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The map label for this gene is treX [H]

Identifier: 15898878

GI number: 15898878

Start: 1908797

End: 1910953

Strand: Direct

Name: treX [H]

Synonym: SSO2094

Alternate gene names: 15898878

Gene position: 1908797-1910953 (Clockwise)

Preceding gene: 15898877

Following gene: 15898879

Centisome position: 63.79

GC content: 37.88

Gene sequence:

>2157_bases
ATGGCATTATTCTTCAGAACTAGAGATAGGCCTCTTCGTCCCGGAGATCCATATCCATTAGGTTCGAATTGGATAGAAGA
TGATGATGGTGTGAATTTTTCATTATTCTCAGAAAATGCAGAAAAAGTCGAATTACTCCTTTACTCACTGACAAACCAAA
AGTACCCAAAGGAGATAATAGAGGTTAAAAACAAAACGGGAGATATTTGGCACGTCTTCGTCCCGGGACTGAGACCCGGG
CAACTTTACGCATATAGGGTTTATGGCCCATATAAACCGGAGTTGGGATTAAGGTTTAATCCAAATAAGGTTTTAATAGA
CCCTTATGCTAAAGCCATAAACGGTAGTGTAATTTGGAACGACGCGGTATTTGGTTATAAGATAGGAGATCAAAATCAAG
ATTTGACCTATGACGAGAGGGATTCCGGCGAATATGTTCCTAAAAGTGTTGTGATTAATCCATACTTTGAGTGGGACGAT
GAGGATTTCATTAAGGGAAAGAAAGTTCCATTAAAGGATACAGTAATTTACGAAGTTCACGTAAAAGGGTTTACAAAACT
TAGGTTGGATTTGCCGGAAAACATAAGGGGAACTTATGAAGGTCTTGCCTCAGAACAAATGATCAGCTATCTCAAAGATC
TAGGGATTACTACTGTGGAACTAATGCCAGTTTTCCATTTTATCGATCAAAGATTTCTGACAGATAAGGGGCTAACGAAT
TACTGGGGGTATGACCCAATAAATTTCTTCTCTCCTGAATGTAGATATTCCAGTACTGGCTGTTTAGGAGGGCAAGTATT
AAGTTTCAAAAAAATGGTAAACGAATTACATAACGCAGGAATTGAGGTTATAATCGATGTAGTTTATAATCACACTGCTG
AGGGGAATCATTTAGGCCCAACGTTAAGTTTCAGAGGTATTGATAATACAGCATATTACATGCTCCAACCAGATAATAAA
AGATATTATTTAGACTTTACCGGAACTGGCAATACGTTAAATCTTAGCCATCCTAGAGTCATCCAGATGGTCTTAGATAG
CTTAAGATATTGGGTTACAGAGATGCACGTAGATGGCTTTAGATTTGACTTAGCAGCAGCTTTAGCCAGGGAATTATACA
GCGTTAATATGTTAAATACGTTCTTCATTGCTCTACAGCAAGACCCAATATTGTCACAAGTAAAACTGATAGCTGAGCCT
TGGGATGTGGGACAAGGGGGATATCAAGTAGGGAATTTCCCATATCAATGGGCAGAGTGGAATGGAAAGTATAGGGATTC
GATAAGGAGATTTTGGAGAGGAGAAGCGTTACCCTATAGCGAGATTGCTAACAGATTATTAGGCTCGCCGGACATTTACT
TAGGTAATAATAAAACACCATTTGCCAGTATAAATTACGTAACTTCTCATGATGGTTTCACATTAGAGGATTTAGTCAGT
TATAATCAAAAACACAATGAAGCGAATGGATTTAACAATCAAGATGGAATGAACGAGAACTATAGTTGGAATTGTGGTGC
AGAAGGACCAACAAATGACCAAAACGTGGTAATATGCAGGGAGAAACAAAAAAGGAACTTTATGATAACGTTACTCGTTA
GTCAAGGAACCCCTATGATATTGGGAGGAGATGAGCTAAGCAGGACACAAAGAGGAAATAATAACGCGTTTTGTCAAGAC
AACGAGATAACTTGGTTTGATTGGAATTTAGATGAGAGAAAATCAAAATTTTTAGAGTTTGTTAAAAAGATGATCCAATT
TTATAGGGCACATCCAGCATTCAGAAGGGAAAGATATTTTCAAGGAAAGAAATTATTCGGCATGCCGTTAAAAGATGTGA
CCTTCTATACTCTAGAAGGTAGGGAAGTTGATGAGAAAACATGGAGTTCCCCGACGCAACTAGTTATTTTCGTGTTGGAG
GGAAGTGTTATGGACGAGATTAATATGTATGGAGAGAGAATTGCTGATGATTCCTTCTTAATAATACTTAACGCAAATCC
CAATAACGTAAAGGTGAAATTCCCAAAAGGTAAATGGGAACTAGTTATCAGTTCTTATTTGAGAGAAATAAAACCAGAAG
AGAGAATCATAGAAGGTGAGAAGGAACTGGAAATAGAGGGAAGGACGGCATTAGTTTATAGGAGGATAGAATTATGA

