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The map label for this gene is exoA [H]

Identifier: 15899057

GI number: 15899057

Start: 2099610

End: 2100353

Strand: Direct

Name: exoA [H]

Synonym: SSO2290

Alternate gene names: 15899057

Gene position: 2099610-2100353 (Clockwise)

Preceding gene: 15899056

Following gene: 15899059

Centisome position: 70.17

GC content: 35.08

Gene sequence:

>744_bases
GTGAAGATAGTAAGTTGGAACATTAATGGTTTACGTTCAGCTTTAAAGAAGAATCTAGTAAACTTCCTAGAAAGTAATAC
GTTCGATATTGTAATGTTTCAAGAGACTAGAGGTGATATAGTTCCGCTTGATTTCATTATGATGGGCTATGAAGTAATTT
CATTTCCAGCAAGGAGAAAAGGTTACAGCGGGGTAATGACATTAACCAAAATAAAACCCATAAAAGTGATTAAAGGTCTT
CAAATCAAGGAGTTTGACGATGAAGGGAGAACAGTTAGCTTAGAACTGAAAGACTTCTACCTTATTAACGCGTATTTTCC
TAGAGCTGGAGATAACTTGGAAAGATTGGATTTTAAGCTAAAATTTAATAACGGGATCGAGAACTTCCTTCTTAAATTAA
GAGAAGTTAAACCAGTAATACTTTGCGGAGATTTCAATATTGCACATCAAAGCATTGATGCGGCTTTTTCTGATCCAAAA
ATCCCAGGGCTCACACCACAAGAGAGAGCGTGGTTTACTCATCTTCTCTCTTTAGGTTTCATAGACACTTTTAGATATTT
ACACCCAAATGTTAGAAAATATAGCTGGTGGAGCTATATGGGAAATGCTAGAGAGAAGAATTTAGGACTAAGATTAGATT
ATTGTATTGTGTCAGAAGAGTTAAAAGATAGTATAAAAATGGCTGACATTCTGACAGATGTACAAGGCTCTGATCATGCA
CCAATTATCTTGGAATTAACATAA

Upstream 100 bases:

>100_bases
TTCTTGGCAAGTAATGAAGCGAGATATATTACTGGACAAGTCATAGTTGCGGATGGTGGTAGAATAGACAATTTAACTCA
TTCATTATAATCTTCTACAT

Downstream 100 bases:

>100_bases
GTTATATTCTATAGTAGTATAGATTGCTCTAGCGTTAGTTCTTTGTAATAACATATCATACAAAAGGTTATTTAACCATG
ATGGTATAGTATTTATTTTA

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPIKVIKGL
QIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKLKFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPK
IPGLTPQERAWFTHLLSLGFIDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHA
PIILELT

Sequences:

>Translated_247_residues
MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPIKVIKGL
QIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKLKFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPK
IPGLTPQERAWFTHLLSLGFIDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHA
PIILELT
>Mature_247_residues
MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPIKVIKGL
QIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKLKFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPK
IPGLTPQERAWFTHLLSLGFIDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHA
PIILELT

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=38.671875, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=38.671875, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=38.671875, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI18375507, Length=319, Percent_Identity=30.7210031347962, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=31.6981132075472, Blast_Score=118, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71989536, Length=252, Percent_Identity=38.4920634920635, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=32.1678321678322, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=40.1574803149606, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=40.1574803149606, Blast_Score=187, Evalue=4e-48,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28463; Mature: 28463

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRK
CEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHCC
GYSGVMTLTKIKPIKVIKGLQIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKL
CCCCEEEEEECCHHHHHCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCHHHEEEEE
KFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPKIPGLTPQERAWFTHLLSLGF
EECCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
IDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHA
HHHHHHHCCCCCEEEHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCC
PIILELT
CEEEEEC
>Mature Secondary Structure
MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRK
CEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHCC
GYSGVMTLTKIKPIKVIKGLQIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKL
CCCCEEEEEECCHHHHHCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCHHHEEEEE
KFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPKIPGLTPQERAWFTHLLSLGF
EECCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
IDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHA
HHHHHHHCCCCCEEEHHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCC
PIILELT
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]