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The map label for this gene is gdhA-4 [H]

Identifier: 15898835

GI number: 15898835

Start: 1861881

End: 1863143

Strand: Direct

Name: gdhA-4 [H]

Synonym: SSO2044

Alternate gene names: 15898835

Gene position: 1861881-1863143 (Clockwise)

Preceding gene: 15898834

Following gene: 15898836

Centisome position: 62.22

GC content: 37.37

Gene sequence:

>1263_bases
TTGATGGAAGAAGTTCTTAGTTCGAGTCTGTATACTCAACAAGTCAAAAAGTTATATAAAGTCGGAGAATTATTGGGTCT
TGATAACGAAACTTTAGAAACGCTTTCTCAGCCCGAAAGAATAATTCAAGTTAAGATACAAATTAGAGGGTCTGATGGTA
AATTAAAAACATTTATGGGTTGGAGAAGTCAGCACAACTCGGCTTTAGGCCCATACAAGGGCGGAATTAGGTATCATCCT
AATGTTACACAAGATGAGGTAGAAGCGTTATCTATGATAATGACGTGGAAGAACTCATTACTATTATTACCATATGGAGG
AGGAAAAGGTGGAATTAGAGTAGATCCTAAAAAGTTGACTAAAGAAGAATTAGAACAATTATCGAGAAAGTTTATCCAAG
CTATTTATAAATATTTAGGTAGCGAATTAGATATCCCAGCCCCAGATGTGAACACAGATTCACAAACCATGGCATGGTAC
CTAGATGAGTACATAAAGATAACTGGAAATGTTGATTTCGCAGTATTTACTGGGAAGCCAGTGGAACTAGGTGGAATAGG
TGTTAGATTATACAGTACTGGACTTGGAGTTGCTACCATAGCTAAGGAAGCTGCGAATAAGTTTATAGGAGGTATTGAGG
AAGCTAGGGTAATAATTCAAGGCTTCGGAAATGTTGGGTATTATGCAGCTAAATTCCTAAGCGATATGGGAGCTAAAATA
GTAGGAATTAGTGACTCTAAAGGTGGAGTAATTAATGAGAATGGAATAGATGTTGGAAAGGCTATGGAAATAAAGGAGAA
AACTGGAAGTGTGACAAATTATCCTGAGGGAAGAAAGGTCACAAATGAGGAACTACTAATATCGGACTGTAATATATTAG
TACCTGCAGCGCTTGAAAACGTCATAAATAAGTTTAATGCTCCCAAGATTAAGGCTAAGCTTATTGTTGAGGGTGCAAAT
GGTCCATTGACTGCTGATGCAGATGAAATAATGAGACAAAGGGGCATAGTAGTTGTGCCAGATATACTTGCTAATGCTGG
TGGAGTTGTTGGAAGTTATGTAGAGTGGGCTAATAATAAAATGGGTCAAATAATAAGCGATGAAGAGGCTAAGAAGCTTA
TAGTCGACAGAATGAACAATGCTTTCAATACCCTATACGATTATCATCAAAAGAAATTAGAAGATCACGATCTGAGAACT
GCTGCCATGGCATTAGCTGTGGATAGAGTAGTAAGAGCTATGAAAGCTAGAGGTCTATTATAA

Upstream 100 bases:

>100_bases
TTTATATCGTTTTGACTTTTTCACCTAAAGAAAATTGTGAGTTAAGAAAATTTTAAAGCTATGCTGGAATAAATTTTTAT
TCCGGGAAAACGAATTTATA

Downstream 100 bases:

>100_bases
TATTTTTTCCTTGGGCTTAATCCTAGACCCCTTAAAGAGAAAGATAAGACAAGGTAGGATAATAATGAAATAAAGAATAG
AGCTTGATTAGAGTTCAACT

Product: NAD specific glutamate dehydrogenase (gdhA-4)

Products: NA

Alternate protein names: GDH-2 [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGIRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTKEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWY
LDEYIKITGNVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKI
VGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALENVINKFNAPKIKAKLIVEGAN
GPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNKMGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGLL

Sequences:

>Translated_420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGIRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTKEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWY
LDEYIKITGNVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKI
VGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALENVINKFNAPKIKAKLIVEGAN
GPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNKMGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGLL
>Mature_420_residues
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGIRYHP
NVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTKEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWY
LDEYIKITGNVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKI
VGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALENVINKFNAPKIKAKLIVEGAN
GPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNKMGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRT
AAMALAVDRVVRAMKARGLL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=372, Percent_Identity=37.6344086021505, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=38.7096774193548, Blast_Score=261, Evalue=6e-70,
Organism=Escherichia coli, GI1788059, Length=338, Percent_Identity=33.4319526627219, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17544676, Length=396, Percent_Identity=36.8686868686869, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6324951, Length=396, Percent_Identity=29.7979797979798, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319256, Length=406, Percent_Identity=28.8177339901478, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24649283, Length=371, Percent_Identity=39.0835579514825, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI45553475, Length=339, Percent_Identity=38.3480825958702, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI45549226, Length=339, Percent_Identity=38.3480825958702, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=37.9928315412186, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46064; Mature: 46064

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHH
WRSQHNSALGPYKGGIRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLT
HHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHH
KEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWYLDEYIKITGNVDFAVFTGKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCC
VELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKI
EEECCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEE
VGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALEN
EEECCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHH
VINKFNAPKIKAKLIVEGANGPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNK
HHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHHCCCCHHHHHHHHHCCC
MGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHH
WRSQHNSALGPYKGGIRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLT
HHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHHHH
KEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWYLDEYIKITGNVDFAVFTGKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCC
VELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKI
EEECCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEE
VGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALEN
EEECCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHH
VINKFNAPKIKAKLIVEGANGPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNK
HHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHHCCCCHHHHHHHHHCCC
MGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGLL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726; 1730244 [H]