Upstream 100 bases:

>100_bases
GGAAGAGAGAAGGAGAAGAGAAGAAGTTAAAAGGTTAATAATGCTCTTAGCAATGTTAAGATAAGGTTTTTAAAAAACTA
AATAATAGTTAAGTACAACT

Downstream 100 bases:

>100_bases
TAATAGGTACGTATAGGCTACAGCTCAATAAGAAATTCACTTTTTATGATGTAATAGAAAATTTGGATTATTTTAAAGAA
TTAGGAGTATCACATCTATA

Product: glycogen debranching protein TreX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEIIEVKNKTGDIWHVFVPGLRPG
QLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWNDAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDD
EDFIKGKKVPLKDTVIYEVHVKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTN
YWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNK
RYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEP
WDVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
YNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQD
NEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLE
GSVMDEINMYGERIADDSFLIILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL

Sequences:

>Translated_718_residues
MALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEIIEVKNKTGDIWHVFVPGLRPG
QLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWNDAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDD
EDFIKGKKVPLKDTVIYEVHVKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTN
YWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNK
RYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEP
WDVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
YNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQD
NEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLE
GSVMDEINMYGERIADDSFLIILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL
>Mature_717_residues
ALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEIIEVKNKTGDIWHVFVPGLRPGQ
LYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWNDAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDDE
DFIKGKKVPLKDTVIYEVHVKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTNY
WGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNKR
YYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEPW
DVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVSY
NQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQDN
EITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLEG
SVMDEINMYGERIADDSFLIILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=670, Percent_Identity=42.5373134328358, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789839, Length=370, Percent_Identity=23.5135135135135, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83092; Mature: 82961

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEII
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEEEHHCCCCCCHHHH
EVKNKTGDIWHVFVPGLRPGQLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWN
HHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCCEEEECCHHHHCCCCEEEE
DAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDDEDFIKGKKVPLKDTVIYEVH
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEE
VKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTN
ECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCC
YWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCC
TLSFRGIDNTAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGF
EEEEECCCCCEEEEEECCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCH
RFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEPWDVGQGGYQVGNFPYQWAEW
HHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHCCCCCCCCCCEEECCCCEECCCC
NGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHH
YNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMI
HHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEE
LGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYF
ECCCHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLEGSVMDEINMYGERIADDSFL
CCCEEECCCCCCEEEEEEECCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEE
IILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL
EEEECCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEECC
>Mature Secondary Structure 
ALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEII
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEEEHHCCCCCCHHHH
EVKNKTGDIWHVFVPGLRPGQLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWN
HHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCEECCCEEEECCHHHHCCCCEEEE
DAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDDEDFIKGKKVPLKDTVIYEVH
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEE
VKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTN
ECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCC
YWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCC
TLSFRGIDNTAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGF
EEEEECCCCCEEEEEECCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCH
RFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEPWDVGQGGYQVGNFPYQWAEW
HHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHCCCCCCCCCCEEECCCCEECCCC
NGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHH
YNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMI
HHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEE
LGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYF
ECCCHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLEGSVMDEINMYGERIADDSFL
CCCEEECCCCCCEEEEEEECCCCCHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEE
IILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL
EEEECCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]