ECCCCCCEEEEEEECCCHHCCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MKLPRENDEGKVEYKLILSSVTPDRLQELATQMKYRLEEGDGEAFYVIGVSDEGEIIGLS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECC
KGQLEESIATLNMITRLVNAKIVYRRDVEVRRDKYVAELLVRRYKENLPVEVNVAVMGHV
CCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTSSITLRVIGFDDSGKIVNWQL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEE
KDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
CCCHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCCH
HLALASVLKFPIFVVITKVDKYPEDRIKGIVNDIKSVLKIPGINRLALEVEDEDDVVNSI
HHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LAIKTRRVVPIFKISNVSGKGLDLLIKFLNLLPPEAKNLDDRGAPLVYIDEIYNVTGVGT
HHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCHHH
VVLGSVVRGKLNNNESVLIGPNKLGEFKEVKIKSIQVNKVFVDSVLAGNIATFAIQGIEK
HHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEEEEHEEEHHHHHCCEEEEEEECCCH
ESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFEKIQTGFL
HHHHCCEEEECCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
RTGDTSEIVLYFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
ECCCCCCEEEEEEECCCHHCCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA