Click here to switch to the map view.

The map label for this gene is rimK-3 [H]

Identifier: 15899102

GI number: 15899102

Start: 2141705

End: 2142397

Strand: Direct

Name: rimK-3 [H]

Synonym: SSO2346

Alternate gene names: 15899102

Gene position: 2141705-2142397 (Clockwise)

Preceding gene: 15899101

Following gene: 15899106

Centisome position: 71.58

GC content: 37.95

Gene sequence:

>693_bases
TTGGGCTTCATCTCTACTACTGAGCAGTTCATAAAGAGGTTCGATGTATTAAGGGAGCTAGAAAGAAATGGAGTAGTACT
AATGAATAGGCCAGATTCGATGTTGCTAGCCAGGGATAAGTTTGCAAGTTTAATGAGAATGAGAAGGGCAGGTATTCCAG
TTCCAAACACTGCACTAGTAGAAGATCCCTTCGAAGTTATGAGATTAGCAGAAAGATGGGGCGAAGTTGTGATAAAACCT
GTAGTGGGAAGTCTAGGTTTAGGATCCGTTAAGGTTTCAGATCCAGATATAGCGTTTAGAGTAGCTAAGGCTATTTTATC
GGTAAATCAGCCAGTTTACGTTCAAAAATATGTAAAGAAACCAGATAGAGATATTAGAGTGATTGTAATTGGGGATAAAG
TTCTAGGAAGCATATATAGAATATCGAAGAGTGGATGGAAAACTAATATAGCCCAAGGCGCTACAGCACAAGTTTTAATT
CCAGATGCAGAATTAGAAGAAATAAGCTTAAAGAGTGTTAGAGTCCTCGGTTTGGATTATGCTGGGATAGATATTATAGA
AGATGTGGAGAACGGTGGTTATAAGATAATTGAAGTTAATGCAGCACCTTTGTGGGACGGATTTGAGGCAGCTACAAATA
TTAATCCAGCTAAATATATTGTCACGCATTTAATAGAAAAGATTAGGAGATGA

Upstream 100 bases:

>100_bases
TATAAGAATTTCCAAACTAAATGCAGAAATAACCGAGCATGGCATAGAATTTACATATAGTGGAAGGAAGGTTGATATAG
ATGGAGGACTAATAAGGAAC

Downstream 100 bases:

>100_bases
GGAATAGAAAACCGTCTGAAATGTGATGAAGAGCTCTCGCTCCTGATTTAAAGATCGAGCTCTTTCTTGTCCTTTATTGA
TCTAAGTACAAGCATTGTAT

Product: ribosomal protein S6 modification protein (rimK-3)

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MGFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALVEDPFEVMRLAERWGEVVIKP
VVGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKKPDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLI
PDAELEEISLKSVRVLGLDYAGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR

Sequences:

>Translated_230_residues
MGFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALVEDPFEVMRLAERWGEVVIKP
VVGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKKPDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLI
PDAELEEISLKSVRVLGLDYAGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR
>Mature_229_residues
GFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALVEDPFEVMRLAERWGEVVIKPV
VGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKKPDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLIP
DAELEEISLKSVRVLGLDYAGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=210, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI134254462, Length=219, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787076, Length=209, Percent_Identity=33.4928229665072, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 25647; Mature: 25516

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALV
CCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
EDPFEVMRLAERWGEVVIKPVVGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKK
CCHHHHHHHHHHHHHEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHCC
PDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLIPDAELEEISLKSVRVLGLDY
CCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHEEEEECCC
AGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR
CCCHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALV
CCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
EDPFEVMRLAERWGEVVIKPVVGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKK
CCHHHHHHHHHHHHHEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHCC
PDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLIPDAELEEISLKSVRVLGLDY
CCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHEEEEECCC
AGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR
CCCHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]