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The map label for this gene is proP [H]

Identifier: 15898830

GI number: 15898830

Start: 1853782

End: 1855065

Strand: Direct

Name: proP [H]

Synonym: SSO2039

Alternate gene names: 15898830

Gene position: 1853782-1855065 (Clockwise)

Preceding gene: 15898828

Following gene: 15898831

Centisome position: 61.95

GC content: 37.23

Gene sequence:

>1284_bases
ATGAAGGAAGAAACAAAAGTTTCAATCGCAGCAATGATAGGTATAGCATTCGAATTCTATGATTTTTTGATATTCGGATT
CGTAGCTGGCATCTTGGCTAACTTATTCTTCCCATCTGGAAATAAAATAGTGTCGCTTCTTGACACGTTTGCAGTATTTG
CTACAGGCTTTGCTGGTAGACCGCTGGGAGCAATAGTGTTTGGTCATCTAGGTGATAAGATAGGAAGGAAATATACATTA
ATAATTACTATGACATTAATGGGGCTATCGTCATTATTTACGGGTTTATTACCAGGTTACGCTATATTAGGTGTTTTAGC
ACCCACGTTGCTGACGATTTTGAGATTACTTCAAGGATTTTCACTAGGAGGCGAATTTGGAGGAGGAATTACTCTCTCAG
CGGAATTTGCAGAACCTGGGAAAAGAGCTTTTTATACCGGAATTGCTCAAATGGCCCAAGGTATAGGCCCGCTAATGGCT
ACTGGATTAATATTCGTCTTTAGTAGTATTATGTTACCTAGTGCTTTCGCATCTATTGGTTGGAGAATACTTTTCATAAT
TGGCGCATTTATCGCAGTAATAGGAGTCATGATAAGACTAAAAATTTCTGAATCACCAGTATTCAAAAAAGTTAAGGAGA
AGAATGAGATTTCTCATTTTCCACTAGTTGATGCTTTCAGAATTTATTGGAAAAGGATACTATTGGGTTTAGGATTCATA
ATAGGTGGTACTACAATGACATATGCTACTAGTGTTTTCGCATCATCATATTTAGAAAATGTGATAGGAGTACCTGCACG
ACTCGTTTCATTAGCCTTGACTATAGGATACATCGTTGAGGCAATATCTATACTCTTGTTCGCAATGTTGGCTGATAAAG
TAGGGAGAAAGCCATTAATGGTTGCAACTGCAGTAGGTTTATTAATCCTCGTATACCCGTATTTCTTATTAATCTCTACT
GGCCAATTCTCACTAATATTGTTAGCTCAAATAATGTACTCCGTTATAGGTTCAATGTCAACTGGTGCATATGCAACTGC
CTTAACTGAGTTATTCCCAACCAAAGTAAGATATACAGCCTTATCCTTCGACTACCATGTGGGAGTTGCGGTATTTGGAG
GAACCACGCCATTCATTGCAAGCTATCTGATTTACGCTACTGGTTATAAATTAGCACCTGTTTATTGGGGAATAGCTGGA
ATGATCTTAACATTAATAGCTTATTTATTGTATAAAGAAACTAAAGGAACAGTATTTGAGGGACAAGAAGTATTGAAAAG
ATAA

Upstream 100 bases:

>100_bases
AATTTTTCAGTTATATTGTAAAAGTCTCTTCATTATGATAGAATACTGTTTAACTTTTAATGAGTAAAAAATTGAAGGAA
GAAACAAACGATTGTAAAAT

Downstream 100 bases:

>100_bases
GGGATAATCATGGAGCTAGCCATAAGAAACGGGATCGTTTTTAACGGAAAAGAAATAATAGGAAAAACTAACATCTATGT
GAGAGAAGGAAAAATTGTAG

Product: metabolite transport protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKEETKVSIAAMIGIAFEFYDFLIFGFVAGILANLFFPSGNKIVSLLDTFAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IITMTLMGLSSLFTGLLPGYAILGVLAPTLLTILRLLQGFSLGGEFGGGITLSAEFAEPGKRAFYTGIAQMAQGIGPLMA
TGLIFVFSSIMLPSAFASIGWRILFIIGAFIAVIGVMIRLKISESPVFKKVKEKNEISHFPLVDAFRIYWKRILLGLGFI
IGGTTMTYATSVFASSYLENVIGVPARLVSLALTIGYIVEAISILLFAMLADKVGRKPLMVATAVGLLILVYPYFLLIST
GQFSLILLAQIMYSVIGSMSTGAYATALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MILTLIAYLLYKETKGTVFEGQEVLKR

Sequences:

>Translated_427_residues
MKEETKVSIAAMIGIAFEFYDFLIFGFVAGILANLFFPSGNKIVSLLDTFAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IITMTLMGLSSLFTGLLPGYAILGVLAPTLLTILRLLQGFSLGGEFGGGITLSAEFAEPGKRAFYTGIAQMAQGIGPLMA
TGLIFVFSSIMLPSAFASIGWRILFIIGAFIAVIGVMIRLKISESPVFKKVKEKNEISHFPLVDAFRIYWKRILLGLGFI
IGGTTMTYATSVFASSYLENVIGVPARLVSLALTIGYIVEAISILLFAMLADKVGRKPLMVATAVGLLILVYPYFLLIST
GQFSLILLAQIMYSVIGSMSTGAYATALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MILTLIAYLLYKETKGTVFEGQEVLKR
>Mature_427_residues
MKEETKVSIAAMIGIAFEFYDFLIFGFVAGILANLFFPSGNKIVSLLDTFAVFATGFAGRPLGAIVFGHLGDKIGRKYTL
IITMTLMGLSSLFTGLLPGYAILGVLAPTLLTILRLLQGFSLGGEFGGGITLSAEFAEPGKRAFYTGIAQMAQGIGPLMA
TGLIFVFSSIMLPSAFASIGWRILFIIGAFIAVIGVMIRLKISESPVFKKVKEKNEISHFPLVDAFRIYWKRILLGLGFI
IGGTTMTYATSVFASSYLENVIGVPARLVSLALTIGYIVEAISILLFAMLADKVGRKPLMVATAVGLLILVYPYFLLIST
GQFSLILLAQIMYSVIGSMSTGAYATALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAG
MILTLIAYLLYKETKGTVFEGQEVLKR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=32.4009324009324, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=35.3080568720379, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=31.7647058823529, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=29.8368298368298, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=22.7979274611399, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46093; Mature: 46093

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHCC
>Mature Secondary Structure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CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]